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Construction of conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism

Published online by Cambridge University Press:  27 June 2008

Chen Fen
Affiliation:
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Xiong Wei
Affiliation:
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China
Min Yong
Affiliation:
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Fan Yu-Qing
Affiliation:
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Liang Yun-Xiang
Affiliation:
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Lü He-Ping
Affiliation:
Beijing Institute of Biomedicine, Beijing 100091, China
Xing Ren-Chang
Affiliation:
Beijing Institute of Biomedicine, Beijing 100091, China
Zheng Ying-Hua
Affiliation:
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China Beijing Institute of Biomedicine, Beijing 100091, China
Corresponding
E-mail address:

Abstract

Intergeneric transfer of plasmid vectors pSET152 and pHL212 from donor Escherichia coli ET12567/pUZ8002 and S17-1 to Streptomyces cinnamonensis was demonstrated and optimized. Assisted by this conjugation system, nsdA gene disruption was achieved through PCR-targeted gene replacement. One AprRKanS exconjugant BIB309 was then isolated and confirmed to be the nsdA null mutant. Compared with the starting strain, monensin production by the nsdA mutant BIB309 increased 270% in vitro.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(6): 1042–1047

References

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