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Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense

Published online by Cambridge University Press:  12 February 2007

Du Xiong-Ming
Affiliation:
Cotton Research Institute of the Chinese Academy of Agricultural Sciences, Anyang 455000, China
Pan Zhao-E
Affiliation:
Cotton Research Institute of the Chinese Academy of Agricultural Sciences, Anyang 455000, China
Sun Jun-Ling
Affiliation:
Cotton Research Institute of the Chinese Academy of Agricultural Sciences, Anyang 455000, China
Zhou Zhong-Li
Affiliation:
Cotton Research Institute of the Chinese Academy of Agricultural Sciences, Anyang 455000, China
Pang Bao-Yin
Affiliation:
Cotton Research Institute of the Chinese Academy of Agricultural Sciences, Anyang 455000, China
Corresponding
E-mail address:

Abstract

Four variant lines, HB1, HB2, HB3 and HB4, were derived from progenies of upland cotton (Gossypium hirsutum) cultivar Yumian 17 that had been transformed with genomic DNA from sea island cotton, Gossypium barbadense, using the pollen tube pathway (PTP) method. They showed significant differences from the wild-type recipient in terms of fibre quality, lint percentage, boll weight and other agronomic traits. The four variant lines also differed from the wild-type recipient in the amplification products of two SSR loci, indicating that the DNA of G. barbadense had been introduced into the genome of the recipient and was inherited stably. On the other hand, the agronomic traits of another three lines (HB5, HB6 and HB7) derived from the same transformation experiment were identical to those of the wild-type recipient, indicating that G. barbadense DNA had not integrated into the genome of these lines. A combination of the analyses on agronomic traits and SSR markers indicated that transgenic upland cotton strains produced by the PTP method could become homozygous in only a few generations and the foreign DNA introduced during the transformation experiments could be inherited stably in the transgenic progenies.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Czernilofsky, AP, Hain, R, Herrera-Estrella, L, Lorz, H, Goyvaerts, E, Baker, BJ et al. , (1986) Fate of selectable marker DNA integrated into the genome of Nicotiana tabacum. DNA 5: 101113.CrossRefGoogle ScholarPubMed
Duan, XF and Chen, SB (1985) Character variation of transformation exogenous DNA in rice. Scientia Agricultura Sinica 3: 610 (in Chinese with English abstract).Google Scholar
Fu, J, Yan, QQ and Xu, QG (1990) A simple way of introduction of exogenous DNA and induce variation in rice. Hunan Agricultural Science 2: 1820 (in Chinese with English abstract).Google Scholar
Huang, JQ, Qian, SY, Liu, GL, Weng, J, Zheng, YS and Zhou, GY (1981) Variations in the characters of upland cotton (Gossypium hirsutum) induced by exotic DNA of sea-island cotton (Gossypium barbadense). Acta Genetica Sinica 8(1): 5662 (in Chinese with English abstract).Google Scholar
Huang, JQ, Qian, SY, Liu, GL, Weng, J, Zheng, YS and Zhou, GY (1982) Variations in the characters of Gossypium arboreum induced by exotic DNA of sea-island cotton (Gossypium barbadense). Jiangsu Agricultural Science 11: 1821 (in Chinese with English abstract).Google Scholar
Jia, J (1996) Molecular germplasm diagnostics and molecular marker-assisted breeding. Scientia Agricultura Sinica 29(4): 110 (in Chinese with English abstract).Google Scholar
Kohli, A, Griffiths, S, Palacios, N, Twyman, RM, Vain, P, Laurie, DA et al. , (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology-mediated recombination. Plant Journal 17: 591601.CrossRefGoogle ScholarPubMed
Paterson, AH, Brubaker, CL and Wendel, JF (1993) A rapid method for extraction of cotton genomic DNA suitable for RFLP or PCR analysis. Plant Molecular Biology Report 11(2): 122127 (in Chinese with English abstract).CrossRefGoogle Scholar
Singh, RJ, Klein, TM, Manvais, CJ, Knowlton, S, Hymowitz, T and Kostow, CM (1998) Cytological characterization of transgenic soybean. Theoretical and Applied Genetics 96: 319324.CrossRefGoogle Scholar
Wang, SC, Wang, GY, Ding, QX, Zhang, H, Xie, YJ and Dai, JR (1999) Studies of transgenic segregation and integration in maize. Acta Genetica Sinica 26(3): 254261 (in Chinese with English abstract).Google Scholar
Weng, J, Shen, WF, Wang, ZF, Chen, KQ, Yang, WX, Gong, ZZ et al. , (1984) Molecular proof of exogenous DNA transformation to cotton. Acta Biochemica and Biophysica 16(3): 325327 (in Chinese with English abstract).Google Scholar
Wu, X (1984) Research on variated effects of Gossypium hirsutum induced by multiple exogenous DNA. Hunan Agricultural Science 73(4): 57 (in Chinese with English abstract).Google Scholar
Xia, LQ and Guo, SD (2001) Integration and genetic stability of BT gene in the transgenic cotton with double pest-resistant genes. Chinese Science Bulletin 46(7): 565568 (in Chinese with English abstract).Google Scholar
Xinjian Production and Construction Corps (1999) Cotton germplasm enhancement by transformation of exogenous DNA. Crop Genetic Resources 69(3): 45 (in Chinese with English abstract).Google Scholar
Zhang, J, Wu, YT, Guo, WZ and Zhang, TZ (2000) Fast screening of microsatellite markers in cotton with PAGE/silver staining. Cotton Science 12(5): 267269 (in Chinese with English abstract).Google Scholar
Zheng, HJ, Yu, YJ, Yin, CY, Zhang, LX and Li, B (2002) Identification of dwarf variants of wheat by RAPD method and their genetics analysis. Acta Botanica Boreali-Occidentalia Sinica 22(4): 758765 (in Chinese with English abstract).Google Scholar
Zhou, GY, Weng, J, Huang, JQ, Zheng, YS, Qian, SY and Liu, GL (1983a) Exogenous DNA mediated phenotypic variation in cotton. In: Goldberg, RB (editor) Plant Molecular Biology. New York: Alan R Liss.Google Scholar
Zhou, G, Weng, J, Zeng, Y, Huang, J, Qian, S and Liu, G (1983b) Introduction of exogenous DNA into cotton embryos. Methods in Enzymology 101: 433481.CrossRefGoogle ScholarPubMed

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Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense
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Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense
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