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Staphylococcus aureus adheres avidly to decellularised cardiac homograft tissue in vitro in the fibrinogen-dependent manner

Published online by Cambridge University Press:  21 September 2020

Bartosz Ditkowski Open the ORCID record for Bartosz Ditkowski [Opens in a new window] ,
Kirsten Leeten Open the ORCID record for Kirsten Leeten [Opens in a new window] ,
Ramadan Jashari ,
Elizabeth Jones  and
Ruth Heying Open the ORCID record for Ruth Heying [Opens in a new window]
Bartosz Ditkowski*
Affiliation:
Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium
Kirsten Leeten
Affiliation:
Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium
Ramadan Jashari
Affiliation:
European Homograft Bank, Saint Jean Clinique, Brussels, Belgium
Elizabeth Jones
Affiliation:
Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium
Ruth Heying
Affiliation:
Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium
*
Author for correspondence: Bartosz Ditkowski, PhD, Cardiovascular Developmental Biology/Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Herestraat 49-3000, Leuven, Belgium. E-mail: bartosz.ditkowski@gmail.com
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Abstract

Objective:

Infective endocarditis remains a severe complication associated with a high morbidity and mortality in patients after heart valve replacement. Exploration of the pathogenesis is of high demand and we, therefore, present a competent model that allows studying bacterial adherence and the role of plasma fibrinogen in this process using a new in-house designed low-volume flow chamber. Three cardiac graft tissues used for pulmonary valve replacement have been tested under shear conditions to investigate the impact of surface composition on the adhesion events.

Methods:

Tissue pieces of cryopreserved homograft (non-decellularised), decellularised homograft and bovine pericardium patch were investigated for fibrinogen binding. Adherence of Staphylococcus aureus to these graft tissues was studied quantitatively under flow conditions in our newly fabricated chamber based on a parallel plates’ modality. The method of counting colony-forming units was reliable and reproducible to assess the propensity of different graft materials for bacterial attachment under shear.

Results:

Bacterial perfusions over all plasma-precoated tissues identified cryopreserved homograft with the lowest affinity for S. aureus compared to decellularised homograft presenting a significantly higher bacterial adhesion (p < 0.05), which was linked to a more avid fibrinogen binding (p < 0.01). Bovine pericardial patch, as a reference tissue in this study, was confirmed to be the most susceptible tissue graft for the bacterial adhesion, which was in line with our previous work.

Conclusion:

The two studied homograft tissues showed different levels of bacterial attachment, which might be postulated by the involvement of fibrinogen in the adhesion mechanism(s) shown previously for bovine tissues.

Keywords

Bacterial adhesioncardiac graft tissuesendothelial cellsflow chamberinfective endocarditisS. aureussubendothelial matrixshear stress
Type
Original Article
Information
Cardiology in the Young , Volume 30 , Issue 12 , December 2020 , pp. 1783 - 1787
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Copyright
© The Author(s), 2020. Published by Cambridge University Press

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