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Mild Hypothermia Preserves Contractile Function and Inhibits Prostaglandin E2 Release from Metabolically Stressed Skeletal Muscle

Published online by Cambridge University Press:  18 September 2015

Valerie A. Cwik ,
Ramanath Majumdar  and
Michael H. Brooke
Valerie A. Cwik*
Affiliation:
Division of Neurology, the University of Alberta, Edmonton, Alberta
Ramanath Majumdar
Affiliation:
Division of Neurology, the University of Alberta, Edmonton, Alberta
Michael H. Brooke
Affiliation:
Division of Neurology, the University of Alberta, Edmonton, Alberta
*
Division of Neurology, 2E3.13 Walter MacKenzie Center, University of Alberta, 8440 - 112th Street, Edmonton, Alberta, Canada T6G 2B7
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Abstract:

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An in vitro model of muscle damage was used to investigate the protective effect of mild hypothermia in muscle injury. Rat epitrochlearis muscles were dissected in their entirety and suspended in Krebs-Ringer solution and DNP, a mitochondrial uncoupler, was added. PGE2, and lactate release and the contractile response to stimulation were measured and compared to untreated controls. Experiments were done at 37, 35, 33 and 27°C. At 37°C, DNP stimulated muscle releases large amounts of PGE2 and lactate and is unable to contract. As the temperature is reduced, there is progressive preservation of contractile force, although high lactate levels at the lowest temperatures indicate that the metabolic stress is still present. In contrast, DNP stimulated PGE2 release is completely inhibited at or below 35°C and may be related to a similar protective phenomenon seen in experimental ischemic neuronal death.

Résumé:

RÉSUMÉ:

Nous avons utilisé un modèle in vitro de lésion musculaire pour investiguer l'effet protecteur d'une légère hypothermie dans le traumatisme musculaire. Des muscles épitrochléens de rat ont été disséqués et suspendus dans une solution de Krebs-Ringer à laquelle du DNP, un découpleur mitochondrial, a été ajouté. La libération de PGE2 et de lactate ainsi que la réponse contractile à la stimulation ont été mesurées et comparées à celles de contrôles non traités. Les essais étaient faits à 37, 35, 33 et 27°C. A 37°C, le muscle stimulé par le DNP libère de grandes quantités de PGE2 et de lactate et il est incapable de se contracter. A mesure que la température est abaissée, on observe une préservation progressive de la force contractile, bien que de fortes concentrations de lactate aux températures les plus basses indiquent que le stress métabolique est encore présent. Par contre, la stimulation de la libération de PGE2 par le DNP est complètement inhibée à des températures de 35°C ou moins et peut être reliée à un phénomène protecteur semblable à celui observé dans la mort neuronale expérimentale par ischémie.

Type
Articles
Information
Canadian Journal of Neurological Sciences , Volume 21 , Issue 2 , May 1994 , pp. 120 - 124
Copyright
Copyright © Canadian Neurological Sciences Federation 1994

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