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Acidic Fibroblast Growth Factor Infusion Reduces Ischemic CA1 Hippocampal Damage in the Gerbil

Published online by Cambridge University Press:  18 September 2015

V. MacMillan ,
K. Walton-Roche  and
J. Davis
V. MacMillan*
Affiliation:
Division of Neurology, University of Toronto, Toronto (V.M.) and the Department of Medicine (Neurology). Duke University, Durham, North Carolina (K.W-R, J.D.)
K. Walton-Roche
Affiliation:
Division of Neurology, University of Toronto, Toronto (V.M.) and the Department of Medicine (Neurology). Duke University, Durham, North Carolina (K.W-R, J.D.)
J. Davis
Affiliation:
Division of Neurology, University of Toronto, Toronto (V.M.) and the Department of Medicine (Neurology). Duke University, Durham, North Carolina (K.W-R, J.D.)
*
Room 6366, Medical Science Building, University of Toronto, Toronto, Ontario, Canada M5S 1A8
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Abstract:

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Occlusion of the carotid arteries for 5 minutes in the Mongolian gerbil results in selective necrosis of CAI pyramidal neurons. In the present experiments we studied whether intraventricular infusion of acidic fibroblast growth factor (aFGF) could attentuate this damage. Intraventricular infusions of bovine serum albumin (BSA-10 ng/h) or aFGF (1, 10 or 100 ng/h) were started 2 days prior to 5 minutes of bilateral carotid occlusion and continued for 5 days post-ischemia. The brains were perfused and fixed at 5 days post-ischemia and histological assessment of CAI damage was made. Animals receiving intraventricular infusions of 10 or 100 ng/h aFGF showed a significant reduction of CA1 neuronal damage in comparison to no treatment ischemic controls (no treatment − 8 + 1 ; aFGF 10 ng/h − 147 ± 28; aFGF 100 ng/h − 168 ± 35 cells/mm CAI; P < 0.05 for both aFGF groups). The results indicate that aFGF infusion can attenuate the severity of ischemic neuronal necrosis in the gerbil hippocampus.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 20 , Issue 1 , February 1993 , pp. 37 - 40
Copyright
Copyright © Canadian Neurological Sciences Federation 1993

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