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Host-range testing of a mixture of two nucleopolyhedroviruses of Choristoneura fumiferana (Lepidoptera: Tortricidae)

Published online by Cambridge University Press:  03 January 2012

William J. Kaupp ,
Kevin N. Barber ,
William E. Fick ,
Peter M. Ebling ,
Tim R. Ladd  and
Stephen B. Holmes
William J. Kaupp
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Kevin N. Barber
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
William E. Fick
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Peter M. Ebling
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Tim R. Ladd
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Stephen B. Holmes*
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
*
2Corresponding author (e-mail: steve.holmes@nrcan.gc.ca).
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Abstract

The host range of a mixture of Choristoneura fumiferana (Clemens) nucleopolyhedroviruses (CfMNPV and CfDefNPV) was investigated using a per os bioassay of larvae of 29 species of Lepidoptera and adult males of Megachile rotundata (F.) (Hymenoptera: Megachilidae). Using a whole-genomic DNA probe, positive results were obtained in 8 of 10 Tortricidae: Archips cerasivorana (Fitch), Choristoneura fractivittana (Clemens), C. fumiferana, Choristoneura occidentalis Freeman, Choristoneura pinus pinus Freeman, Choristoneura rosaceana (Harris), Clepsis persicana (Fitch), and Cydia pomonella (L.); one Crambidae: Ostrinia nubilalis (Hübner); one arctiine Erebidae: Estigmene acrea (Drury); and two Noctuidae: Oligia illocata (Walker) and Pyrrhia exprimens (Walker). Mortality rates were highest among C. fumiferana, C. occidentalis, C. pinus pinus, A. cerasivorana, and C. pomonella. Sequenced polymerase chain reaction (PCR) amplicons from infected individuals from several species confirmed that the primer sets amplified the target viruses. CfMNPV was consistently found in virus-fed C. fumiferana; whereas, CfDefNPV was present only occasionally. The presence of CfMNPV and CfDefNPV in A. cerasivorana was confirmed by PCR and DNA sequencing. Significant treatment-mortality rates were induced in the noctuids P. exprimens and Acronicta impleta Walker; PCR determined that both viruses were present in treated P. exprimens but only CfMNPV was present in A. impleta. No virus was detected in M. rotundata.

Résumé

Nous avons étudié la gamme des hôtes d’un mélange de virus de la nucléopolyhédrose (CfMNPV et CfDefNPV) de Choristoneura fumiferana (Clemens) au moyen de bioessais par voie orale chez des larves de 29 espèces de lépidoptères et des mâles adultes de Megachile rotundata (F.) (Hymenoptera : Megachilidae). À l’aide d’une sonde ADN de génome entier, nous avons obtenu des résultats positifs chez 8 de 10 tortricidés, Archips cerasivorana (Fitch), Choristoneura fractivittana (Clemens), C. fumiferana, Choristoneura occidentalis Freeman, Choristoneura pinus pinus Freeman, Choristoneura rosaceana (Harris), Clepsis persicana (Fitch) et Cydia pomonella (L.) ; un crambidé, Ostrinia nubilalis (Hübner) ; un érebidé arctiiné, Estigmene acrea (Drury) ; et deux noctuidés, Oligia illocata (Walker) et Pyrrhia exprimens (Walker). Les taux de mortalité sont maximaux chez C. fumiferana, C. occidentalis, C. pinus pinus, A. cerasivorana et C. pomonella. Les amplicons séquencés de l’amplification en chaîne par polymérase (PCR) provenant d’individus infectés appartenant à plusieurs espèces confirment que les jeux d’amorces amplifient les virus ciblés. Le CfMNPV se retrouve constamment chez les C. fumifera nourris de virus, alors que le CfDefNPV n’apparaît qu’occasionnellement. La présence du CfMNPV et du CfDefNPV chez A. cerasivorana est confirmée par la PCR et le séquençage d’ADN. Des taux de mortalité significatifs reliés au traitement ont été provoqués chez les noctuidés P. exprimens et Acronicta impleta Walker; la PCR a déterminé que les deux virus sont présents chez les P. exprimens traités, mais seul CfMNPV apparaît chez A. impleta. Aucun virus n’a été décelé chez M. rotundata.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 143 , Issue 2 , April 2011 , pp. 165 - 177
Copyright
Copyright © Entomological Society of Canada 2011

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