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LACK OF CLONAL VARIATION AMONG CANADIAN POPULATIONS OF THE CORN LEAF APHID, RHOPALOSIPHUM MAIDIS FITCH (HOMOPTERA: APHIDIDAE)

Published online by Cambridge University Press:  31 May 2012

J.C. Simon*
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
P.D.N. Hebert
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
C. Carillo
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
R. De Melo
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
*
1 Author to whom correspondence should be addressed. Present address: Lahoratoire de Zoologie, Institut National de la Recherche Agronomique, B.P. 29 35650 Le Rheu, FRANCE.

Abstract

The extent of allozyme variation and the amount of genetic divergence were assessed among populations of the aphid Rhopalosiphum maidis in Ontario. Allozyme analysis of individuals from 12 sites revealed no polymorphism for 32 loci. In addition, the extent of karyotypic, allozyme, and mitochondrial DNA (mtDNA) variation was examined for clones from North America, Europe, and North Africa. As previously noted, chromosome number varied in relation to the host-plant on which aphids were collected, but neither allozyme nor mtDNA patterns allowed discrimination among clones. Further work is needed to determine whether the lack of genetic diversity in R. maidis is linked with its recent introduction to North America or with its loss of a sexual phase.

Résumé

Les niveaux de polymorphisme enzymatique et de différenciation génétique ont été mesurés pour des populations ontariennes du puceron Rhopalosiphum maidis. L’analyse d’individus collectés dans 12 sites différents n’a révélé aucun polymorphisme aux 32 locus étudiés. En complément, les variations au niveau du nombre de chromosomes, des allozymes et de l’ADN mitochondrial (ADNmt) ont été analysées sur un échantillon de clones d’Amérique du Nord, d’Europe et d’Afrique du Nord. Nous avons confirmé que le nombre de chromosomes des clones de R. maidis est associé à la plante-hôte sur laquelle ils ont été récoltés, mais ni l’analyse des allozymes ni celle de l’ADNmt n’ont permis de différencier les clones. Des travaux complémentaires sont nécessaires afin de déterminer si l’absence de diversité génétique chez R. maidis est liée à sa récente introduction en Amérique du Nord ou à la perte de la sexualité chez cette espèce.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1995

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LACK OF CLONAL VARIATION AMONG CANADIAN POPULATIONS OF THE CORN LEAF APHID, RHOPALOSIPHUM MAIDIS FITCH (HOMOPTERA: APHIDIDAE)
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