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Variation between biotype E clones of Schizaphis graminum (Homoptera: Aphididae)

Published online by Cambridge University Press:  10 July 2009

Kevin A. Shufran ,
David C. Margolies  and
William C. Black IV
Kevin A. Shufran*
Affiliation:
Department of Entomology, Kansas State University, Manhattan, Kansas, USA
David C. Margolies
Affiliation:
Department of Entomology, Kansas State University, Manhattan, Kansas, USA
William C. Black IV
Affiliation:
Department of Entomology, Kansas State University, Manhattan, Kansas, USA
*
Kevin A. Shufran, Pesticide Research Center, Michigan State University, East Lansing, Michigan 48824–1115, USA.
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Abstract

Phenotypic and genotypic variation was investigated between biotype E clones of the greenbug, Schizaphis graminum (Rondani). S. graminum was collected from wheat and sorghum fields in seven Kansas counties, USA. Colonies representing single maternal lineages (clones) were initiated and maintained in an insectary, and were verified as biotype E. An allozyme survey among clones did not detect any polymorphism at 24 enzymatic loci. We were unable to detect interclonal variation for nymphal survivorship, age at first reproduction, fecundity, and reproductive lifespan. Variation among genetically homogeneous daughters within clones was as great as variation among different clones for the above parameters, due to microenvironmental effects. However, by using the intergenic spacer in the rDNA cistron as a molecular fingerprinting tool, we identified 56 unique maternal lineages among 61 clones tested. The length of the intergenic spacer varied within individual S. graminum, and different maternal lineages had unique sets of spacer sizes. However, S. graminum within a single clone shared the same sets of spacer sizes. Extensive molecular genetic variation was found among biotype E clones, despite a lack of variation in additive genetic traits. Thus, biotype E populations are made up of many genetically distinct clones and do not represent an asexual proliferation of one or a few maternal lineages.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 82 , Issue 3 , September 1992 , pp. 407 - 416
Copyright
Copyright © Cambridge University Press 1992

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