Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-07-07T04:13:53.314Z Has data issue: false hasContentIssue false
  • English
  • Français

Quick detection of Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) in chestnut dormant buds by nested PCR

Published online by Cambridge University Press:  27 January 2012

C. Sartor ,
D. Torello Marinoni ,
A. Quacchia  and
R. Botta
C. Sartor*
Affiliation:
Dipartimento di Colture Arboree,Università di Torino, via Leonardo da Vinci 44, Grugliasco 10095, Torino, Italy
D. Torello Marinoni
Affiliation:
Dipartimento di Colture Arboree,Università di Torino, via Leonardo da Vinci 44, Grugliasco 10095, Torino, Italy
A. Quacchia
Affiliation:
DIVAPRA, Settore di Entomologia e Zoologia Applicate all'Ambiente ‘C. Vidano’, Università di Torino, Via Leonardo da Vinci 44, Grugliasco 10095 Torino, Italy
R. Botta
Affiliation:
Dipartimento di Colture Arboree,Università di Torino, via Leonardo da Vinci 44, Grugliasco 10095, Torino, Italy
*
*Author for correspondence Fax: +390116708658 E-mail: chiara.sartor@unito.it
Get access Rights & Permissions [Opens in a new window]

Abstract

Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) develops in chestnut buds that remain asymptomatic from oviposition (June–July) until budburst; it is, thus, easily spread by plant material used in propagation. Therefore, it is particularly interesting to identify infested plant batches before their movement. Unfortunately, a non-destructive method for checking buds has not yet been developed, and the only technique available is the screening of a bud sample. The visual investigation is long and requires highly skilled and trained staff. The purpose of this work was to set up an effective and fast method able to identify the presence of first instar larvae of D. kuriphilus in a large number of chestnut buds by PCR. Four primer pairs were designed on nuclear and mitochondrial sequences of a set of seven gall wasp taxa and tested on five different cynipid's DNA. Nested diagnostic PCR was carried out on DNA extracted from samples of 2 g buds simulating four levels of infestation (larvae were added to uninfested buds); 320 bp amplicon of 28S sequence was chosen as a marker to detect one larva out of 2 g buds. The method showed a potential efficiency of 5000 to 15,000 buds per week, depending on bud size.

Keywords

gall waspCastaneaDNA28Sdiagnosis
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 3 , June 2012 , pp. 367 - 371
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aebi, A., Schönrogge, K., Melika, G., Alma, A., Bosio, G., Quacchia, A., Picciau, L., Abe, Y., Moriya, S., Yara, K., Seljak, G. & Stone, G.N. (2006) Parasioid Recruitment to the globally invasive chestnut gall wasp Dryocosmus kuriphilus. pp. 103–121in Ozaki, K., Yukawa, J., Ohgushi, T. & Price, P.W. (Eds) Ecology and Evolution of Galling Arthropods and their Associates. Tokyo, Japan, Springer-Verlag.Google Scholar
Ballard, J.W.O., Olsen, G.J., Faith, D.P., Odgers, W.A., Rowell, D.M. & Atkinson, P.W. (1992) Evidence from 12S ribosomal RNA sequences that onychophorans are modified arthropods. Science 258, 1345–1348.CrossRefGoogle ScholarPubMed
Hwang, U.-W. & Kim, W. (1999) General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics. The Korean Journal of Parasitology 37(4), 215–228.Google Scholar
Payne, J.A., Menke, A.S. & Schroeder, P.M. (1975) Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), an Oriental chestnut gall wasp in North America. USDA Cooperative Economic Insect Report 25, 903–905.Google Scholar
Quacchia, A., Moriya, S., Bosio, G., Scapin, I. & Alma, A. (2008) Rearing, release and settlement prospect in Italy of Torymus sinensis, the biological control agent of the chestnut gall wasp Dryocosmus kuriphilus. BioControl 53, 829–839.Google Scholar
Rokas, A., Nylander, J.A.A., Ronquist, F. & Stone, G.N. (2002) A maximum Likelihood analysis of eight Phylogenetic markers in gallwasps (Hymenoptera: Cynipidae) implication for insect Phylogentic studies. Molecular Phylogenetics and Evolution 22(2), 206–219.CrossRefGoogle Scholar
Simon, C., Franke, A. & Martin, A. (1991) The polymerase chain reaction: DNA extraction and amplification. pp. 329–355in Hewitt, G.H., Johnston, A.W.B. & Young, J.P.W. (Eds) Molecular Techniques in Taxonomy. Berlin, Germany, Springer-Verlag.Google Scholar
Stone, G.N. & Cook, J.M. (1998) The structure of cynipid oak galls: patterns in the evolution of an extended phenotype. Proceedings of the Royal Society of London 265, 979–988.CrossRefGoogle Scholar
Stone, G.N., Schönrogge, K., Atkinson, R.J., Bellido, D. & Pujade-Villar, J. (2002) The population biology of oak gall wasps (Hymenoptera: Cynipidae). Annual Review of Entomology 47, 633–668.CrossRefGoogle ScholarPubMed
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Resources 22, 4673–4680.Google Scholar
Wolstenholme, D.R. (1992) Animal mitochondrial DNA: structure and evolution. International Review of Cytology 141, 173–216.Google Scholar