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Heteroplasmy due to coexistence of mtCOI haplotypes from different lineages of the Thrips tabaci cryptic species group

Published online by Cambridge University Press:  31 January 2017

S.J. Gawande ,
S. Anandhan ,
A.A. Ingle ,
Alana Jacobson  and
R. Asokan
S.J. Gawande*
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune 410505, India
S. Anandhan
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune 410505, India
A.A. Ingle
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune 410505, India
Alana Jacobson
Affiliation:
Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama 36849, 334-844-5011, USA
R. Asokan
Affiliation:
Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessarghatta Lake, Bangalore 560089, India
*
*Author for correspondence Fax: +91-2135-224056 Phone: +91-2135-222026 E-mail: sureshgawande76@gmail.com
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Abstract

Heteroplasmy is the existence of multiple mitochondrial DNA haplotypes within the cell. Although the number of reports of heteroplasmy is increasing for arthropods, the occurrence, number of variants, and origins are not well studied. In this research, the occurrence of heteroplasmy was investigated in Thrips tabaci, a putative species complex whose lineages can be distinguished by their mitochondrial DNA haplotypes. The results from this study showed that heteroplasmy was due to the occurrence of mitochondrial cytochrome oxydase I (mtCOI) haplotypes from two different T. tabaci lineages. An assay using flow cytometry and quantitative real-time PCR was then used to quantify the per cell copy number of the two mtCOI haplotypes present in individuals exhibiting heteroplasmy from nine geographically distant populations in India. All of the T. tabaci individuals in this study were found to exhibit heteroplasmy, and in every individual the per cell copy number of mtCOI from lineage 3 comprised 75–98% of the haplotypes detected and was variable among individuals tested. There was no evidence to suggest that the presense of lineage-specific haplotypes was due to nuclear introgression; however, further studies are needed to investigate nuclear introgression and paternal leakage during rare interbreeding between individuals from lineages 2 and 3.

Keywords

heteroplasmyThrips tabacisex lineagesarrhenotokythelytoky
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 4 , August 2017 , pp. 534 - 542
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Copyright
Copyright © Cambridge University Press 2017 

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