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DNA-based identifications reveal multiple introductions of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae) into the Torres Strait Islands and Papua New Guinea

Published online by Cambridge University Press:  20 May 2015

M.J. Blacket ,
A.D. Rice ,
L. Semeraro  and
M.B. Malipatil
M.J. Blacket*
Affiliation:
Department of Economic Development (DEDJTR), Biosciences Research Division (BRD), AgriBio, Centre for AgriBioscience, Bundoora, Victoria 3083, Australia
A.D. Rice
Affiliation:
Department of Agriculture, Northern Australian Quarantine Strategy (NAQS), Cairns, Queensland 4870, Australia
L. Semeraro
Affiliation:
Department of Economic Development (DEDJTR), Biosciences Research Division (BRD), AgriBio, Centre for AgriBioscience, Bundoora, Victoria 3083, Australia
M.B. Malipatil
Affiliation:
Department of Economic Development (DEDJTR), Biosciences Research Division (BRD), AgriBio, Centre for AgriBioscience, Bundoora, Victoria 3083, Australia La Trobe University, Bundoora, Victoria 3086, Australia
*
*Author for correspondence Phone: +61 (03)-9032-7333 Fax: +61 (03)-9032-7604 E-mail: mark.blacket@ecodev.vic.gov.au
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Abstract

Leafmining flies (Diptera: Agromyzidae) can be serious economic pests of horticultural crops. Some genera such as Liriomyza are particularly problematic with numerous species, some of which are highly polyphagous (wide host range), which can only be confidently identified morphologically from adult males. In our study, DNA barcoding was employed to establish new locality records of the vegetable leafminer fly, Liriomyza sativae, from the islands of Torres Strait (Queensland, Australia) and the central highlands of Papua New Guinea (PNG). These records represent significant range extensions of this highly invasive plant pest. Specimens of immature leafminers (from leaf mines) were collected over a 5-year period during routine plant health surveys in ethanol or on FTA® filter paper cards, both methods proved effective at preserving and transporting insect DNA under tropical conditions, with FTA cards possessing some additional logistical benefits. Specimens were identified through sequencing two sections of the cytochrome oxidase I gene and the utility of each was assessed for the identification of species and intra-specific genetic lineages. Our study indicates that multiple haplotypes of L. sativae occur in PNG, while a different haplotype is present in the Torres Strait, with genetic regionalization between these areas apart from a single possible instance – one haplotype ‘S.7’ appears to be common between these two regions – interestingly this has also been the most common haplotype detected in previous studies of invasive L. sativae populations. The DNA barcoding methods employed here not only identified multiple introductions of L. sativae, but also appear generally applicable to the identification of other agromyzid leafminers (Phytomyzinae and Agromyzinae) and should decrease the likelihood of potentially co-amplifying internal hymenopteran parasitoids. Currently, L. sativae is still not recorded from the Australian mainland; however, further sampling of leafminer flies from Northern Australia and surrounding areas is required, as surveillance for possible Liriomyza incursions, as well as to characterize endemic species with which Liriomyza species might be confused.

Keywords

Leafmining flyLiriomyza sativaeNew records: Torres Strait IslandsPapua New Guinea (PNG)Quarantine/Biosecurity: invasive plant pestDNA barcoding: COI geneDNA preservation: FTA cards
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 5 , October 2015 , pp. 533 - 544
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Copyright
Copyright © Cambridge University Press 2015 

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