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Characterization and polymorphism analysis of phosphoglucose isomerase gene in the fall webworm (Hyphantria cunea)

Published online by Cambridge University Press:  07 February 2012

J. Luo ,
X.-Y. Cheng ,
X. Yan ,
W.-Q. Tao ,
J.D. Holland  and
R.-M. Xu
J. Luo
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
X.-Y. Cheng*
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
X. Yan
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
W.-Q. Tao
Affiliation:
Beijing Municipal Bureau of Landscape and Forestry, 8 Yumin Middle Road, Beijing, 100029, China
J.D. Holland
Affiliation:
Department of Entomology, Purdue University, West Lafayette, IN 47907, USA
R.-M. Xu
Affiliation:
College of Life Sciences, Beijing Normal University, 19 Xinjiekou Wai Dajie, Beijing, 100875, China
*
*Author for correspondence Fax: +86-10-58807721 E-mail: chengxy@bnu.edu.cn
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Abstract

Phosphoglucose isomerase (PGI) plays an important role in energy metabolism, and it is documented that PGI exhibits an extensive polymorphism which can affect insects' fitness and adaptation. In this paper, we studied the structural characteristics and polymorphism of pgi gene in the fall webworm (Hyphantria cunea), an important invasive pest in some European and Asian countries. A 2110-bp pgi full-length cDNA encoding a polypeptide of 556 amino acids was obtained from H. cunea. The pgi full-length in the H. cunea genomic DNA was 14,332 bp with 12 exons and 11 introns, similar to the structures of pgi in other Lepidoptera species. We compared the structures of pgi in different insect species. Moreover, thirteen pgi genotypes comprised of five alleles were identified in the Chinese population. Genotypes pgi-cd, pgi-cc and pgi-ce were the most prevalent with over 70% of individuals allocated to them. Four out of five alleles were sequenced the cDNA full-length. Thirty stably variable sites were found among them with five non-synonymous mutation sites. The frequencies of alleles and genotypes were variable in different Chinese geographic subpopulations. Moreover, comparison of pgi mRNA expression levels in each stage of the moth's lifecycle showed that a high expression level was in the 6th instar larval stage, followed by that in the egg and adult stages. The results will provide a basis for further study of the role of different alleles and genotypes of PGI on fitness and adaptation of the moth H. cunea.

Keywords

phosphoglucose isomerasepgiallelegenotypepolymorphismHyphantria cunea
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 4 , August 2012 , pp. 477 - 488
Copyright
Copyright © Cambridge University Press 2012

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References

Bambara, S. & Baker, J.R. (2006) Fall Webworm. Ornamentals and Turf. Availalble online at http://www.ces.ncsu.edu/depts/ent/notes/O&T/trees/note46/note46.html (accessed December 2011).Google Scholar
Biswas, S. & Akey, J.M. (2006) Genomic insights into positive selection. Trends in Genetics 22(8), 437–446.CrossRefGoogle ScholarPubMed
Carvalho, G. (1988) Differences in the frequency and fecundity of PGI-marked genotypes in a natural population of Daphnia magna Straus (Crustacea: Cladocera). Functional Ecology 2(4), 453–462.CrossRefGoogle Scholar
Dahlhoff, E.P. & Rank, N.E. (2000) Functional and physiological consequences of genetic variation at phosphoglucose isomerase: Heat shock protein expression is related to enzyme genotype in a montane beetle. Proceedings of the National Academy of Sciences 97(18), 10056–10061.CrossRefGoogle Scholar
Dahlhoff, E.P. & Rank, N.E. (2007) The role of stress proteins in responses of a montane willow leaf beetle to environmental temperature variation. Journal of Biosciences 32(3), 477–488.CrossRefGoogle ScholarPubMed
Dahlhoff, E.P., Fearnley, S.L., Bruce, D.A., Gibbs, A.G., Stoneking, R., McMillan, D.M., Deiner, K., Smiley, J.T. & Rank, N.E. (2008) Effects of Temperature on Physiology and Reproductive Success of a Montane Leaf Beetle: Implications for Persistence of Native Populations Enduring Climate Change. Physiological and Biochemical Zoology 81(6), 718–732.CrossRefGoogle ScholarPubMed
Gomi, T. (2007) Seasonal adaptations of the fall webworm Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) following its invasion of Japan. Ecological Research 22(6), 855–861.CrossRefGoogle Scholar
Gomi, T., Muraji, M. & Takeda, M. (2004) Mitochondrial DNA analysis of the introduced fall webworm, showing its shift in life cycle in Japan. Entomological Science 7(2), 183–188.CrossRefGoogle Scholar
Gomi, T., Adachi, K., Shimizu, A., Tanimoto, K., Kawabata, E. & Takeda, M. (2009) Northerly shift in voltinism watershed in Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) along the Japan Sea coast: evidence of global warming? Applied Entomology and Zoology 44(3), 357–362.CrossRefGoogle Scholar
Haag, C.R., Saastamoinen, M., Marden, J.H. & Hanski, I. (2005) A candidate locus for variation in dispersal rate in a butterfly metapopulation. Proceedings of the Royal Society, Series B: Biological Sciences 272(1580), 2449–2456.Google Scholar
Hanski, I. & Saccheri, I. (2006) Molecular-level variation affects population growth in a butterfly metapopulation. Plos Biology 4, 719–726.CrossRefGoogle Scholar
Ji, R., Xie, B.-Y., Li, X.-H., Gao, Z.-X. & Li, D.-M. (2003) Research progress on the invasive species, Hyphantria cunea. Entomological Knowledge 40(1), 13–18.Google Scholar
Johannesson, K., Kautsky, N. & Tedengren, M. (1990) Genotypic and phenotypic differences between Baltic and North Sea populations of Mytilus edulis evaluated through reciprocal transplantations. II. Genetic variation. Marine Ecolog, Progress Series 59(3), 211–219.CrossRefGoogle Scholar
Karl, I., Schmitt, T. & Fischer, K. (2008) Phosphoglucose isomerase genotype affects life-history traits and cold stress resistance in a Copper butterfly. Functional Ecology 22(5), 887–894.CrossRefGoogle Scholar
Karl, I., Hoffmann, K.H. & Fischer, K. (2010) Food stress sensitivity and flight performance across phosphoglucose isomerase enzyme genotypes in the sooty copper butterfly. Population Ecology 52(2), 307–315.CrossRefGoogle Scholar
Katz, L.A. & Harrison, R.G. (1997) Balancing selection on electrophoretic variation of phosphoglucose isomerase in two species of field cricket: Gryllus veletis and G. pennsylvanicus. Genetics 147(2), 609–621.CrossRefGoogle ScholarPubMed
Kharazipour, A.R., Schöpper, C., Müller, C. & Euring, M. (2009) Review of Forests, Wood Products and Wood Biotechnology of Iran and Germany. Part III. Göttingen, Germany, Universitätsverlag Göttingen.CrossRefGoogle Scholar
Klemme, I. & Hanski, I. (2009) Heritability of and strong single gene (pgi) effects on life-history traits in the Glanville fritillary butterfly. Journal of Evolutionary Biology 22(9), 1944–1953.CrossRefGoogle ScholarPubMed
Kozak, M. (1997) Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6. The EMBO Journal 16(9), 2482–2492.CrossRefGoogle ScholarPubMed
Li, S., Gao, B., Zhang, D., Ning, C. & Qu, J. (2009) Studies of Risk Assessment of Hypanthia cunea (Drury). Chinese Agricultural Science Bulletin 25(10), 202–206.Google Scholar
Li, Y.P., Goto, M., Ito, S., Sato, Y., Sasaki, K. & Goto, N. (2001) Physiology of diapause and cold hardiness in the overwintering pupae of the fall webworm Hyphantria cunea (Lepidoptera: Arctiidae) in Japan. Journal of Insect Physiology 47(10), 1181–1187.CrossRefGoogle ScholarPubMed
Mitikka, V. & Hanski, I. (2010) Pgi genotype influences flight metabolism at the expanding range margin of the European map butterfly. Annales Zoologici Fennici 47(1), 1–14.CrossRefGoogle Scholar
Niitepold, K., Smith, A.D., Osborne, J.L., Reynolds, D.R., Carreck, N.L., Martin, A.P., Marden, J.H., Ovaskainen, O. & Hanski, I. (2009) Flight metabolic rate and pgi genotype influence butterfly dispersal rate in the field. Ecology 90(8), 2223–2232.CrossRefGoogle ScholarPubMed
Orsini, L., Corander, J., Alasentie, A. & Hanski, I. (2008) Genetic spatial structure in a butterfly metapopulation correlates better with past than present demographic structure. Molecular Ecology 17(11), 2629–2642.CrossRefGoogle Scholar
Patarnello, T., Bisol, P.M. & Battaglia, B. (1989) Studies on differential fitness of PGI genotypes with regard to temperature in Gammarus insensibilis (Crustacea: Amphipoda). Marine Biology 102(3), 355–359.CrossRefGoogle Scholar
Pough, F.H. (1989) Organismal performance and Darwinian fitness: approaches and interpretations. Physiological Zoology 62(2), 199–236.CrossRefGoogle Scholar
Rank, N.E., Bruce, D.A., McMillan, D.M., Barclay, C. & Dahlhoff, E.P. (2007) Phosphoglucose isomerase genotype affects running speed and heat shock protein expression after exposure to extreme temperatures in a montane willow beetle. Journal of Experimental Biology 210(5), 750–764.CrossRefGoogle Scholar
Saastamoinen, M. (2007) Life-history, genotypic, and environmental correlates of clutch size in the Glanville fritillary butterfly. Ecological Entomology 32, 235–242.CrossRefGoogle Scholar
Saastamoinen, M. & Hanski, I. (2008) Genotypic and environmental effects on flight activity and oviposition in the Glanville fritillary butterfly. American Naturalist 171(6), 701–712.CrossRefGoogle ScholarPubMed
Saastamoinen, M., Ikonen, S. & Hanski, I. (2009) Significant effects of Pgi genotype and body reserves on lifespan in the Glanville fritillary butterfly. Proceedings of the Royal Society Biological Sciences, Series B 276(1660), 1313–1322.Google ScholarPubMed
Sankaran, K.V., Murphy, S.T. & Sreenivasan, M.A. (2003) When good trees turn bad: the unintended spread of introduced plantation tree species in India. pp. 39–107 in Proceedings Asia-Pacific Forest Invasive Species Conference. 17–23 August 2003, Kunming, China.Google Scholar
Soltis, D.E. (1989) Isozyme in Plant Biology. Porland, OR, Dioscorides Press.Google Scholar
Soltis, D.E., Haufler, C.H., Darrow, D.C. & Gastony, G.J. (1983) Starch gel electrophoresis of ferns: a compilation of grinding buffers, gel and electrode buffers, and staining schedules. American Fern Journal 73(1), 9–27.CrossRefGoogle Scholar
Watt, W.B. (1977) Adaptation at specific loci. I. Natural selection on phosphoglucose isomerase of Colias butterflies: biochemical and population aspects. Genetics 87(1), 177–194.CrossRefGoogle ScholarPubMed
Watt, W.B., Wheat, C.W., Meyer, E.H. & Martin, J.-F. (2003) Adaptation at specific loci. VII. Natural selection, dispersal and the diversity of molecular–functional variation patterns among butterfly species complexes (Colias: Lepidoptera, Pieridae). Molecular Ecology 12, 1265–1275.CrossRefGoogle ScholarPubMed
Wheat, C.W., Watt, W.B. & Boutwell, C.L. (2005) A reconnaissance of population genetic variation in arctic and subarctic sulfur butterflies (Colias spp.; Lepidoptera, Pieridae). Canadian Journal of Zoology-Revue Canadienne De Zoologie 83(12), 1614–1623.CrossRefGoogle Scholar
Wheat, C.W., Watt, W.B., Pollock, D.D. & Schulte, P.M. (2006) From DNA to fitness differences: Sequences and structures of adaptive variants of Colias phosphoglucose isomerase (PGI). Molecular Biology and Evolution 23(3), 499–512.CrossRefGoogle ScholarPubMed
Yamanaka, T., Tatsuki, S. & Shimada, M. (2001) Flight characteristics and dispersal patterns of fall webworm (Lepidoptera: Arctiidae) males. Environmental Entomology 30(6), 1150–1157.CrossRefGoogle Scholar
Yang, Z. & Zhang, Y. (2007) Researches on techniques for biocontrol of the fall webworm, Hyphantria cunea, a severe invasive insect pest to China. Chinese Bulletin of Entomology 44(4), 465–471.Google Scholar
Zhang, Y., Wang, Y., Xu, B. & Qu, L. (2005) Hyphantria cunea artificial breeding and propagating method and artificial feed for larva. Chinese Academy of Forestry Institute of Forest Protection. Patent 200510127628.9.Google Scholar
Zheng, C.Z., Ovaskainen, O. & Hanski, I. (2009) Modelling single nucleotide effects in phosphoglucose isomerase on dispersal in the Glanville fritillary butterfly: coupling of ecological and evolutionary dynamics. Philosophical Transactions of the Royal Society, Series B: Biological Sciences 364(1523), 1519–1532.CrossRefGoogle ScholarPubMed