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The Anopheles maculipennis complex (Diptera: Culicidae): comparison of the cuticular hydrocarbon profiles determined in adults of five Palaearctic species

Published online by Cambridge University Press:  10 July 2009

A. Phillips ,
A. Sabatini ,
P.J.M. Milligan ,
D. Boccolini ,
G. Broomfield  and
D.H. Molyneux
A. Phillips
Affiliation:
Department of Biological Sciences, University of Salford, UK
A. Sabatini
Affiliation:
Istituto Superiore di Sanità, Rome, Italy
P.J.M. Milligan
Affiliation:
Department of Biological Sciences, University of Salford, UK
D. Boccolini
Affiliation:
Istituto Superiore di Sanità, Rome, Italy
G. Broomfield
Affiliation:
Department of Biological Sciences, University of Salford, UK
D.H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, UK
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Abstract

A comparison was made between the cuticular hydrocarbons of five Palaearctic species of the Anopheles maculipennis Meigen complex; A. maculipennis sensu stricto, A. melanoon Hackett, A. messeae Falleroni, A. labranchiae Falleroni and A. atroparvus Van Thiel. Females of these species had their cuticular lipid removed and the hydrocarbons separated and quantified by gas chromatography. Discriminant analysis determined the degree of difference between the species. Wild caught adults of the complex had an average correct classification rate of 77.9%. A. atroparvus and A. labranchiae are homosequential and have no uniquely diagnostic isoenzymes, but expressed distinct hydrocarbon profiles enabling them to be separated in more than 86% of cases. Similarly, A. maculipennis sensu stricto and A. melanoon differ only by minor karyotype alterations, yet their hydrocarbon profiles could be separated with 83% correct classification. A dendrogram was drawn up, based on the hydrocarbons, using the Mahalanobis distances between species. A. maculipennis sensu stricto and A. melanoon were the two closest groups; A. messeae was next to join the cluster, followed by A. labranchiae and then A. atroparvus. These last two species were also very close to each other, but quite distant from A. maculipennis sensu stricto and A. melanoon. The species' relationships based on hydrocarbons thus reflect the tentative chromosomal phylogeny of the complex. In nature, hydrocarbon differences between species may be a device enabling the recognition of suitable mates. Studies showing that hydrocarbon dissimilarity is elevated between sympatric populations are also discussed in support of this theory.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 80 , Issue 4 , December 1990 , pp. 459 - 464
Copyright
Copyright © Cambridge University Press 1990

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References

Antony, C. & Jallon, J.M. (1982) The chemical basis for sex recognition in Drospohila melanogaster. Journal of Insect Physiology 28, 873–880.CrossRefGoogle Scholar
Barr, A.R. & Guptavanij, P. (1988) Anopheles hermsi n. sp. an unrecognised American species of the Anopheles maculipennis group (Diptera: Culicidae). Mosquito Systematics 20, 352–356.Google Scholar
Boccolini, D., Sabatini, A. & Coluzzi, M. (1986) Valore diagnostico del numero dei rami delle setole antepalmate per l'identificazione delle specie italiane del complesso Anopheles maculipennis. Annali d'Istituto Superiore di Sanità 22, 201–204.Google Scholar
Bonavita-Courgourdan, A., Clement, J.L. & Lange, C. (1987) Nestmate recognition: the role of cuticular hydrocarbons in the ant Camponotus vagus Scop. Journal of Entomological Science 22, 1–10.CrossRefGoogle Scholar
Bullini, L. & Coluzzi, M. (1978) Applied and theoretical significance of electrophoretic studies in mosquitoes (Diptera: Culicidae). Parassitologia 20, 7–21.Google ScholarPubMed
Bullini, L., Bianchi Bullini, A.P., Cianchi, R., Sabatini, A. & Coluzzi, M. (1980) Tassonomia biochimica del complesso Anopheles maculipennis. Parassitologia 22, 290–292.Google Scholar
Carlson, D.A. & Langley, P.A. (1986) Tsetse alkenes: appearance of novel sex-specific compounds as an effect of mating. Journal of Insect Physiology 32, 781–790.CrossRefGoogle Scholar
Carlson, D.A. & Service, M.W. (1979) Differentiation between species of the Anopheles gambiae Giles complex (Diptera: Culicidae) by analysis of cuticular hydrocarbons. Annals of Tropical Medicine and Parasitology 73, 589–592.CrossRefGoogle ScholarPubMed
Carlson, D.A., Nelson, D.R., Langley, P.A., Coates, T.W., Davis, T.L. & Leegwater-Van der Linden, M.E. (1974) Contact sex pheromone in the tsetse fly Glossina pallidipes (Austen): identification and synthesis. Journal of Chemical Ecology 10, 429–450.CrossRefGoogle Scholar
Cianchi, R., Sabatini, A., Bullini, L. & Coluzzi, M. (1981) Differenziazione morfologica e genetica nei complessi Anopheles maculipennis e Anopheles claviger. Parassitologia 23, 158–163.Google Scholar
Cianchi, R., Sabatini, A., Boccolini, D., Bullini, L. & Coluzzi, M. (1987) Electrophoretic evidence of reproductive isolation between sympatric populations of Anopheles melanoon and Anopheles subalpinus. p. 156in Proceedings of the Third International Congress of Malaria and Babesiosis, Annency 1987.Google Scholar
Coluzzi, M. (1970) Sibling species in Anopheles and their importance in malariology. Miscellaneous Publications of the Entomological Soceity of America 7, 63–77.Google Scholar
Corradetti, A. (1934) Ricerche sugli incroci tra le varietà di Anopheles maculipennis. Rivista Malariologica 13, 707–720.Google Scholar
Dixon, W.J. (1988) BMDP Statistical software manual (1988 software release) Vol. 1. Berkeley, CA, USA, University of California Press.Google Scholar
Emmens, R.L. (1981) Evidence for an attractant in cuticular lipids of female Lucilia cuprina (Wied.) Australian sheep blowfly. Journal of Chemical Ecology 7, 529–541.CrossRefGoogle Scholar
Frizzi, G. (1953) Etude cytogenetique d'Anopheles maculipennis en Italie. Bulletin of the World Health Organization 9, 335–344.Google Scholar
Jallon, J.M. (1984) A few chemical words exchanged by Drosophila during courtship and mating. Behaviour Genetics 14, 441–478.CrossRefGoogle ScholarPubMed
Kamhawi, S., Molyneux, D.H., Killick-Kendrick, R., Milligan, P.J.M., Phillips, A., Wilkes, A.J. & Killick-Kendrick, M. (1987) Two populations of Phlebotomus ariasi in the Cevennes focus of leishmaniasis in the south of France revealed by analysis of cuticular hydrocarbons. Medical and Veterinary Entomology 1, 97–102.CrossRefGoogle ScholarPubMed
Lockey, K.H. (1988) Lipids of the insect cuticle: origin, composition and function. Comparative Biochemistry and Physiology 89B, 595–645.Google Scholar
Mackley, J.W. & Broce, A.B. (1981) Evidence of a female sex recognition pheromone in the screwworm fly. Environmental Entomology 10, 406–408.Google Scholar
Milligan, P. J. M., Phillips, A., Broomfield, Gl, Molyneuw, D. H., Toure, Y. & Coluzzi, M. (in prep.). Cuticular hydrocarbons and species discrimination in the Anopheles gambiae complex.Google Scholar
Milligan, P.J.M., Phillips, A., Molyneux, D.H., Subbarao, S.K. & White, G.B. (1986) Differentiation of Anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components. Bulletin of Entomological Research 76, 529–537.Google Scholar
Nelson, D.R., Dillwith, J.W. & Blomquist, G.J. (1981) Cuticular hydrocarbons of the house fly Musca domestica. Insect Biochemstry 11, 187–197.CrossRefGoogle Scholar
Peschke, K. (1987) Cuticular hydrocarbons regulate mate recognition, male aggression and female choice of the rove beetle, Aleochara curtula. Journal of Chemical Ecology 13, 1993–2008.CrossRefGoogle ScholarPubMed
Phillips, A., Milligan, P.J.M., Coluzzi, M., Toure, Y., Broomfield, G. & Molyneux, D.H. (1987) Studies of the cuticular hydrocarbons of the chromosomal forms of Anopheles gambiae s.str. and A. arabiensis. p. 164in Proceedings of the Third International Congress of Malaria and Babesiosis, Annency, 1987, Abstracts.Google Scholar
Phillips, A., Milligan, P.J.M., Broomfield, G. & Molyneux, D.H. (1988) Identification of medically important Diptera by analysis of cuticular hydrocarbons. Chapter 4 in Service, M.W. (Ed.) Biosystematics of Haematophagous Insects. Oxford, Clarendon Press (Systematics Association Special Volume No. 37).Google Scholar
Scott, D., Richmond, R.C. & Carlson, D.A. (1988) Pheromones exchanged during mating: a mechanism for mate assessment in Drosophila. Animal Behaviour 36, 1164–1173.Google Scholar
White, G.B. (1978) Systematic reappraisal of the Anopheles maculipennis complex. Mosquito Systematics 10, 13–14.Google Scholar