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Transcriptomic response of citrus psyllid salivary glands to the infection of citrus Huanglongbing pathogen

Published online by Cambridge University Press:  06 March 2024

San-Tao Zhao
Affiliation:
Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China Engineering Research Center of Biotechnology for Active Substances, Ministry of Education, Chongqing Normal University, Chongqing 401331, China
Xiao-Tong Ran
Affiliation:
Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China Engineering Research Center of Biotechnology for Active Substances, Ministry of Education, Chongqing Normal University, Chongqing 401331, China
Yu-Yang Huang
Affiliation:
Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China
Wen Sang
Affiliation:
Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China
Bugenimana Eric Derrick
Affiliation:
College of Agriculture and Animal Husbandry, University of Rwanda, Kigali 999051, Rwanda
Bao-Li Qiu*
Affiliation:
Engineering Research Centre of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou 510642, China Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China Engineering Research Center of Biotechnology for Active Substances, Ministry of Education, Chongqing Normal University, Chongqing 401331, China
*
Corresponding author: Bao-Li Qiu; Email: baoliqiu@cqnu.edu.cn

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the key vector insect transmitting the Candidatus Liberibacter asiaticus (CLas) bacterium that causes the devastating citrus greening disease (Huanglongbing, HLB) worldwide. The D. citri salivary glands (SG) exhibit an important barrier against the transmission of HLB pathogen. However, knowledge on the molecular mechanism of SG defence against CLas infection is still limited. In the present study, we compared the SG transcriptomic response of CLas-free and CLas-infected D. citri using an illumine paired-end RNA sequencing. In total of 861 differentially expressed genes (DEGs) in the SG upon CLas infection, including 202 upregulated DEGs and 659 downregulated DEGs were identified. Functional annotation analysis showed that most of the DEGs were associated with cellular processes, metabolic processes, and the immune response. Gene ontology and Kyoto Encyclopaedia of Genes and Genomes enrichment analyses revealed that these DEGs were enriched in pathways involving carbohydrate metabolism, amino acid metabolism, the immune system, the digestive system, the lysosome, and endocytosis. A total of 16 DEGs were randomly selected to further validate the accuracy of RNA-Seq dataset by reverse-transcription quantitative polymerase chain reaction. This study provides substantial transcriptomic information regarding the SG of D. citri in response to CLas infection, which may shed light on the molecular interaction between D. citri and CLas, and provides new ideas for the prevention and control of citrus psyllid.

Type
Research Paper
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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