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A device for simultaneously homogenizing numbers of individual small insects for electrophoresis

Published online by Cambridge University Press:  10 July 2009

C. P. Brookes
Affiliation:
Entomology Department, Rothamsted Experimental Station, Harpenden,Herts, AL5 2JQ, UK
H. D. Loxdale
Affiliation:
Entomology Department, Rothamsted Experimental Station, Harpenden,Herts, AL5 2JQ, UK

Abstract

A simple Perspex and stainless steel device is described suitable for rapid, simultaneous homogenization of numbers (60) of small insects individually for electrophoresis. Since this device significantly decreases the time taken for preparation of samples (commonly a rate-limiting stage in electrophoretic studies), it may be of value to other workers in entomology and insect population genetics.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1985

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References

Loxdale, H. D., Castanera, P. & Brookes, C. P. (1983). Electrophoretic study of enzymes from cereal aphid populations. I. Electrophoretic techniques and staining systems for characterising isoenzymes from six species of cereal aphids (Hemiptera: Aphididae).—Bull. ent. Res. 73, 645657.CrossRefGoogle Scholar
Loxdale, H. D., Tarr, I. J., Weber, C. P., Brookes, C. P., Digby, P. G. N. & Castanera, P. (1985). Electrophoretic study of enzymes from cereal aphid populations. III. Spatial and temporal genetic variation of populations of Sitobion avenae (F.) (Hemiptera: Aphididae).Bull. ent. Ret. 75, 121141.CrossRefGoogle Scholar
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