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In vitro fermentation of four tropical browse legumes: estimation of the effect of tannins by gas production

Published online by Cambridge University Press:  27 February 2018

Rafael Rodríguez ,
Mariano Mota ,
Manuel Fondevila  and
Gabriel de la Fuente
Rafael Rodríguez
Affiliation:
Instituto de Ciencia Animal, Carretera Central km 47, 5, Apdo. Postal 24, San José de las Lajas, La Habana, Cuba
Mariano Mota
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza. Miguel Servet 177, 50013 Zaragoza (Spain). FAX 34-976 761590 E-mail: mfonde@unizar.es
Manuel Fondevila
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza. Miguel Servet 177, 50013 Zaragoza (Spain). FAX 34-976 761590 E-mail: mfonde@unizar.es
Gabriel de la Fuente
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza. Miguel Servet 177, 50013 Zaragoza (Spain). FAX 34-976 761590 E-mail: mfonde@unizar.es
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Summary

The microbial fermentation of the tropical leguminous browses Acacia cornigera (ACA), Albisia lebbekoides (ALB), Enterolobium cyclocarpum (ENT) and Leucaena leucocephala (LEU) was estimated in vitro as the final production of gas. ACA gave the greatest final (asymptotic) gas volume (D; 179 ml/g DM) and fractional rate constant (0.070). Although ENT showed the greatest rate constant (0.87), reaching half its maximum gas production in 7.8 h, it produced less gas than ACA by 9 h incubation, and did not differ from LEU by 24 h ALB gave the lowest gas production. In another trial the negative effect of tannins was estimated over 48 h as ameliorated by the addition of PEG. The effect was variable between species, the greatest being with ALB (Restriction of 0.60-0.68 of the potential gas volume due to the presence of tannins) and increasing slowly, reaching its’ maximum at 12 or 24 h with ACA (0.33) or LEU (0.16). There was no response to the addition of PEG with ENT indicating that other secondary compounds distinct from the condensed tannins were affecting microbial utilisation.

Resumen

Resumen

Se estimó in vitro la fermentación microbiana de las leguminosas arbustivas tropicales Acacia cornigera (ACA), Albisia lebbekoides (ALB), Enterolobium cyclocarpum (ENT) and Leucaena leucocephala (LEU) en términos de producción de gas. ACA registró elevados volumen máximo de gas (D; 179 ml/g MS) y tasa fraccional de fermentación (0,070). Aunque ENT mostró la mayor tasa (0,087), alcanzando la mitad de su volumen máximo tras 7,8 h, produjo menos gas que ACA a partir de las 9 h de incubación, y no difirió de LEU a partir de las 24 h. ALB registró la menor producción de gas. En otra prueba se estimó durante 48 h el efecto negativo de la concentración de taninos mediante la adición de PEG. El efecto fue variable entre especies, siendo mayor con ALB (restricción del 0,60-0,68 en el volumen potencial de gas debido a la presencia de taninos), y aumentando lentamente hasta alcanzar su máximo a las 12 o 24 h con ACA (0,33) o LEU (0,16). No hubo respuesta a la adición de PEG con ENT, indicando que otros compuestos secundarios distintos a los taninos condensados pueden afectar su utilización microbiana.

Type
Research Article
Information
BSAP Occasional Publication , Volume 34: Herbivores: Assessment of intake, digestibility and the roles of secondary compounds , 2006 , pp. 101 - 107
Copyright
Copyright © British Society of Animal Science 2006

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