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Validation of the doubly-labelled water technique in the domestic dog (Canis familiaris)

Published online by Cambridge University Press:  09 March 2007

J. R. Speakman ,
G. Perez-Camargo ,
T. McCappin ,
T. Frankel ,
P. Thomson  and
V. Legrand-Defretin
J. R. Speakman*
Affiliation:
Department of Zoology, University of Aberdeen, Aberdeen AB24 2TZ, UK
G. Perez-Camargo
Affiliation:
Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RT, UK
T. McCappin
Affiliation:
Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RT, UK
T. Frankel
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
P. Thomson
Affiliation:
Department of Zoology, University of Aberdeen, Aberdeen AB24 2TZ, UK
V. Legrand-Defretin
Affiliation:
Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RT, UK
*
*Corresponding author: Professor J. R. Speakman, fax +44 1224 272396, email speakman@aberdeen.ac.uk
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Abstract

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We validated doubly-labelled water (DLW) by comparison to indirect calorimetry and food intake–mass balance in eight Labrador dogs (24–32 kg) over 4 d. We used several alternative equations for calculating CO2 production, based on the single- and two-pool models and used two alternative methods for evaluating the elimination constants: two-sample and multiple-sampling. In all cases the DLW technique overestimated the direct estimate of CO2 production. The greatest overestimates occurred with the single-pool model. Using two samples, rather than multiple samples, to derive the elimination constants produced slightly more discrepant results. Discrepancies greatly exceeded the measured analytical precision of the DLW estimates. The higher values with DLW probably occurred because the dogs were extremely active during the 1 h in each 24 spent outside the chamber. Estimates of CO2 production from food intake–mass balance, which include this activity, produced a much closer comparison to DLW (lowest mean discrepancy 0·3 % using the observed group mean dilution space ratio and an assumption that the mass changes reflected changes in hydration for all except one animal). We recommend an equilibration time of 6 h and use of the two-pool model based on the observed population dilution space for future studies of energy demands in dogs of this body mass.

Keywords

Energy expenditureCalorimetryDoubly-labelled waterDogs
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 1 , January 2001 , pp. 75 - 87
Copyright
Copyright © The Nutrition Society 2001

References

Arnould, J, Boyd, I & Speakman, JR (1996) Measuring the body composition of antarctic fur seals (Arctocephalus gazella) by hydrogen isotope dilution Physiological Zoology 69, 93–117.CrossRefGoogle Scholar
Ballevre, O, Anantharaman-Barr, G, Gicquello, P, Piguet-Welsh, C, Thielin, A-L & Fern, E (1994) Use of the doubly-labelled water method to assess energy expenditure in free-living cats and dogs. Journal of Nutrition 124 Suppl. 12, 2594S–2600S.CrossRefGoogle Scholar
Bergmann, BM, Everson, CA, Kushida, CA, Fang, VS, Leitch, CA, Schoeller, DA, Refetoff, S & Rechtschaffen, A (1989) Sleep deprivation in the rat. V. Energy use and mediation. Sleep 12, 31–41.CrossRefGoogle Scholar
Bevan, R, Speakman, JR & Butler, PJ (1995) Validation of the heart rate and doubly labelled water techniques in tufted ducks. Functional Ecology 9, 40–48.CrossRefGoogle Scholar
Boyd, IL, Woakes, AJ, Butler, PJ, Davis, RW & Williams, TM (1995) Validation of heart rate and doubly-labelled water as measures of metabolic rate during swimming in california sea lions. Functional Ecology 9, 151–160.CrossRefGoogle Scholar
Bryant, DM & Westerterp, KP (1983) Short-term variability in energy turnover by breeding house martins) Delichon urbica: A study using doubly labelled water (D218O. Journal of Animal Ecology 52, 525–543.CrossRefGoogle Scholar
Burger, I & Johnson, J (1990) Dogs large and small: The allometry of energy requirements within a single species. Journal of Nutrition 121, S18–S21.CrossRefGoogle Scholar
Coleman, ML, Shepherd, TJ, Durham, JJ, Rouse, JE & Morre, GR (1982) Reduction of water with zinc for hydrogen isotope analysis. Analytical Chemistry 54, 993-995.CrossRefGoogle Scholar
Coward, WA, Prentice, MA, Murgatroyd, PR, Davies, HL, Cole, TJ, Sawyer, M, Goldberg, GR, Halliday, D, MacNamara, JP & AJHvan, Es (1985) Measurement of CO2 and water production rates in man using 2H, 18O labelled H2O: comparisons between calorimeter and isotope values. European Nutrition Report 5: Human Energy Metabolism: Physical Activity and Energy Expenditure Measurements in Epidemiological Research Based upon Direct and Indirect Calorimetry, pp. 126–128[AJH vanEs, editor] Es, editor]. The Hague: CIP-gegevens Koninklijke Bibliotheek.Google Scholar
Coward, WA, Ritz, P & Cole, TJ (1994) Revision of calculations in the doubly labelled water method for measurement of energy expenditure in humans. American Journal of Physiology 267, E805–E807.Google ScholarPubMed
de Wit, JC, van der Straaten, CM & Mook, WG (1980) Determination of the absolute isotopic ratio of V-SMOW and SLAP. Geostandards Newsletter 4, 33–36.CrossRefGoogle Scholar
Fancy, SG, Blanchard, JM, Holleman, DF, Kokjer, KJ & White, RG (1986) Validation of the doubly-labelled water method using a ruminant. American Journal of Physiology 251, R143–R149.Google Scholar
Gorman, ML, Mills, MG, Raath, JP & Speakman, JR (1998) High hunting costs make wild dogs vulnerable to keleptoparasitism by hyenas. Nature 391, 479–481.CrossRefGoogle Scholar
Lifson, N, Gordon, GB, Visscher, MB & Nier, AO (1949) The fate of utilised molecular oxygen and the source of the oxygen of respiratory carbon dioxide, studied with the aid of heavy oxygen. Journal of Biological Chemistry A 180, 803–811.CrossRefGoogle ScholarPubMed
Lifson, N, Gordon, GB & McClintock, R (1955) Measurement of total carbon dioxide production by means of D218O. Journal of Applied Physiology 7, 704–710.CrossRefGoogle Scholar
Lifson, N & McClintock, R (1966) Theory of use of the turnover rates of body water for measuring energy and material balance. Journal of Theoretical Biology 12, 46–74.CrossRefGoogle ScholarPubMed
Loveridge, G (1994) Provision of environmentally enriched housing for dogs. Animal Technology 45, 1–19.Google Scholar
Matthews, D & Gilker, DS (1995) Impact of 2H and 18O pool size determinations on the calculation of total energy expenditure. Obesity Research 3 Suppl. 1, 21–29.CrossRefGoogle ScholarPubMed
Midwood, AJ, Haggarty, P & McGaw, BA (1993) The doubly-labelled water method: errors due to deuterium exchange and sequestration in ruminants. American Journal of Physiology 264, R561–R567.Google Scholar
Mullen, RK (1970) Respiratory metabolism & body water turnover rates of Perognatus formosus in its natural environment. Comparative Biochemistry and Physiology 32, 259–265.CrossRefGoogle ScholarPubMed
Nagy, KA (1972) Water and electrolyte budgets of a free-living desert lizard, Sauromalus obesus. Journal of Comparative Physiology 79, 39–62.CrossRefGoogle Scholar
Nagy, KA (1983) The Doubly Labelled Water (3HH18O) Method: a Guide to its Use. UCLA Publication 12-1417. Los Angeles, CA: UCLA.Google Scholar
Nagy, KA, Sanson, GD & Jacobsen, NK (1990) Comparative field energetics of two macropod marsupials and a ruminant. Australian Wildlife Research 17, 591–599.CrossRefGoogle Scholar
Prentice, AM (1990) The Doubly Labelled Water Method for Measuring Energy Expenditure, Technical Recommendations for Use in Humans. Vienna: International Atomic Energy Agency.Google Scholar
Racette, SB, Schoeller, DA, Luke, AH, Shay, K, Hnilicka, J & Kushner, RF (1994) Relative dilution spaces of 2H- and 18O-labelled water in humans. American Journal of Physiology 30, E585-E590.Google Scholar
Roberts, SB (1989) Use of the doubly labelled water method for measurement of energy expenditure, total body water, water intake and metabolisable energy intake in humans and small animals. Canadian Journal of Physiology and Pharmacology 67, 1190–1198.CrossRefGoogle Scholar
Robinson, I (1995) The Waltham Book of Human–Animal Interaction. Benefits and Responsibilities of Pet Ownership. Londen: Pergamon.Google Scholar
Schmidt-Nielsen, K (1975) Animal Physiology: Adaptation and Environment. Cambridge: Cambridge University Press.Google Scholar
Schoeller, DA, Ravussin, E, Schutz, Y, Acheson, KJ, Baertschi, P & Jequier, E (1980) Energy expenditure by doubly labelled water: validation in humans and proposed calculation. American Journal of Physiology 250, R823–R830.Google Scholar
Schoeller, DA & Santen, E (1982) Measurement of energy expenditure in humans by doubly labelled water. Journal of Applied Physiology 53, 955–959.CrossRefGoogle Scholar
Sheng, HP & Huggins, RA (1971) Direct and indirect measurement of total body water in the growing beagle. Proceedings of the Society for Experimental Biology and Medicine 137, 1093–1099.CrossRefGoogle Scholar
Speakman, JR (1993) How should we calculate CO2 production in DLW studies of animals?. Functional Ecology 7, 746–750.Google Scholar
Speakman, JR (1995) Empirical estimation of precision in the doubly labelled water method. Obesity Research 3(Suppl. 1), 21–29.Google Scholar
Speakman, JR (1997) Doubly-labelled Water: Theory and Practice. London: Chapman & Hall.Google Scholar
Speakman, JR, Nair, KS & Goran, MI (1993) Revised equations for calculating CO2 production from doubly labelled water in humans. American Journal of Physiology 264, E912-E917.Google ScholarPubMed
Tiebout, HM & Nagy, KA (1991) Validation of the doubly-labelled water method (3HH18O) for measuring water flux and CO2 production in the tropical hummingbird. Amazilla saucerotteiPhysiological Zoology 64, 362–374.Google Scholar
Wells, JCK, Ritz, P, Davies, PSW & Coward, WA (1998) Factors affecting the H-2 to O-18 dilution space ratio in infants. Pediatric Research 43, 467–471.CrossRefGoogle Scholar
Wong, WW, Cochran, WJ, Klish, WJ, Smith, EO, Lee, LS & Klein, PD (1988) In vivo isotope fractionation factors and the measurement of deuterium- and oxygen-18-dilution spaces from plasma, urine, saliva, respiratory water vapour, and carbon dioxide. American Journal of Clinical Nutrition 47, 1–6.CrossRefGoogle Scholar