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The trans-10,cis-12 isomer of conjugated linoleic acid reduces hepatic triacylglycerol content without affecting lipogenic enzymes in hamsters

Published online by Cambridge University Press:  09 March 2007

Amaia Zabala ,
Itziar Churruca ,
M. Teresa Macarulla ,
Víctor M. Rodríguez ,
Alfredo Fernández-Quintela ,
J. Alfredo Martínez  and
María P. Portillo
Amaia Zabala
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Itziar Churruca
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
M. Teresa Macarulla
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Víctor M. Rodríguez
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Alfredo Fernández-Quintela
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
J. Alfredo Martínez
Affiliation:
Department of Physiology and Nutrition, University of Navarra, c/ Irunlarrea s/n, 31008 Pamplona, Spain
María P. Portillo*
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
*
*Corresponding author: fax + 34 945 013014, Email knppobam@vc.ehu.es
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Abstract

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Conjugated linoleic acid (CLA) refers to the positional and geometric dienoic isomers of linoleic acid. The dietary intake of CLA has been associated with changes in lipid metabolism. The aim of the present work was to assess the effects of the two main isomers of CLA on sterol regulatory element binding protein (SREBP)-1a and SREBP-1c mRNA levels, as well as on mRNA levels and the activities of several lipogenic enzymes in liver. For this purpose hamsters were fed an atherogenic diet supplemented with 5 g linoleic acid, cis-9,trans-11 or trans-10,cis-12 CLA/kg diet for 6 weeks. The trans-10,cis-12 isomer intake produced significantly greater liver weight, but also significantly decreased liver fat accumulation. No changes in mRNA levels of SREBP-1a, SREBP-1c and lipogenic enzymes, or in the activities of these enzymes, were observed. There was no effect of feeding cis-9,trans-11 CLA. These results suggest that increased fat accumulation in liver does not occur on the basis of liver enlargement produced by feeding the trans-10,cis-12 isomer of CLA in hamsters. The reduction in hepatic triacylglycerol content induced by this isomer was not attributable to changes in lipogenesis.

Keywords

Conjugated linoleic acidLipogenesisSterol regulatory element binding proteinLiverHamster
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 383 - 389
Copyright
Copyright © The Nutrition Society 2004

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