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High-fructose feeding of streptozotocin-diabetic rats is associated with increased cataract formation and increased oxidative stress in the kidney

Published online by Cambridge University Press:  09 March 2007

Rhonda C. Bell ,
John C. Carlson ,
Katrina C. Storr ,
Kelley Herbert  and
Jacob Sivak
Rhonda C. Bell*
Affiliation:
Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
John C. Carlson
Affiliation:
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
Katrina C. Storr
Affiliation:
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
Kelley Herbert
Affiliation:
School of Optometry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
Jacob Sivak
Affiliation:
School of Optometry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
*
*Corresponding author: Dr Rhonda C. Bell, present address Department of Agricultural, Food and Nutritional Science, 4-10 Ag/Forestry Centre, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5, fax +1 780 492 9130, email rhonda.bell@ualberta.ca
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Abstract

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We examined the effects of high-fructose (FR) feeding on the development of diabetic complications in the lens and the kidney of streptozotocin (STZ)-diabetic rats. Male Wistar Furth rats were treated with one of two doses of STZ (HIGH STZ, 55 mg/kg body weight; MOD STZ, 35 mg/kg body weight) or vehicle alone (SHAM) and were then assigned to a control (CNTL) or 400 g FR/kg diet for 12 weeks. At the end of the study, body weight, plasma glucose and insulin concentrations differed among STZ groups (HIGH v. MOD v. SHAM, P<0·001) but did not differ due to diet. Plasma FR concentrations were significantly higher in FR-fed v. CNTL-fed groups (P<0·0001) and in HIGH-STZ groups v. MOD-STZ and SHAM groups (P<0·0004 and P<0·0001 respectively). Focal length variability of the lens, a quantitative measure of cataract formation, was increased in the HIGH STZ, FR group compared with the HIGH STZ, CNTL group (P<0·01). The concentration of H2O2 in kidney microsomes was significantly higher in HIGH STZ, FR rats v. HIGH STZ, CNTL rats (P<0·01). Microalbuminuria was not observed in any of the groups examined, and there was no evidence of extensive histological damage in the kidney from any rats. Under conditions of severe hyperglycaemia, high FR intake promotes the development of cataracts in the lens of the eye, and results in increased concentrations of substances indicative of oxidative stress in the kidney. Although FR has been suggested as a carbohydrate source for diabetics, a high FR diet coupled with hyperglycaemia produces effects that may promote some of the complications associated with diabetes.

Keywords

FructoseCataractsOxidative damageDiabetes
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 4 , October 2000 , pp. 575 - 582
Copyright
Copyright © The Nutrition Society 2000

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