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Effects of supplementation with purified red clover (Trifolium pratense) isoflavones on plasma lipids and insulin resistance in healthy premenopausal women

Published online by Cambridge University Press:  01 August 2008

Sarah J. Blakesmith ,
Philippa M. Lyons–Wall ,
Caroline George ,
George E. Joannou ,
Peter Petocz  and
Samir Samman
Sarah J. Blakesmith
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Australia
Philippa M. Lyons–Wall
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Australia
Caroline George
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Australia
George E. Joannou
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Australia
Peter Petocz
Affiliation:
School of Mathematical Sciences, University of Technology, Australia
Samir Samman*
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Australia
*
*Corresponding Author: Associate Professor Samir Samman, fax +61 2 93516022, email s.samman@mmb.usyd.edu.au
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Abstract

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Consumption of isoflavone-rich soyabean protein is reported to reduce total and LDL-cholesterol, but the specific components responsible are undetermined. In a previous crossover trial we showed that purified isoflavones, derived from red clover (Trifolium pratense), raised HDL3-cholesterol in premenopausal women; however, these findings were inconclusive due to period and carryover effects. In an attempt to overcome this problem, we utilised a parallel study designed to re-examine the effects of purified isoflavones on plasma lipoproteins and markers of insulin resistance in premenopausal women. Twenty-five healthy premenopausal women participated in a double-blind, randomised, parallel study. The treatment group (n 12) consumed a placebo for the first menstrual cycle and an isoflavone supplement (86 mg/d, derived from red clover) for three cycles, while the placebo group (n 13) consumed a placebo supplement for four menstrual cycles. Blood samples were collected weekly during cycles 1, 3 and 4. Supplementation with isoflavones resulted in a 15-fold increase in urinary isoflavone excretion (P<0·0001). There were no significant effects on total cholesterol, LDL- and HDL-cholesterol, HDL subfractions, triacylglycerol, lipoprotein(a), glucose or insulin concentrations. Our present results indicate that purified isoflavones derived from red clover have no effect on cholesterol homeostasis or insulin resistance in premenopausal women, a group which is at low risk of CHD.

Keywords

IsoflavonesLipidsPremenopausal women
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 4 , April 2003 , pp. 467 - 474
Copyright
Copyright © The Nutrition Society 2003

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