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The effects of obesity-associated insulin resistance on mRNA expression of peroxisome proliferator-activated receptor-γ target genes, in dogs

Published online by Cambridge University Press:  01 September 2007

Constance Gayet
Affiliation:
Nutrition and Endocrinology Unit, National Veterinary School, B.P. 40706, F 44307 Nantes Cedex 3, France
Veronique Leray
Affiliation:
Nutrition and Endocrinology Unit, National Veterinary School, B.P. 40706, F 44307 Nantes Cedex 3, France
Masayuki Saito
Affiliation:
Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
Brigitte Siliart
Affiliation:
Nutrition and Endocrinology Unit, National Veterinary School, B.P. 40706, F 44307 Nantes Cedex 3, France
Patrick Nguyen*
Affiliation:
Nutrition and Endocrinology Unit, National Veterinary School, B.P. 40706, F 44307 Nantes Cedex 3, France
*
*Corresponding author: Prof. Patrick Nguyen, fax +33 240 687 746, email pnguyen@vet-nantes.fr
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Abstract

Visceral adipose tissue and skeletal muscle have central roles in determining whole-body insulin sensitivity. The peroxisome proliferator-activated receptor-γ (PPARγ) is a potential mediator of insulin sensitivity. It can directly modulate the expression of genes that are involved in glucose and lipid metabolism, including GLUT4, lipoprotein lipase (LPL) and adipocytokines (leptin and adiponectin). In this study, we aimed to determine the effects of obesity-associated insulin resistance on mRNA expression of PPARγ and its target genes. Dogs were studied when they were lean and at the end of an overfeeding period when they had reached a steady obese state. The use of a sensitive, real-time PCR assay allowed a relative quantification of mRNA expression for PPARγ, LPL, GLUT4, leptin and adiponectin, in adipose tissue and skeletal muscle. In visceral adipose tissue and/or skeletal muscle, mRNA expression of PPARγ, LPL and GLUT4 were at least 2-fold less in obese and insulin-resistant dogs compared with the same animals when they were lean and insulin-sensitive. The mRNA expression and plasma concentration of leptin was increased, whereas the plasma level and mRNA expression of adiponectin was decreased, by obesity. In adipose tissue, PPARγ expression was correlated with leptin and adiponectin. These findings, in an original model of obesity induced by a prolonged period of overfeeding, showed that insulin resistance is associated with a decrease in PPARγ mRNA expression that could dysregulate expression of several genes involved in glucose and lipid metabolism.

Type
Full Papers
Copyright
Copyright © The Authors 2007

Obesity-induced insulin resistance is associated with metabolic disorders and profound changes in glucose and lipid homeostasis. Insulin resistance is characterised by a decrease in the uptake of glucose, especially by insulin target tissues (including adipose tissue and skeletal muscle), and is accompanied by abnormal production of adipocytokines.

Peroxisome proliferator-activated receptor (PPARγ) may contribute to increased insulin sensitivity in muscle and adipose tissue by some as yet undefined molecular pathways. PPARγ promotes adipocyte differentiation, resulting in an increased number of small adipocytes more sensitive to insulinReference Okuno, Tamemoto and Tobe1, whilst in mature adipocytes, PPARγ plays a key role in lipid and glucose homeostasis through co-ordinate effects on gene transcription.

Most of its target genes encode proteins involved in the trapping, release and storage of fatty acids, such as LPLReference Schoonjans, Peinado-Onsurbe, Lefebvre, Heyman, Briggs, Deeb, Staels and Auwerx2, leptinReference Cohen, Novick and Rubinstein3, TNFαReference Miles, Romeo, Higo, Cohen, Rafaat and Olefsky4 and adiponectinReference Maeda, Takahashi and Funahashi5, Reference Yamauchi, Kamon and Waki6, and in proteins directly involved in glucose uptake such as the insulin-dependent glucose transporter GLUT4Reference Dana, Hoener, Bilakovics, Crombie, Ogilvie, Kauffman, Mukherjee and Paterniti7, Reference Wu, Xie, Morrison, Bucher and Farmer8.

In both rodents and man the majority of PPARγ is expressed in adipose tissue, and it is expressed at much lower levels in skeletal muscle. Whilst the role of PPARγ in insulin resistance and obesity is evident, the effects of these diseases on its own expression are still unclear. In obese and type 2 diabetic subjects, it has been reported that PPARγ gene expression was increased in skeletal muscleReference Park, Ciaraldi, Abrams-Carter, Mudaliar, Nikoulina and Henry9, and not altered in adipose tissueReference Rieusset, Andreelli, Auboeuf, Roques, Vallier, Riou, Auwerx, Laville and Vidal10. In contrast, it has been demonstrated that expression of PPARγ mRNA was increased in the adipose tissue of obese subjects, but not changed in the muscle of lean, obese or diabetic subjectsReference Vidal-Puig, Considine, Jimenez-Linan, Werman, Pories, Caro and Flier11. Reports that people with dominant negative mutations in the PPARγ gene suffer from severe insulin resistance suggest that PPARγ expression may be decreased in the insulin-resistant stateReference Barroso, Gurnell and Crowley12.

For many years, dogs have been used as a model of the human metabolic syndromeReference Bergman, Van Citters, Mittelman, Dea, Hamilton-Wessler, Kim and Ellmerer13. Following sequencing of the dog genome, the high degree of homology between canine and human genesReference Kirkness, Bafna and Halpern14 provides further support for the validity of this model.

In the present study, we determined the effects of obesity and insulin resistance on the expression of PPARγ and some of its target genes (including LPL, GLUT4, adiponectin, leptin, TNFα) in the skeletal muscle and/or visceral adipose tissue of dogs following prolonged overfeeding. Plasma concentrations of insulin, TAG, leptin and adiponectin were also determined.

Materials and methods

Animals

Thirteen healthy, adult, neutered, female dogs (beagles) participated in the study. Among the thirteen dogs, seven (initial body weight (BW) 11·1 (sem 0·8) kg; age 21·6 (sem 1·2) months) were used for the entire study, forming their own control and being assessed both before and after the overfeeding period. The other six dogs (initial BW 8·5 (sem 0·4) kg; age 37·6 (sem 2·0) months) were used as a control group for the study of PPARγ mRNA expression in subcutaneous adipose tissue. The dogs were housed at the National Veterinary School (Nantes, France) according to the animal welfare regulations of the French Ministry of Agriculture and Fisheries. The experimental protocols adhered to European Union guidelines and were approved by the Animal Use and Care Advisory Committee of Nantes (France).

Experimental design

In order to promote obesity and insulin resistance, seven dogs were fed a high-fat diet (Table 1), with a food allowance of 1·5 times the National Research Council recommendation for canine maintenance, i.e. 816 kJ metabolizable energy/kg BW0·75, based on initial BW. The remaining six dogs were fed a control diet at the National Research Council recommendation for maintenance (544 kJ metabolizable energy/kg BW0·75; Table 1). Individual food intakes were recorded daily and BW were recorded weekly. The dogs were considered to be obese when they had gained more than 25 % of their initial BW. An obese steady state was considered to have been achieved when BW had not changed for at least 8 weeks. Dogs were studied before weight gain and when they had reached the obese steady state.

Table 1 Nutrient profile, in the commercially available high-fat diet used to promote obesity (n 7) and the control used to maintain body weight (n 6)

High-fat diet contained rice, poultry meal, animal fat, beet pulp, poultry protein isolate, minerals, pea fibre, Torula yeast, soya oil, fish oil, minerals, trace elements, vitamins.

Control diet contained barley, poultry meal, pea fibre, animal fat, beet pulp, poultry protein isolate, Torula yeast, soya oil, minerals, fish oil, minerals, trace elements, lysine, vitamins, methionine.

Insulin sensitivity was assessed using the euglycaemic hyper-insulinaemic glucose clamp technique (as previously describedReference Bailhache, Ouguerram, Gayet, Krempf, Siliart, Magot and Nguyen15), prior to weight gain and after achieving an obese steady state. Blood samples were collected monthly following an overnight fast. For TAG, leptin and adiponectin analyses, blood samples (10 ml) were taken from the jugular vein into EDTA tubes (23·5 μmol/tube). The blood was immediately centrifuged at 4300 g for 10 min and plasma aliquots were frozen at − 20°C until analysis.

Biopsies of visceral and subcutaneous adipose tissue and skeletal muscle were obtained under anaesthesia following an overnight fast, 1 week after the euglycaemic glucose clamp. Skeletal muscle biopsies were removed from a rear thigh and the adipose tissue samples were mesenteric. Approximately 100 mg of each tissue were taken, and cleaned in saline before 1 ml TRIzol reagent (Gibco BRL, Grand Island, NY, USA) was added and the tissue was immediately frozen in liquid nitrogen.

Chemical analysis

Rapid determination of blood glucose during clamping was achieved using the glucose oxidase method (Glucotrend® Plus; Roche Diagnostics, Mannheim, Germany). Plasma immunoreactive insulin concentrations (RIA Insik-5; DiaSorin, Saluggia, Italy) and plasma immunoreactive adiponectin concentrations (RIA kit; LINCO Research Inc., St Charles, MO, USA) were measured using commercial RIA kits. Plasma TAG concentrations were analysed using enzymatic methods (TG C Wako; Oxoid, Dardilly, France) whilst plasma leptin was quantitated by ELISAReference Iwase, Kimura, Komagome, Sasaki, Ishioka, Honjoh and Saito16.

RNA extraction and analysis

Total RNA was extracted from adipose tissue and muscle using TRIzol reagent according to the manufacturer's instructions (Gibco BRL). After extraction, RNA samples were treated with ribonuclease-free deoxyribonuclease (Promega Corp., Madison, WI, USA). RNA pellets were dissolved in water. Total RNA concentration was quantified by spectrophotometric absorbance measurement at 260 nm. The 260-to-280 nm absorption ratio of all preparations was between 1·8 and 2·0. RNA integrity was assessed by gel electrophoresis using agarose-ethidium bromide gel.

Reverse transcription and real-time PCR analysis

Reverse transcription was carried out in a reaction volume of 20 μl containing 1 μg total RNA, 0·5 μg/μl pdN6 primer (Pharmacia, Saclay, Orsay Cedex, France) and Superscript II Moloney leukaemia virus RT (according to the manufacturer's instructions; Life Technologies, Cergy-Pontoise, France).

Quantitative real-time PCR was conducted with a Rotorgene 2000 (Ozyme, Saint-Quentin-en-Yvelines, France) in a 20 μl mixture containing 1x SYBR Green (Roche Diagnostic, Meylan, France), 0·25 mm-dNTP, 0·5 mm of each primer, 2 U Taq Titanium DNA polymerase (Ozyme) and 2 μl cDNA mixture of each sample.

The sense/antisense primers (Genosys, Pampisford, UK) were designed using GeneJockey Software (Biosoft, Ferguson, MO, USA). The specificity of PCR primers and the annealing temperature were tested under normal PCR conditions with a temperature gradient (55–70°C) as annealing (Table 2).

Table 2 Sense/antisense primers used for proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), GLUT4, leptin, adiponectin, TNFα and GAPDH relative quantification, and annealing temperatures determined for each PCR primer

The real-time PCR conditions were 95°C for 300 s (to activate the hot-start enzyme), followed by: (1) thirty-five cycles of 95°C for 5 s, 63°C for 10 s, 72°C for 10 s and 83°C for 15 s for PPARγ; (2) forty cycles of 95°C for 5 s, 62°C for 15 s, 72°C for 15 s and 86°C for 15 s for the LPL; (3) forty cycles of 95°C for 5 s, 65°C for 10 s, 72°C for 10 s and 87°C for 15 s for leptin; (4) forty-five cycles of 95°C for 5 s, 70°C for 15 s, 72°C for 15 s and 85°C for 15 s for GLUT4; (5) forty cycles of 95°C for 5 s, 69°C for 15 s, 72°C for 15 s and 86°C for 15 s for adiponectin; (6) forty cycles of 95°C for 5 s, 70°C for 15 s, 72°C for 15 s and 81°C for 15 s for TNFα; or (7) thirty cycles of 95°C for 5 s, 69°C for 15 s, 72°C for 15 s and 86°C for 15 s for the GAPDH.

The fluorescence cycle threshold (Ct) obtained was used to quantitate the relative amount of gene expression. Relative transcript levels were calculated as , where x-fold is the difference in the amount of starting cDNA between two samples, . GAPDH, as a housekeeping gene (the level of mRNA GAPDH is not significantly different between the two groups) was used as a reference for initial RNA loading. Time IR was designated as the time-point when the dogs were obese and insulin-resistant. Time 0 represents the one-fold expression of the target gene normalized to GAPDH when the same dogs were lean and insulin-sensitive. Ct target =  average Ct for target gene and Ct GAPDH =  average Ct for GAPDHReference Livak and Schmittgen17. The level of expression in the lean, insulin-sensitive state was arbitrarily set at 100 %.

Statistical analysis

Data are means with their standard errors. Changes and correlations were analysed statistically using non-parametric tests (Statview; Abacus Concepts Inc., Berkeley, CA, USA): Spearman test for correlations, Wilcoxon paired tests for adipose tissue and plasma parameter comparisons between the normal and obese state, and a Welch modified two-sample t test for skeletal muscle tissue values. P < 0·05 was considered significant.

Results

Weight gain and insulin sensitivity

Obese steady state was reached when BW was approximately 140 % of initial BW (from 11·1 (sem 0·8) to 16·1 (sem 1·6) kg). The period of BW gain was 55 (sem 2) weeks, the rate of BW gain 0·66 % per week, and mean energy intake 1·38 (sem 0·08) times the National Research Council recommendation for maintenance, based on initial BW.

The plasma glucose and insulin responses of the dogs during the euglycaemic hyperinsulinaemic clamps are shown in Table 3. Basal glucose concentrations were similar and stable in the dogs in both normal and obese states. Similarly, basal insulin concentrations did not differ between obese and normal states. In contrast, postprandial insulinaemia was significantly increased in obese dogs. During the clamp, insulin infusion elevated the plasma insulin value to a steady-state plateau, which averaged 154 μU/ml during the last 2 h. The glucose infusion rate required to maintain euglycaemia under these hyperinsulinaemic conditions was lower in obese dogs than when in their normal state (P < 0·05). Overall, body insulin sensitivity was therefore decreased (by approximately 30 %) by obesity.

Table 3 Body weight, plasma concentrations of TAG, leptin, adiponectin, postprandial insulinaemia and parameters of hyperinsulinaemic euglycaemic clamp in dogs before and after weight gain leading to obesity (n 7)(Mean values with their standard errors)

Mean values were significantly different from those of the lean state: *P < 0·05.

Increase in TAG and leptin concentrations, and decrease in adiponectin

Plasma TAG concentrations (P < 0·05 compared with normal state) and plasma leptin concentrations (P < 0·05 compared with normal state) were significantly increased in obese dogs, whilst plasma adiponectin concentrations were significantly decreased in obese animals (P < 0·05 compared with normal state).

mRNA expression of peroxisome proliferator-activated receptor-γ, lipoprotein lipase, leptin, GLUT4, TNFα and adiponectin in visceral adipose tissue

The mRNA expression of PPARγ and its target genes in visceral adipose tissue, which was obtained in all seven dogs in both the obese and normal states, is shown in Fig. 1. In visceral adipose tissue, LPL, GLUT4, PPARγ and adiponectin mRNA expression was approximately 2-fold less in the dogs when obese and insulin-resistant, compared with their lean and insulin-sensitive state. In contrast, mRNA expression of leptin in visceral adipose tissue was more than 8-fold higher in the dogs when obese and insulin-resistant, compared with their lean and insulin-sensitive state. mRNA expression of TNFα was increased by obesity, but this failed to reach significance (481 (sem 280) and 100 (sem 9) %).

Fig. 1 Relative peroxisome proliferator-activated receptor-γ (PPARγ; A), TNFα (B), lipoprotein lipase (LPL; C), leptin (D), GLUT4 (E) and adiponectin (F) mRNA expression in visceral adipose tissue in obese/insulin-resistant dogs (■) as compared with their lean/insulin-sensitive state (□; n 7). The level of expression in the lean state was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean values were significantly different from those of the lean state: *P < 0·05.

mRNA expression of peroxisome proliferator-activated receptor-γ in subcutaneous adipose tissue

The mRNA expression of PPARγ in subcutaneous adipose tissue (seven obese dogs, compared with six different lean control dogs) is shown in Fig. 2. Obesity decreased PPARγ mRNA in subcutaneous adipose tissue, but this failed to reach significance (38 (sem 13) and 100 (sem 36) %).

Fig. 2 Relative peroxisome proliferator-activated receptor-γ (PPARγ) mRNA expression in the subcutaneous adipose tissue of obese/insulin-resistant dogs (■; n 7) compared with lean, insulin-sensitive control dogs (□; n 6). The level of expression in the control group was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean value was not significantly different from that of the control group.

mRNA expression of peroxisome proliferator-activated receptor-γ, lipoprotein lipase and GLUT4 in skeletal muscle

The mRNA expression of LPL, PPARγ and GLUT4 in skeletal muscle, which was obtained from four dogs in both the obese and lean states, is shown in Fig. 3. In skeletal muscle, LPL mRNA expression was approximately 3-fold less in the dogs when obese and insulin-resistant, compared with their lean and insulin-sensitive state. PPARγ and GLUT4 mRNA expression was at least 2-fold less in the dogs when obese and insulin-resistant, compared with their lean and insulin-resistant state.

Fig. 3 Relative peroxisome proliferator-activated receptor-γ (PPARγ; A), lipoprotein lipase (LPL; B) and GLUT4 (C) mRNA expression in the skeletal muscle of obese/insulin-resistant dogs (■) as compared with their lean/insulin-sensitive state (□; n 4). The level of expression in the lean state was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean values were significantly different from those of the lean state: *P < 0·05.

Correlations between mRNA expression of peroxisome proliferator-activated receptor-γ and lipoprotein lipase, adiponectin, GLUT4 and leptin in visceral adipose tissue in obese and insulin-resistant dogs

In visceral adipose tissue, there was a positive correlation between PPARγ and leptin mRNA expression (r 0·830, P < 0·05), and between PPARγ and adiponectin mRNA expression (r 0·839, P < 0·05). There was no significant correlation between PPARγ and GLUT4 or LPL mRNA expression.

Discussion

PPARγ increases insulin sensitivity and the utilization of glucose in peripheral tissue by increasing the expression of GLUT4 in target cellsReference Young, Cawthorne, Coyle, Holder, Holman, Kozka, Kirkham, Lister and Smith18. PPARγ also stimulates the storage and uptake of lipids, by increasing the expression of LPL and regulating the expression of cytokines such as leptin and adiponectin.

Several studies have shown that insulin can stimulateReference Zhang, Berger, Zhou, Elbrecht, Biswas, White-Carrington, Szalkowski and Moller19 or inhibitReference Hu, Kim, Sarraf and Spiegelman20 PPARγ expression. Alterations in the expression of PPARγ might participate in the molecular mechanism of diseases with altered lipid homeostasis and insulin resistance, but the relationship between obesity and possible alterations in the expression of PPARγ has not been clearly established.

In the present study, obesity was induced with a high-fat diet. In practice, in both man and domestic animals, obesity is more frequently caused by excessive consumption of a high-carbohydrate diet. Previously described dog models of insulin resistance have used hyper-energetic diets rich in carbohydrate (fructoseReference Pamies-Andreu, Fiksen-Olsen, Rizza and Romero21) or fatReference Rocchini, Marker and Cervenka22, or a normocaloric diet slightly enriched in fatReference Kim, Ellmerer, Van Citters and Bergman23. Numerous studies have shown that excess adiposity is clearly the most important nutrition-related factor determining insulin resistanceReference McAuley and Mann24.

The present data show that obesity induced a decrease in insulin sensitivity (30 %), hyper-triglyceridaemia, hyper-leptinaemia, a decrease in plasma adiponectin level and changes in the mRNA expression of PPARγ, GLUT4, LPL, adiponectin and leptin in skeletal muscle and/or visceral adipose tissue. It was shown in previous studies that insulin resistance and changes in PPARγ mRNA expression can be related to ageing. However, in the present study, three dogs were fed with a regular control diet for the same period as our experimental dogs. No changes were observed in insulin sensitivity or any plasma parameters (data not shown). Furthermore, the dogs were all young. In agreement with the literatureReference Peterson, Befroy, Dufour, Dziura, Ariayan, Rothman, DiPietro, Cline and Shulman25, the present data suggest that the modifications observed in our obese and insulin-resistant dogs were related to obesity and were not due to ageing. The insulin sensitivity of the obese dogs in the present study was significantly decreased, and we found, as has been reported in other speciesReference Mingrone, Rosa, Di Rocco, Manco, Capristo, Castagneto, Vettor, Gasbarrini and Greco26, a decrease in GLUT4 mRNA expression in both insulin target tissues (visceral adipose tissue and skeletal muscle). In parallel, we observed a decrease in PPARγ mRNA expression in the visceral adipose tissue of obese and insulin-resistant animals. Previous findings in miceReference Kageyama, Hirano, Okada, Ebara, Kageyama, Murakami, Shioda and Adachi27 and manReference Montague, Prins, Sanders, Zhang, Sewter, Digby, Byrne and O'Rahilly28 showed also a negative correlation between BMI and PPARγ mRNA expression. However, no change in basal PPARγ mRNA levels in the subcutaneous adipose tissue of obese and type 2 diabetic patients has been reportedReference Rieusset, Andreelli, Auboeuf, Roques, Vallier, Riou, Auwerx, Laville and Vidal10. Such differences could be due to variability in the stage and characteristics of insulin resistance. The obese dogs in the present study were in a steady state, with stable BW and insulin resistance, but without fasted hyperinsulinaemia and hyperglycaemia.

There is also likely to be variability depending on the source of the tissue studied. It is well-established that increased visceral adiposity is typically seen in people with insulin-resistance syndrome or type 2 diabetes, and that it plays a more prominent pathogenic role than subcutaneous adiposityReference Fujioka, Matsuzawa, Tokunaga and Tarui29, Reference Kelley, Thaete, Troost, Huwe and Goodpaster30. Indeed, in the subcutaneous adipose tissue of the dogs in the present study, we did not observe any significant modulation of PPARγ expression. In skeletal muscle, we found a decrease in PPARγ mRNA expression. This is in agreement with the fact that mice with a muscle-specific deletion of PPARγ developed excess adiposity as well as whole-body insulin resistanceReference Norris, Chen and Fisher31. It has been concluded that PPARγ in muscle could play a role in the regulation of whole-body lipid storage, via alterations in the regulation of genes involved in muscle lipid metabolism. These findings are consistent with our present results. Moreover, the present data support the finding that in vivo activation of PPARγ by treatment of insulin-resistant rodents with thiazolidinediones corrects impaired muscle insulin actionReference Zierath, Ryder and Doebber32.

Our obese dogs exhibited hyper-triglyceridaemia, and in both visceral adipose tissue and skeletal muscle we observed a 2-fold decrease in the mRNA expression of LPL, a major enzyme for TAG hydrolysis that regulates plasma TAG concentrationReference Eckel33. This suggests insulin resistance-induced abnormalities in LPL function, and is in agreement with data previously reported from our laboratoryReference Bailhache, Nguyen, Krempf, Siliart, Magot and Ouguerram34. In the literature, the reported changes in LPL expression precipitated by insulin resistance are contradictoryReference Kageyama, Hirano, Okada, Ebara, Kageyama, Murakami, Shioda and Adachi27, Reference Preiss-Landl, Zimmermann, Hammerle and Zechner35. The decreased LPL mRNA expression in skeletal muscle and adipose tissue in the present study is consistent with data reported in manReference Boucher, Ducluzeau, Davelu, Andreelli, Vallier, Riou, Laville and Vidal36 and miceReference Kageyama, Hirano, Okada, Ebara, Kageyama, Murakami, Shioda and Adachi27. Furthermore, it has been showed that LPL mRNA levels decreased following TNFα perfusion via a transient decrease in PPARγ expression in white adipose tissueReference Hill, Young, McCurdy and Gimble37.

The increased TNFα mRNA expression in the obese and insulin-resistant dogs in the present study was not significant. This could be due to a high standard deviation caused by the great variability in the degree of obesity achieved by the dogs. Furthermore, in a previous study carried out in our laboratory, we found a significant increase in plasma TNFα levels in obese and insulin-resistant dogs compared with lean dogsReference Gayet, Bailhache, Dumon, Martin, Siliart and Nguyen38.

PPARγ is likely to modulate the expression of many other genes, especially the adipocytokines, leptin and adiponectin. In the present study, the hyper-leptinaemia observed in obese and insulin-resistant dogs was associated with a considerable increase in leptin mRNA expression: these data are consistent with an increase in fat mass and with the inhibitory effect of activated-PPARγ on leptin gene expressionReference De Vos, Lefebvre, Miller, Guerre-Millo, Wong, Saladin, Hamann, Staels, Briggs and Auwerx39, Reference Kallen and Lazar40, which could result in the over-expression of the cytokine when PPARγ mRNA expression is altered. The plasma level of adiponectin and its mRNA expression in visceral adipose tissue were significantly decreased in obese and insulin-resistant dogs. The present findings support those obtained in manReference Maeda, Takahashi and Funahashi5 and in rodent models of obesityReference Arita, Kihara and Ouchi41 where PPARγ has been shown to be a potential regulator of adiponectinReference Yamauchi, Kamon and Waki6. The present therefore suggest that in dogs too, alteration in plasma adiponectin may be due to decreased adiponectin expression following the reduction in PPARγ expression.

In the present study, the mRNA expression of PPARγ in the visceral adipose tissue of obese and insulin-resistant dogs was correlated with the mRNA expression of adiponectin, and leptin, strengthening the hypothesis that an alteration in PPARγ mRNA expression may have a role in insulin resistance. In human skeletal muscle, a positive correlation between expression of PPARγ and LPL mRNA has been describedReference Lapsys, Kriketos, Lim-Fraser, Poynten, Lowy, Furler, Chisholm and Cooney42. In canine skeletal muscle we did not find any correlation between PPARγ mRNA expression and any of its target genes: the observed changes in mRNA levels were significant, and we used a highly sensitive and reproducible real-time PCR assay, however, the sample size for analyses on this tissue was small (four dogs).

We used a dog model of steady-state obesity (compared with the initial lean state) following a long-term overfeeding period to study the effects of obesity-associated insulin resistance on the mRNA expression of PPARγ and its target genes. Previous studies have shown a similar but shorter-term model of the insulin-resistant dog to be useful for studying human insulin resistance-associated dyslipidaemiaReference Bailhache, Nguyen, Krempf, Siliart, Magot and Ouguerram34. In addition to the changes in lipoprotein metabolism described previously, we observed several other canine parameters that are modulated in the same way as during human insulin-resistance disease. The dogs in the present study developed hyper-triglyceridaemia, suggesting alterations in LPL functionality. Indeed, in insulin-resistant animals, mRNA LPL was decreased in both adipose tissue and skeletal muscle, whilst plasma and mRNA expression of adipocyte-derived cytokines was increased (leptin) and decreased (adiponectin). The mRNA expression of the insulin-dependent glucose transporter GLUT4 was also reduced in skeletal muscle and visceral adipose tissue. We observed consistent changes in mRNA PPARγ expression and furthermore, there was a correlation between mRNA expression of PPARγ and adiponectin and leptin in adipose tissue. In the present study, the expression of PPARγ and its target genes was studied in two insulin target tissues: skeletal muscle (the major site for insulin-stimulated glucose uptake) and visceral adipose tissue (the major adipose compartment associated with insulin resistance). In contrast, most of the data in the literature were obtained solely on subcutaneous adipose tissue.

In summary, all the present findings suggest that, in dogs, obesity-associated insulin resistance alters PPARγ mRNA expression and PPARγ-responsive genes that influence metabolism. Considering the notion that insulin resistance is associated with a decrease in PPARγ mRNA expression, in addition to a decrease in its activity, may allow new approaches to the study of this metabolic disorder to be formulated.

Acknowledgements

We thank Samuel Ninet and Gérald Pondevie for taking care of our animals. We also thank Philippe Bleis for technical support during the biopsies.

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Figure 0

Table 1 Nutrient profile, in the commercially available high-fat diet used to promote obesity (n 7) and the control used to maintain body weight (n 6)

Figure 1

Table 2 Sense/antisense primers used for proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), GLUT4, leptin, adiponectin, TNFα and GAPDH relative quantification, and annealing temperatures determined for each PCR primer

Figure 2

Table 3 Body weight, plasma concentrations of TAG, leptin, adiponectin, postprandial insulinaemia and parameters of hyperinsulinaemic euglycaemic clamp in dogs before and after weight gain leading to obesity (n 7)(Mean values with their standard errors)

Figure 3

Fig. 1 Relative peroxisome proliferator-activated receptor-γ (PPARγ; A), TNFα (B), lipoprotein lipase (LPL; C), leptin (D), GLUT4 (E) and adiponectin (F) mRNA expression in visceral adipose tissue in obese/insulin-resistant dogs (■) as compared with their lean/insulin-sensitive state (□; n 7). The level of expression in the lean state was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean values were significantly different from those of the lean state: *P < 0·05.

Figure 4

Fig. 2 Relative peroxisome proliferator-activated receptor-γ (PPARγ) mRNA expression in the subcutaneous adipose tissue of obese/insulin-resistant dogs (■; n 7) compared with lean, insulin-sensitive control dogs (□; n 6). The level of expression in the control group was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean value was not significantly different from that of the control group.

Figure 5

Fig. 3 Relative peroxisome proliferator-activated receptor-γ (PPARγ; A), lipoprotein lipase (LPL; B) and GLUT4 (C) mRNA expression in the skeletal muscle of obese/insulin-resistant dogs (■) as compared with their lean/insulin-sensitive state (□; n 4). The level of expression in the lean state was arbitrarily set at 100 %. Values are means with their standard errors depicted by vertical bars. Mean values were significantly different from those of the lean state: *P < 0·05.