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Effect of selenium deficiency on hepatic lipid and lipoprotein metabolism in the rat

Published online by Cambridge University Press:  09 March 2007

F. Nassir ,
C. Moundras ,
D. Bayle ,
C. Sérougne ,
E. Gueux ,
E. Rock ,
Y. Rayssiguier  and
A. Mazur
F. Nassir
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
C. Moundras
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
D. Bayle
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
C. Sérougne
Affiliation:
Laboratoire de Physiologie de la Nutrition, Université Paris Sud, 91405 Orsay, France
E. Gueux
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
E. Rock
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
Y. Rayssiguier
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
A. Mazur
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
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Abstract

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Since experimental Se deficiency results in a significant increase in plasma cholesterol concentration the present investigation was undertaken to assess further the influence of this deficiency on the expression of proteins involved in hepatic lipid metabolism. Se deficiency was induced by feeding weanling male Wistar rats on a deficient diet for 6 weeks. Hypercholesterolaemia associated with Se deficiency was related to increased 3-hydroxy-3-methylglutaryl-coA (HMG-CoA) reductase (EC 1.1.1.34) activity in liver microsomes as compared with control animals. Hepatic lipoprotein receptor levels (LDL-receptor and HDL-binding proteins, HB1 and HB2) were not significantly affected by Se deficiency, as assessed by immunoblotting. Plasma triacylglycerol concentrations tended to decrease in Se-deficient rats in concert with their reduced post-Triton secretion. There was no significant effect of Se deficiency on the hepatic synthesis of apolipoproteins. These results point to the need for further investigations into the mechanism related to the increased activity of HMG-CoA reductase and the enhanced cholesterogenesis in the liver of Se-deficient rats likely to result from this.

Selenium: Cholesterol: Triacylglycerol: HMG-CoA reductase

Keywords

SeleniumCholesterolTriacylglycerolHMG-CoA reductase
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 3 , September 1997 , pp. 493 - 500
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Baum, C. L., Teng, B.-B. & Davidson, N. O. (1990) Apolipoprotein B messenger RNA editing in the rat liver. Modulation by fasting and refeeding a high carbohydrate diet. Journal of Biological Chemistry 265, 19263–19270.CrossRefGoogle ScholarPubMed
Bellanger, J., Bouvier, J. C. & Lab, C. (1992) Dosage du sélénium dans le vin par spectrofluorimétrie. Application à quelques vins français (Selenium level in wine measured by spectrofluorimetry. Application to some French wines). Journal International des Sciences de la Vigne et du Vin 26, 287–293.Google Scholar
Bunce, G. E. (1993) Hypercholesterolaemia of copper deficiency is linked to glutathione metabolism and regulation of hepatic HMG CoA reductase. Nutrition Reviews 51, 305–307.CrossRefGoogle ScholarPubMed
Davis, R. A., Dluz, S. M., Leighton, J. K. & Brengaze, V A. (1989) Increased translatable mRNA and decreased lipogenesis are responsible for augmented secretion of lipid-deficient apolipoprotein E by hepatocytes from fasted rats. Journal of Biological Chemistry 264, 8970–8977.Google Scholar
Einarsson, K., Angelin, B., Ewerth, S., Niessel, K. & Bjorkhem, I. (1986) Bile acid synthesis in man: assay of hepatic microsomal cholesterol 7-alpha hydroxylase activity by isotope dilution-mass spectrometry. Journal of Lipid Research 27, 82–88.Google Scholar
Eisenberg, S. (1980) Plasma lipoprotein conversion: the origin of low density and high-density lipoproteins. Annals of the New York Academy of Sciences 348, 30–47.CrossRefGoogle ScholarPubMed
Felgines, C., Sérougne, C., Mathé, D., Mazur, A. & Lutton, C. (1994) Effect of simvastatin treatment on plasma apolipoproteins and hepatic apolipoprotein mRNA levels in the genetically hypercholesterolaemic rats (RICO). Life Sciences 54, 361–367.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–506.Google Scholar
Gavish, D., Oschry, Y. & Eisenberg, S. (1987) In vivo conversion of human HDL3 to HDL2 and apo E-rich HDL1 in the rat: effects of lipid transfer protein. Journal of Lipid Research 28, 257–267.CrossRefGoogle Scholar
Hsu, R. Y., Butterworth, P. H. W. & Porter, J. W. (1969). Pigeon liver fatty acid synthase. In Methods in Enzymology, Vol. 14, pp. 33–39 [Lowenstein, J. M., editor]. New York: Academic Press.Google Scholar
Kim, S., Chao, P. Y. & Allen, K. G. D. (1992) Inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolaemia. FASEB Journal 6, 2467–2471.CrossRefGoogle Scholar
Kovanen, P. T., Brown, M. S. & Goldstein, J. L. (1979) Increased binding of low density lipoprotein to liver membranes from rats treated with 17 α-ethinyl estradiol. Journal of Biological Chemistry 254, 11367–11373.CrossRefGoogle ScholarPubMed
Lii, C.-K. & Hendrich, S. (1993) Selenium deficiency supresses the S-glutathiolation of carbonic anhydrase III in rat hepatocytes under oxidative stress. Journal of Nutrition 123, 1480–1486.Google Scholar
Lusk, L. T., Walker, L. F., Dubien, L. H. & Getz, G. S. (1979) Isolation and characterization of high-density lipoprotein HDL1 from rat plasma by gradient centrifugation. Biochemical Journal 183, 83–90.CrossRefGoogle ScholarPubMed
Maeda, H., Ikeda, I. & Sugano, M. (1975) Behavior of the liver key lipogenesis enzymes in rat threonine imbalanced diet. Nutrition Reports International 12, 61–66.Google Scholar
Mahley, R. W. & Holcombe, K. S. (1977) Alterations of the plasma lipoproteins and apolipoproteins following cholesterol feeding in the rat. Journal of Lipid Research 18, 314–324.Google Scholar
Mathai, D., Fidge, N., Tozuka, M. & Mitcheli, A. (1990) Regulation of hepatic high density lipoprotein binding proteins after administration of simvastatin and cholestyramine to rats. Arteriosclerosis 10, 1045–1050.CrossRefGoogle ScholarPubMed
Mazur, A., Nassir, F., Gueux, E., Moundras, C., Bellanger, J., Grolier, P., Rock, E. & Rayssiguier, Y. (1996) Diets deficient in selenium and vitamin E affect plasma lipoprotein and apolipoprotein concentrations in the rat. British Journal of Nutrition 76, 899–907.Google Scholar
Mazur, A., Rémésy, C., Gueux, E., Levrat, M. A. & Demigné, C. (1990) Effects of diets rich in fermentable carbohydrates on plasma lipoprotein levels and on lipoprotein catabolism in rats. Journal of Nutrition 120, 1037–1045.CrossRefGoogle Scholar
Nassir, F., Mazur, A., Felgines, C. & Rayssiguier, Y. (1993) Age related response to dietary fructose in the rat: discrepancy in triglyceride and apo B synthesis as a possible mechanism for fatty liver induction in adult rats. Proceedings of the Society for Experimental Biology and Medicine 204, 180–183.Google Scholar
Nassir, F., Mazur, A., Gueux, E., Sérougne, C. & Rayssiguier, Y. (1994) Apolipoprotein A-I, A-IV and E synthesis in the liver of copper-deficient rats. Lipids 29, 727–729.Google Scholar
Oschry, Y. & Eisenberg, S. (1982) Rat plasma lipoproteins: re-evaluation of a lipoprotein system in an animal devoid of cholesteryl ester transfer activity. Journal of Lipid Research 23, 499–507.Google Scholar
Oster, O. & Prellwitz, W. (1990) Selenium and cardiovascular disease. Biological Trace Element Research 24, 91–103.Google Scholar
Paglia, D. E. & Valentine, W. N. (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158–169.Google ScholarPubMed
Scott, R. L., Kheshti, A., Heimberg, M., Wilcox, H. G. & Stone, W. L. (1991) The role of selenium in the secretion of very-low-density lipoprotein in the isolated perfused liver. Biochemical Journal 279, 741–745.Google Scholar
Sérougne, C., Felgines, C., Férézou, J., Hajri, T., Bertin, C. & Mazur, A. (1995) Hypercholesterolemia induced by cholesterol- or cystine-enriched diets is characterized by different plasma lipoprotein and apolipoprotein concentrations in rats. Journal of Nutrition 125, 35–41.Google Scholar
Stone, W. L. (1988) The effects of cholesterol supplementation on plasma lipoprotein-cholesterol levels in rats fed diets deficient in vitamin E and/or selenium. Nutrition Research 8, 1061–1071.Google Scholar
Stone, W. L., Scott, R. L., Stewart, E. M. & Kheshsti, A. (1994) Lipoprotein alterations in the spontaneously hypertensive rat fed diets deficient in selenium and vitamin E. Proceedings of the Society for Experimental Biology and Medicine 206, 130–137.CrossRefGoogle ScholarPubMed
Stone, W. L., Stewart, M. E., Nicholas, C. & Pavuluri, S. (1986) Effects of dietary selenium and vitamin E on plasma lipoprotein cholesterol levels in male rats. Annals of Nutrition and Metabolism 30, 94–103.CrossRefGoogle ScholarPubMed
Tozuka, M. & Fidge, N. (1989) Purification and characterization of two high-density-lipoprotein-binding proteins from rat and human liver. Biochemical Journal 261, 239–244.CrossRefGoogle ScholarPubMed
Wilce, P. & Kroone, P. A. (1992). Assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase. In Lipoprotein Analysis –– A Practical Approach, pp. 203–214 [Converse, C. A., Skinner, E. R., editors]. Oxford: Oxford University Press.CrossRefGoogle Scholar