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Calcium deficiency-induced secondary hyperparathyroidism and osteopenia are rapidly reversible with calcium supplementation in growing rabbit pups

Published online by Cambridge University Press:  08 March 2007

Meenal Mehrotra
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Sushil K. Gupta
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Krishan Kumar
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Pradeep K. Awasthi
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Manoj Dubey
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Chandra M. Pandey
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014India
Madan M. Godbole
Affiliation:
Department of Medical Endocrinology, Centre for Endocrine Sciences, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India
Corresponding
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Abstract

The reversibility of osteopenia secondary to isolated Ca deficiency (CaDef) is still not clear. We studied the effect of severe CaDef on Ca homeostasis and bone accrual in a ‘hypercalcaemic’ animal, the rabbit, during the post-weaning period and its reversibility on Ca supplementation. Male Belgian 5-week-old rabbit pups were fed CaDef diet (0·026% Ca) for 10 weeks. As compared with those fed with a normal chow diet (0·45% Ca), CaDef pups developed significant hypocalcaemia (P<0·05), hypocalciuria (urinary Ca 76 (sem 12) V 17 (sem 1) mg/l;P<0·005), hypophosphataemia (serum inorganic P 100 (sem 6) V. 65 (sem 4) mg/l; P<0·005), secondary hyperparathyroidism (SHPT) (serum intact parathyroid hormone human equivalent 18·2 (sem 1·4) V. 125·0 (sem 4·5) pg/ml; P<0·001) and elevated serum calcitriol levels (34·0 (sem 3·9) V. 91·0 (sem 1·0) pg/ml; v<0·005). Elevated urinary C-terminal telopeptide of class I collagen (P<0·005) and total serum alkaline phosphatase (P<0·005) suggested increased bone turnover. There was a significantly lower gain in bone mineral density (BMD) and bone mineral content (BMC) in the whole body and lumbar spine in vivo, and various sub-regions of the femur and tibia in vitro.Supplementation of adequate Ca (0·45% Ca) after 15 weeks on the normal diet resulted in rapid catch-up growth, and resolution of SHPT. Rapid gain in various BMD and BMC parameters continued at 30 weeks of age, and both were comparable with those in rabbits on a normal diet. We conclude that Ca deficiency-induced SHPT and poor bone accrual in growing rabbit pups are rapidly reversible with Ca supplementation. The present study indicates that early intervention may be a more appropriate window period for human nutritional corrective measures.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2006

References

Balasubramanian, K, Rajeshwari, J, Govil, YC, Agarwal, AK, Kumar, A & Bhatia, v 2003 Varying role of vitamin D in etiology of rickets in young children vs adolescents in northern India. J Trop Pediatr 49, 201206.CrossRefGoogle ScholarPubMed
Bas, S, Bas, A, Lopez, I, Estepa, JC, Rodriguez, M & Aguilera-Tejero, E 2005 Nutritional secondary hyperparathyroidism in rabbits. Domest Anim Endocrinol 28, 380390.CrossRefGoogle ScholarPubMed
Bhimma, R, Pettifor, JM, Coovadia, HM, Moodley, G & Adhikari, M 1995 Rickets in black children beyond infancy in Natal. S Afr Med J 85, 668672.Google ScholarPubMed
Bourdeau, JE, Schwer-Dymerski, DA, Stern, PH & Langman, CB 1986 Calcium and phosphorous metabolism in chronically vitamin D deficient laboratory rabbits. Miner Electrolyte Metab 12, 176185.Google ScholarPubMed
Brazy, PC, McKeown, JW, Harris, RH & Dennis, VW 1980 Comparative effects of dietary phosphate, unilateral nephrectomy and parathyroid hormone on phosphate transport by rabbit proximal tubule. Kidney Int 17, 788800.CrossRefGoogle ScholarPubMed
Buss, SL & Bourdeau, JE 1984 Calcium balance in laboratory rabbits. Miner Electrolyte Metab 10, 127132.Google ScholarPubMed
Chapin, RE & Smith, SE 1967a Calcium requirements of growing rabbits. Lab Anim Sci 26, 6771.Google Scholar
Chapin, RE & Smith, SE 1967b The calcium tolerance of growing and reproducing rabbits. Cornell Vet 57, 480491.Google ScholarPubMed
DeLucia, MC, Mitnick, ME & Carpenter, TO 2003 Nutritional rickets with normal circulating 25-hydroxyvitamin D: a call for reexamining the role of dietary calcium intake in North American infants. J Clin Endocrinol Metab 88, 35393549.CrossRefGoogle Scholar
Eyberg, C, Pettifor, JM & Moodley, G 1986 Dietary calcium intake in rural black South African Children: the relationship between calcium intake and calcium nutritional status. Hum Nutr Clin Nutr 40c, 6974.Google Scholar
Fischer, PR, Rahman, A & Cimma, JP, et al. 1999 Nutritional rickets without vitamin D deficiency in Bangladesh. J Trop Paediatr 45, 291293.CrossRefGoogle ScholarPubMed
Garabedian, M, Holick, FM, DeLuca, HF & Boyle, IT 1972 Control of 25- hydroxycholecalciferol metabolism by parathyroid glands. Proc Natl Acad Sci USA 69, 16731676.CrossRefGoogle ScholarPubMed
Gilbert, PJ, Schlondorff, D, Trizna, W & Fine, LG 1980 Renal effects of parathyroid hormone in the rabbit. Miner Electrolyte Metab 3, 291301.Google Scholar
Gilsanz, V, Roe, TF, Antunes, J, Carlson, M, Duarte, ME & Goodman, WG 1991 Effect of dietary calcium on bone density in growing rabbits. Am J Physiol 260, E471E476.Google Scholar
Gilsanz, V, Roe, TF, Gibbens, DT, Schulz, EE, Carlson, ME, Gonzalez, O, & Boechat, MI 1988 Effect of sex steroids on peak bone density of growing rabbits. Am J Physiol 255, E416E421.Google ScholarPubMed
Iwamoto, J, Takeda, T, Sato, Y & Yeh, JK 2004 Response of cortical and cancellous bones to mild calcium deficiency in young growing female rats: a bone histomorphometry study. Exp Anim 53, 347354.CrossRefGoogle ScholarPubMed
Kennedy, A 1965 The urinary excretion of calcium by normal rabbits. J Comp Pathol 75, 6974.CrossRefGoogle Scholar
Marie, PJ, Pettifor, JM, Ross, FP & Glorieux, FH 1982 Histological osteomalacia due to dietary calcium deficiency in children. N Engl J Med 307, 584588.CrossRefGoogle ScholarPubMed
Moore, T, Impey, SG, Martin, PEN & Symonds, KR 1963 Meat diets. II. Effect of the age of rats on their ability to withstand the low calcium intake induced by a diet of minced beef. J Nutr 80, 162170.CrossRefGoogle Scholar
Norris, SA, Pettifor, JM, Gray, DA & Buffenstein, R 2001 Calcium metabolism and bone mass in female rabbits during skeletal maturation: effects of dietary calcium intake. Bone 29, 6269.CrossRefGoogle ScholarPubMed
Oginni, LM, Sharp, CA, Badru, OS, Risteli, JDavie, MWJ & Worsfold, M 2003 Radiological and biochemical resolution of nutritional rickets with calcium. Arch Dis Child 88, 812817.CrossRefGoogle ScholarPubMed
Oginni, LM, Worsfold, M, Oyelami, OA, Sharp, CA, Powell, DE & Cavie, MWJ 1996 Etiology of rickets in Nigerian children. J Paediatr 128, 692694.CrossRefGoogle ScholarPubMed
Okonofua, F, Gill, DS, Alabi, ZO, Thomas, M, Bell, Jl & Dandona, P 1991 Rickets in Nigerian children: a consequence of calcium malnutrition. Metabolism 40, 209213.CrossRefGoogle ScholarPubMed
Peterson, CA, Eurell, AJ & Erdman, JW Jr 1995 Alterations in calcium intake on peak bone mass in the female rat. J Bone Miner Res 10, 8195.CrossRefGoogle ScholarPubMed
Pettifor, JM & Moodley, G 1997 Appendicular bone mass in children with high prevalence of low dietary calcium intakes. J Bone Miner Res 12, 18241832.CrossRefGoogle ScholarPubMed
Pettifor, JM, Ross, FP, Travers, R, Glorieux, FH & DeLuca, HF 1981a Dietary calcium deficiency: a syndrome associated with bone deformities and elevated serum 1, 25-dihydroxyvitamin D concentrations. Metab Bone Rel Res 2, 301305.CrossRefGoogle Scholar
Pettifor, JM, Ross, P, Moodley, G & Shuenyane, E 1979 Calcium deficiency in rural black children in South Africa – a comparison between rural and urban communities. Am J Clin Nutr 32, 24772483.CrossRefGoogle ScholarPubMed
Pettifor, JM, Ross, P, Moodley, G & Shuenyane, E 1981b The effect of dietary calcium supplementation on serum calcium, phosphorus, and alkaline phosphatase concentrations in a rural black population. Am J Clin Nutr 34, 21872191.CrossRefGoogle Scholar
Pettifor, JM, Ross, P, Wang, J, Moodley, G & Couper-Smith, JRickets in children of rural origin in South Africa: is low dietary calcium a factor?. J Pediatr 1978 92, 320324.CrossRefGoogle ScholarPubMed
Rajeshwari, J, Balasubramanian, K, Bhatia, V, Sharma, VP & Agarwal, AK 2003 Aetiology and clinical profile of osteomalacia in adolescent girls in northern India. Natl Med J India 16, 139142.Google Scholar
Sharp, CA, Oginni, LM, Worsfold, M, Oyelami, OA, Risteli, L, Risteli, J & Davie, MWJ 1997 Elevated collagen turnover in Nigerian children with calcium deficiency rickets. Calcif Tissue Int 61, 8794.CrossRefGoogle ScholarPubMed
Thomas, ML, Ibarra, MJ, Solcher, B, Wetzel, S & Simmons, DJThe effect of low dietary calcium and calcium supplementation on calcium metabolism and bone in the immature, growing rat. Bone Miner 1988 4, 7382.Google ScholarPubMed
Warren, HB, Lausen, NCC, Segre, GV, El-Hajj, G & Brown, EM 1989 Regulation of calcitrophic hormones in vivo in the New Zealand white rabbit. Endocrinology 10, 26832690.CrossRefGoogle Scholar
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