Hostname: page-component-848d4c4894-xm8r8 Total loading time: 0 Render date: 2024-06-20T02:52:10.111Z Has data issue: false hasContentIssue false
  • English
  • Français

Tolerance of bivalve mollusc hemocytes to variable oxygen availability: a mitochondrial origin?

Published online by Cambridge University Press:  04 July 2013

Ludovic Donaghy ,
Sébastien Artigaud ,
Rossana Sussarellu ,
Christophe Lambert ,
Nelly Le Goïc ,
Hélène Hégaret  and
Philippe Soudant
Get access

Abstract

Bivalve molluscs survive lack of oxygen and may present cellular characteristics allowing tolerance to oxygen deprivation. The objective of the present work was to determine in vitro responses of circulating cells (i.e., hemocytes) of Pacific oyster, Crassostrea gigas, to oxygen deprivation by analysis of mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) production. Hemocytes submitted to oxygen deprivation showed a reduced ROS production and an increased ΔΨm. Confrontation of present results with recently reported findings allows us to hypothesize the existence of an alternative mitochondrial reductase, and the involvement of mitochondrial potassium channel in maintenance of ΔΨm. Tolerance of bivalve hemocytes to variable oxygen availability appears, at least partially, related to mitochondrial specificities.

Keywords

HemocyteHypoxiaMitochondriaReactive oxygen speciesFlow cytometryOyster
Type
Perspectives
Information
Aquatic Living Resources , Volume 26 , Issue 3 , 2013 , pp. 257 - 261
Copyright
© EDP Sciences, IFREMER, IRD 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abele, E., Philip, E., Gonzalez, P.M., Puntarulo, S., 2007, Marine invertebrate mitochondria and oxidative stress. Front. Biosci. 12, 933–946. CrossRefGoogle ScholarPubMed
Bickler, P.E., Buck, L.T., 2007, Hypoxia tolerance in reptiles, amphibians, and fishes: life with variable oxygen availability. Annu. Rev. Physiol. 69, 145–170. CrossRefGoogle ScholarPubMed
Boyd, J.N., Burnett, L.E., 1999, Reactive oxygen intermediate production by oyster hemocytes exposed to hypoxia. J. Exp. Biol. 202, 3135–3143. Google ScholarPubMed
Donaghy, L., Kraffe, E., Le Goïc, N., Lambert, C., Volety, A.K., Soudant, P., 2012, Reactive oxygen species in unstimulated hemocytes of the Pacific oyster Crassostrea gigas: a mitochondrial involvement. PLoS One 7, e46594. CrossRefGoogle ScholarPubMed
Donaghy, L., Lambert, C., Choi, K.-S., Soudant, P., 2009, Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): current knowledge and future prospects. Aquaculture 297, 10–24. CrossRefGoogle Scholar
Genova, M.L., Lenaz, G., 2013, A critical appraisal of the role of respiratory supercomplexes in mitochondria. Biol. Chem. 394, 631–639. CrossRefGoogle Scholar
Hégaret, H., Wikfors, G., Soudant, P., 2003, Flow cytometric analysis of haemocytes from eastern oysters, Crassostrea virginica, subjected to a sudden temperature elevation II. Haemocyte functions: aggregation, viability, phagocytosis, and respiratory burst. J. Exp. Mar. Biol. Ecol. 293, 249–265. CrossRefGoogle Scholar
Mosca, F., Narcisi, V., Calzetta, A., Gioia, L., Finoia, M.G., Latini, M., Tiscar, P.G., 2013, Effects of high temperature and exposure to air on mussel (Mytilus galloprovincialis Lmk 1891) hemocyte phagocytosis: modulation of spreading and oxidative response. Tissue Cell 45, 198–203. CrossRefGoogle Scholar
Ramírez-Aguilar, S.J., Keuthe, M., Rocha, M., Fedyaev, V.V., Kramp, K., Gupta, K.J., Rasmusson, A.G., Schulze, W.X., van Dongen, J.T., 2011, The composition of plant mitochondrial supercomplexes changes with oxygen availability. J. Biol. Chem. 286, 43045–43053. CrossRefGoogle ScholarPubMed
Solaini, G., Baracca, A., Lenaz, G., Sgarbi, G., 2010, Hypoxia and mitochondrial oxidative metabolism. Biochim. Biophys. Acta Bioenerg. 1797, 1171–1177. CrossRefGoogle ScholarPubMed
Sussarellu, R., Fabioux, C., Camacho Sanchez, M., Le Goïc, N., Lambert, C., Soudant, P., Moraga, D., 2012, Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas. J. Exp. Mar. Biol. Ecol. 412, 87–95. CrossRefGoogle Scholar
Sussarellu, R., Dudognon, T., Fabioux, C., Soudant, P., Moraga, D., Kraffe, E., 2013, Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster Crassostrea gigas. J. Exp. Biol. 216, 1561–1569. CrossRefGoogle ScholarPubMed
Tielens, A.G.M., Rotte, C., van Hellemond, J.J., Martin, W., 2002, Mitochondria as we don’t know them. Trends Biochem. Sci. 27, 564–572. CrossRefGoogle Scholar
Turrens, J.F., 2003, Mitochondrial formation of reactive oxygen species. J. Physiol. 552, 335–344. CrossRefGoogle Scholar
Xu, M., Wang, Y., Ayub, A., Ashraf, M., 2001, Mitochondrial K-ATP channel activation reduces anoxic injury by restoring mitochondrial membrane potential. Am. J. Physiol. Heart Circ. Physiol. 281, H1295–H1303. Google Scholar