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Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass, Dicentrarchus labrax

Published online by Cambridge University Press:  04 October 2010

Nolwenn Quéré
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France
Bruno Guinand*
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France Station Méditerranéenne de l’Environnement Littoral, 1 quai de la Daurade, 34200 Sète, France
Heiner Kuhl
Affiliation:
Max-Planck-Institute Molecular Genetics , Ihnestrasse 63-73, 14195 Berlin-Dahlem, Germany
Richard Reinhardt
Affiliation:
Max-Planck-Institute Molecular Genetics , Ihnestrasse 63-73, 14195 Berlin-Dahlem, Germany
François Bonhomme
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France Station Méditerranéenne de l’Environnement Littoral, 1 quai de la Daurade, 34200 Sète, France
Erick Desmarais
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France
*
a Corresponding author: bruno.guinand@univ-montp2.fr
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Abstract

The completion of genomic sequences of physiologically important genes frequently reveals non-coding genetic elements such as tandem repeats (micro- and minisatellites) that are often more polymorphic than nearby coding sequences. We obtained the complete genomic sequences of three hormone genes in sea bass Dicentrarchus labrax: growth hormone (dlGH), somatolactin (dlSL) and insulin-like growth factor-1 (dlIGF-1), including 5′- and 3′-untranslated regions. Mini- and microsatellites were discovered in both flanking and intron regions. Some were partially conserved across Perciformes. To assess the usefulness and relevance of these gene-associated markers for understanding population structure, an investigation was made on genetic diversity and differentiation at four of them in (i) five wild populations from the North Sea, the Bay of Biscay and the Western Mediterranean, and (ii) two samples of hatchery-bred individuals from a freshwater-acclimation experiment. Gene and allelic diversities were lower in cultured individuals than in wild ones. Significant genetic differentiation was demonstrated between Bay of Biscay + North Sea and Mediterranean populations (F st > 0.06, p < 0.001), primarily due to dlGH-associated markers. Significant genetic differentiation was also detected among the Atlantic and North Sea samples, but restricted to the locus associated with dlSL. Significant genetic differentiation was also found among experimental individuals before and after a salinity challenge (F st ≈ 0.05, p < 0.001), but was due to dlSL and dlIGF-1 loci. Gene-associated markers proved to be more efficient than formerly used anonymous microsatellite markers in providing a clear picture of genetic differentiation.

Type
Research Article
Copyright
© EDP Sciences, IFREMER, IRD 2010

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Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass, Dicentrarchus labrax
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Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass, Dicentrarchus labrax
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