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Stable isotope ratios of blood components and muscle to trace dietary changes in lambs

Published online by Cambridge University Press:  19 April 2013

L. Biondi ,
M. G. D'Urso ,
V. Vasta ,
G. Luciano ,
M. Scerra ,
A. Priolo ,
L. Ziller ,
L. Bontempo ,
P. Caparra  and
F. Camin
L. Biondi*
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
M. G. D'Urso
Affiliation:
Dottorato di Ricerca in Scienze delle Produzioni Animali, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
V. Vasta
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
G. Luciano
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
M. Scerra
Affiliation:
Department STAFA, Division of Animal Production, University of Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy
A. Priolo
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
L. Ziller
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
L. Bontempo
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
P. Caparra
Affiliation:
Department STAFA, Division of Animal Production, University of Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy
F. Camin
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
*
†E-mail: lubiondi@unict.it
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Abstract

Multielemental stable isotope ratio (SIR) analysis was used in lamb plasma, erythrocytes and muscle to detect the switch from a pasture- to a concentrate-based diet, with the aim of verifying the possibility to trace the change of feeding in animal tissues. During 89 days of experimental feeding, lambs were subjected to four dietary treatments: pasture (P), pasture followed by concentrate in the stall for either 14 days (P-S14) or 37 days (P-S37) or concentrate in the stall (S). Pasture and concentrate diets comprised C3 plants only and had different values of 13C/12C, 18O/16O, 2H/1H and 34S/32S ratios. Muscle 13C/12C and 34S/32S and plasma 13C/12C and 18O/16O ratios in P, P-S14 and P-S37 lambs were significantly different. A multivariate analytical approach revealed that 13C/12C and 18O/16O ratios in plasma were the most powerful variables for the discrimination among the dietary treatments.

Keywords

IRMSlamb musclelamb plasmatraceabilitydiet
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 9 , September 2013 , pp. 1559 - 1566
Copyright
Copyright © The Animal Consortium 2013 

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