Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-17T23:35:50.580Z Has data issue: false hasContentIssue false
  • English
  • Français

Screening of plants from diversified natural grasslands for their potential to combine high digestibility, and low methane and ammonia production

Published online by Cambridge University Press:  21 July 2014

D. Macheboeuf ,
L. Coudert ,
R. Bergeault ,
G. Lalière  and
V. Niderkorn
D. Macheboeuf
Affiliation:
INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France
L. Coudert
Affiliation:
INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France
R. Bergeault
Affiliation:
INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France
G. Lalière
Affiliation:
Ethnobotanique Guy Lalière, 16 rue des Plats, F-63000 Clermont-Ferrand, France
V. Niderkorn*
Affiliation:
INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France
*
† E-mail: vincent.niderkorn@clermont.inra.fr
Get access

Abstract

A total of 156 plant species from 35 botanical families collected from diversified grasslands in the French Massif Central were screened in vitro for their potential to combine high nutritive value for ruminants and a reduced impact on the environment. The vegetative part of plants were analyzed for their chemical composition and incubated in a batch system containing buffered rumen fluid at 39°C for 24 h. The gas production and composition were recorded, and the fermentation end-product concentrations in the incubation medium and the in vitro true organic matter digestibility (IVTOMD) were determined. The results were expressed relative to perennial ryegrass (PRG) values used as a reference. We observed that no relationship between methane (CH4) and volatile fatty acids (VFA) was evidenced for 12 plants, the fermentation of these plants producing significantly less CH4 for a similar level of VFA production. In all, 13 plants showed 50% less CH4 production per unit of organic matter truly digested (OMTD) than PRG. Among these plants, two reduced CH4 by more than 80% and four species had an IVTOMD higher than 80%. The underlying modes of action seem to be different among plants: some result in an accumulation of H2 in the fermentation gas, but others do not. In terms of nitrogen (N) use efficiency, the fermentation of 37 plants halved the ratio between ammonia (N–NH3) and plant N content compared with PRG, of which six showed a complete absence of N–NH3 in the medium. Among these plants, four maintained the IVTDMO at values not significantly different from PRG (P>0.05). Considering the multi-criteria selection, 16 plants showed simultaneously a reduction of more than 80% in N–NH3 production and 30% in CH4 emission per unit of OMTD compared with PRG, including three with an IVTOMD higher than 80%. Overall, the botanical families that reduced simultaneously CH4 and N–NH3 most efficiently were the Rosaceae, Onagraceae, Polygonaceae and Dipsacaceae. The Onagraceae also gave high values for IVTOMD.

Keywords

agroecologygrassland plantsrumen fermentationmethanenitrogen metabolism
Type
Research Article
Information
animal , Volume 8 , Issue 11 , November 2014 , pp. 1797 - 1806
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Association of Official Analytical Chemists 1995. Official methods of analysis, method 990.03, 16th edition. AOAC, Washington, DC.Google Scholar
Battinelli, L, Tita, B, Evandri, MG and Mazzanti, G 2001. Antimicrobial activity of Epilobium spp. extracts. Farmaco (Società chimica italiana: 1989) 56, 345–348.Google Scholar
Bodas, R, Giráldez, F, Rodríguez, A, Wallace, R, González, J and López, S 2011. Effects of the inclusion of Knautia arvensis in the concentrate for fattening lambs on feed intake, digestibility and growth performances. Options Méditerranéennes: Série A 99, 219–222.Google Scholar
Bodas, R, López, S, Fernández, M, García-González, R, Rodríguez, A, Wallace, R and González, J 2008. In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants. Animal Feed Science and Technology 145, 245–258.Google Scholar
Broudiscou, LP and Lassalas, B 2000. Effects of Lavandula officinalis and Equisetum arvense dry extracts and isoquercitrin on the fermentation of diets varying in forage contents by rumen microorganisms in batch culture. Reproduction, Nutrition, Development 40, 431–440.Google Scholar
Calsamiglia, S, Ferret, A, Reynolds, C, Kristensen, N and Van Vuuren, A 2010. Strategies for optimizing nitrogen use by ruminants. Animal 4, 1184–1196.CrossRefGoogle ScholarPubMed
Chaney, AL and Marbach, EP 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry 8, 130–132.CrossRefGoogle ScholarPubMed
Chaucheyras, F, Fonty, G, Bertin, G and Gouet, P 1995. In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an Archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae . Applied and Environmental Microbiology 61, 3466–3467.Google Scholar
Clergue, B, Amiaud, B, Pervanchon, F, Lasserre-Joulin, F and Plantureux, S 2005. Biodiversity: function and assessment in agricultural areas. A review. Agronomy for Sustainable Development 25, 1–15.Google Scholar
Demeyer, DI 1991. Quantitative aspects of microbial metabolism in the rumen and hindgut. In Rumen microbial metabolism and ruminant digestion (ed. JP Jouany), pp. 217–237. INRA Editions, Paris, France.Google Scholar
Dumont, B, Fortun-Lamothe, L, Jouven, M, Thomas, M and Tichit, M 2013. Prospects from agroecology and industrial ecology for animal production in the 21st century. Animal 7, 1028–1043.Google Scholar
Dumont, B, Garel, J, Ginane, C, Decuq, F, Farruggia, A, Pradel, P, Rigolot, C and Petit, M 2007. Effect of cattle grazing a species-rich mountain pasture under different stocking rates on the dynamics of diet selection and sward structure. Animal 1, 1042–1052.Google Scholar
Durmic, Z, Hutton, P, Revell, D, Emms, J, Hughes, S and Vercoe, P 2010. In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants. Animal Feed Science and Technology 160, 98–109.Google Scholar
Hsu, C 2006. Antioxidant activity of extract from Polygonum aviculare L. Biological Research 39, 281–288.Google Scholar
Kim, HJ, Woo, E-R and Park, H 1994. A novel lignan and flavonoids from Polygonum aviculare . Journal of Natural Products 57, 581–586.CrossRefGoogle Scholar
Macheboeuf, D, Morgavi, DP, Papon, Y, Mousset, JL and Arturo-Schaan, M 2008. Dose-response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Animal Feed Science and Technology 145, 335–350.CrossRefGoogle Scholar
Makkar, HPS, Francis, G and Becker, K 2007. Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. Animal 1, 1371–1391.Google Scholar
McAllister, T and Newbold, CJ 2008. Redirecting rumen fermentation to reduce methanogenesis. Animal Production Science 48, 7–13.CrossRefGoogle Scholar
McArt, SH, Spalinger, DE, Kennish, JM and Collins, WB 2006. A modified method for determining tannin–protein precipitation capacity using accelerated solvent extraction (ASE) and microplate gel filtration. Journal of Chemical Ecology 32, 1367–1377.CrossRefGoogle ScholarPubMed
Morgavi, D, Forano, E, Martin, C and Newbold, CJ 2010. Microbial ecosystem and methanogenesis in ruminants. Animal 4, 1024–1036.Google Scholar
Morvan, B, Dore, J, Rieu-Lesme, F, Foucat, L, Fonty, G and Gouet, P 1994. Establishment of hydrogen-utilizing bacteria in the rumen of the newborn lamb. FEMS Microbiology Letters 117, 249–256.CrossRefGoogle ScholarPubMed
Niderkorn, V, Baumont, R, Le Morvan, A and Macheboeuf, D 2011. Occurrence of associative effects between grasses and legumes in binary mixtures on in vitro rumen fermentation characteristics. Journal of Animal Science 89, 1138–1145.Google Scholar
Pacha, MJ and Petit, S 2008. The effect of landscape structure and habitat quality on the occurrence of Geranium sylvaticum in fragmented hay meadows. Agriculture, Ecosystems & Environment 123, 81–87.Google Scholar
Patra, AK 2010. Meta‐analyses of effects of phytochemicals on digestibility and rumen fermentation characteristics associated with methanogenesis. Journal of the Science of Food and Agriculture 90, 2700–2708.CrossRefGoogle ScholarPubMed
Patra, AK and Saxena, J 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry 71, 1198–1222.Google Scholar
Peng, ZF, Strack, D, Baumert, A, Subramaniam, R, Goh, NK, Chia, TF, Ngin Tan, S and Chia, LS 2003. Antioxidant flavonoids from leaves of Polygonum hydropiper L. Phytochemistry 62, 219–228.Google Scholar
Rockström, J, Steffen, W, Noone, K, Persson, Å, Chapin, FS, Lambin, EF, Lenton, TM, Scheffer, M, Folke, C and Schellnhuber, HJ 2009. A safe operating space for humanity. Nature 461, 472–475.Google Scholar
Sandu, R, Tarţău, L, Miron, A, Zagnat, M, Ghiciuc, C and Lupuşoru, C 2012. Experimental researches on acute toxicity of a Bidens tripartita extract in mice – Preliminary investigation. Medical-Surgical Journal of the Society of Physicians and Naturalists, Iaşi 116, 1230–1234.Google Scholar
Selje, N, Hoffmann, EM, Muetzel, S, Ningrat, R, Wallace, RJ and Becker, K 2007. Results of a screening programme to identify plants or plant extracts that inhibit ruminal protein degradation. British Journal of Nutrition 98, 45–53.Google Scholar
Sesterhenn, K, Distl, M and Wink, M 2007. Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L. Plant Cell Reports 26, 365–371.Google Scholar
Steinfeld, H and Wassenaar, T 2007. The role of livestock production in carbon and nitrogen cycles. Annual Review of Environment and Resources 32, 271–294.Google Scholar
Theodoridou, K, Aufrère, J, Andueza, D, Pourrat, J, Le Morvan, A, Stringano, E, Mueller-Harvey, I and Baumont, R 2010. Effects of condensed tannins in fresh sainfoin (Onobrychis viciifolia) on in vivo and in situ digestion in sheep. Animal Feed Science and Technology 160, 23–38.Google Scholar
Theodorou, MK, Williams, BA, Dhanoa, MS, McAllan, AB and France, J 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48, 185–197.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Van Zijderveld, S, Gerrits, W, Apajalahti, J, Newbold, J, Dijkstra, J, Leng, R and Perdok, H 2010. Nitrate and sulfate: effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep. Journal of Dairy Science 93, 5856–5866.Google Scholar
Wojdyło, A, Oszmiański, J and Czemerys, R 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry 105, 940–949.Google Scholar