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A note on heat shock protein 70 expression in goats subjected to road transportation under hot, humid tropical conditions

Published online by Cambridge University Press:  26 February 2010

I. Zulkifli ,
B. Norbaiyah ,
Y. W. Cheah ,
A. F. Soleimani ,
A. Q. Sazili ,
Y. M. Goh  and
M. A. Rajion
I. Zulkifli*
Affiliation:
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
B. Norbaiyah
Affiliation:
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Y. W. Cheah
Affiliation:
Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
A. F. Soleimani
Affiliation:
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
A. Q. Sazili
Affiliation:
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Y. M. Goh
Affiliation:
Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
M. A. Rajion
Affiliation:
Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
*
†E-mail: zulkifli@agri.upm.edu.my
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Abstract

The influence of two different stocking densities (0.20 m2/animal and 0.40 m2/animal) in transit under the hot, humid tropical conditions on heat shock protein (hsp) 70 induction was investigated in 60 Boer does. The animals were road transported for 3 h and the control group was kept under normal conditions in the farm. Irrespective of stocking density, transportation significantly increased hsp 70 densities (P < 0.05) in the kidneys. The hsp 70 response in the kidneys was more profound compared with those of heart tissues. Higher stocking density was more stressful to the goats based on hsp 70 expression. These results suggest that, irrespective of stocking density, transportation under hot, humid tropical conditions evoked hsp 70 reactions.

Keywords

transportationheat shock proteingoats
Type
Full Paper
Information
animal , Volume 4 , Issue 6 , June 2010 , pp. 973 - 976
Copyright
Copyright © The Animal Consortium 2010

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References

Al-Aqil, A, Zulkifli, I 2009. Changes in heat shock protein 70 expression and blood parameters in transported broiler chickens as affected by housing and early age feed restriction. Poultry Science 88, 1358–1364.CrossRefGoogle ScholarPubMed
Animal Welfare Guidelines-Road Transport of Livestock within Tasmania 2008. Retrieved January 8, 2010, from http://www.dpiw.tas.gov.au/inter.nsf/Attachments/LBUN-7T39TF/$FILE/Animal%20Welfare%20Guidelines-Road%20Transport%20of%20Livestock%20Within%20Tasmania.pdfGoogle Scholar
Barbe, MF, Tytell, M, Gower, DJ, Welch, WJ 1988. Hyperthermia protects against light damage in the rat retina. Science 241, 1817–1820.CrossRefGoogle ScholarPubMed
Blake, MJR, Udelsman, R, Feulner, GJ, Norton, DD, Holbrook, NJ 1991. Stress-induced HSP70 expression in adrenal cortex: a glucocorticoid-sensitive, age-dependent response. Proceedings of the National Academy of Sciences of the United States of America 88, 9873–9877.CrossRefGoogle Scholar
Broom, DM 2000. Welfare assessment and welfare problem areas during handling and transport. In Livestock handling and transport (ed. TG Grandin), pp. 43–62. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
Chirico, WJ, Waters, MG, Blobel, G 1988. 70 K heat shock related proteins stimulate protein translocation into microsomes. Nature 332, 805–810.CrossRefGoogle Scholar
Cullen, KE, Sarge, KD 1997. Characterization of hypothermia-induced cellular stress response in mouse tissues. The Journal of Biological Chemistry 272, 1742–1746.CrossRefGoogle ScholarPubMed
Etches, RJ, John, TM, Verrinder Gibbins, AM 1995. Behavioural, physiological, neuroendocrine and molecular responses to heat stress. In Poultry production in hot climates (ed. DJ Daghir), pp. 31–65. CAB International, Wallingford, UK.Google Scholar
Knowles, TG 1998. A review of road transport of sheep. Veterinary Record 143, 212–219.CrossRefGoogle Scholar
Koh, TJ 2002. Do small heat shock proteins protect skeletal muscle from injury? Exercise and Sport Sciences. Reviews 30, 117–121.Google Scholar
Laemmli, UK 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Latchman, DS 2001. Heat shock proteins and cardiac protection. Cardiovascular Research 51, 637–646.CrossRefGoogle ScholarPubMed
Matz, JM, Blake, MJ, Tatelman, HM, Lavoi, KP, Holbrook, NJ 1995. Characterization and regulation of cold-induced heat shock protein expression in mouse brown adipose tissue. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 269, 38–47.Google ScholarPubMed
SAS Institute 1991. SAS/STAT User’s Guide, release 6.03. SAS Institute, Cary, NC, USA.Google Scholar
Tarrant, PV, Kenny, FJ, Harrington, D, Murphy, M 1992. Long distance transportation of steers to slaughter: effect of stocking density on physiology, behaviour and carcass quality. Livestock Production Science 30, 223–238.CrossRefGoogle Scholar
Towbin, H, Stachelin, T, Gordon, J 1979. Electrophoretic transfer of proteins frompolyacrilamide gels into nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76, 4350–4354.CrossRefGoogle ScholarPubMed
Turman, MA, Rosenfeld, SL 1999. Heat shock protein 70 over expression protects LLC-PKI tubular cells from heat shock but not hypoxia. Kidney International 55, 189–197.CrossRefGoogle Scholar
Yu, Hong, Bao, En-dong, Zhao, Ru-qian, , Qiong-xia 2007. Effect of transportation stress on heat shock protein 70 concentration and mRNAexpression in heart and kidney tissues and serum enzyme activities and hormone concentrations of pigs. American Journal of Veterinary Research 68, 1145–1150.CrossRefGoogle Scholar
Zulkifli, I, Al-Aqil, A, Omar, AR, Sazili, AQ, Rajion, MA 2008. Housing system affects stress and fear reactions, and meat quality of broiler chickens subjected to road transportation. In Proceedings of the XXIII World’s Poultry Congress 2008, p. 380. World’s Poultry Science Association, Odijk, The Netherlands.Google Scholar
Zulkifli, I, Al-Aqil, A, Omar, AR, Sazili, AQ, Rajion, MA 2009. Crating and heat stress influences blood parameters and heat shock protein 70 expression in broiler chickens showing short or long tonic immobility reactions. Poultry Science 88, 471–476.CrossRefGoogle ScholarPubMed
Zulkifli, I, Che Norma, MT, Chong, CH, Loh, TC 2001. The effects of crating and road transportation on stress and fear responses of broiler chickens treated with ascorbic acid in the hot, humide tropics. Archiv für Geflügelkunde 65, 33–37.Google Scholar
Zulkifli, I, Liew, PK, Israf, DA, Omar, AR, Hair-Bejo, M 2003. Effects of early age feed restriction and thermal conditioning on heterophil/lymphocyte ratio, heat shock protein 70 and body temperature of male broiler chickens subjected to acute heat stress. Journal of Thermal Biology 28, 217–222.CrossRefGoogle Scholar