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Naturally and stimulated levels of reactive oxygen species in cooled stallion semen destined for artificial insemination

Published online by Cambridge University Press:  11 June 2014

A. Johannisson ,
A. Lundgren ,
P. Humblot  and
J. M. Morrell
A. Johannisson*
Affiliation:
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Box 7011, Uppsala, Sweden
A. Lundgren
Affiliation:
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Box 7054, Uppsala, Sweden
P. Humblot
Affiliation:
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Box 7054, Uppsala, Sweden
J. M. Morrell
Affiliation:
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Box 7054, Uppsala, Sweden
*
†E-mail: Anders.Johannisson@slu.se
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Abstract

The decrease in foaling rates after artificial insemination with cooled semen warrants the search for new predictors of fertility. The objectives were to investigate levels of naturally occurring reactive oxygen species (ROS) in cooled, stored stallion semen doses for artificial insemination (AI), and their relationship with parameters of semen quality and with pregnancy rate. Semen was collected from warmblood stallions (n=15) and used to prepare commercial semen doses for AI. Sperm quality was evaluated after cooled transport to the laboratory overnight. The results were correlated with observed foaling and pregnancy rates. Hydroethidine and dichlorodihydrofluorescein diacetate were used as indicators for the ROS superoxide and hydrogen peroxide, respectively. Sperm morphology, motility, plasma membrane integrity and chromatin integrity were also evaluated. These variables were correlated with each other and with pregnancy rates. We found a high inter-individual variation in the ROS levels between stallions. The proportion of live, hydrogen peroxide-negative spermatozoa was correlated with progressive motility, whereas live hydrogen peroxide-negative spermatozoa and chromatin damage were negatively correlated, indicating that low levels of hydrogen peroxide were correlated with good chromatin integrity. The percentage of dead hydrogen peroxide-positive sperm was negatively related to the foaling rate. The negative relationships were stronger when combining results from both assays for ROS. These results for stored semen samples indicate that high individual variation exists for superoxide and hydrogen peroxide measurements, and that ROS status can influence sperm quality. Thus, ROS may be some of the factors influencing fertility. Moreover, combinations of ROS variables improved the correlation with fertility, indicating the usefulness of including these variables in a future model for prediction of the fertility of a semen sample.

Keywords

stallionartificial inseminationcooled semenreactive oxygen speciesfertility
Type
Research Article
Information
animal , Volume 8 , Issue 10 , October 2014 , pp. 1706 - 1714
Copyright
© The Animal Consortium 2014 

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