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Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

Published online by Cambridge University Press:  01 January 2008

M. Mota ,
J. Balcells ,
N. H. Ozdemir Baber ,
S. Bölüktepe  and
A. Belenguer
M. Mota
Affiliation:
Departamento Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, C/Miguel Servet 177, Zaragoza, Spain
J. Balcells*
Affiliation:
Departamento Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, C/Miguel Servet 177, Zaragoza, Spain
N. H. Ozdemir Baber
Affiliation:
Turkish Atomic Energy Authorytỳ Saraykov Nuclear Reserach and Training Center, Ist. Yolu. 30 Km Saray Kazan/Ankara, Turkey
S. Bölüktepe
Affiliation:
Gida Kontrol ve Merkez Araştirma, Enstitüsü Müdürlügü, 3 16036 Hüririyet/Bursa, Turkey
A. Belenguer
Affiliation:
Departamento Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, C/Miguel Servet 177, Zaragoza, Spain
*
E-mail: Balcells@unizar.es
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Abstract

To determine the endogenous contribution of purine derivatives (PD) to renal excretion and the urinary recovery of duodenal purine bases (PB), five dairy Granadina goats (initial weight ± s.e.: 38.6 ± 2.78 kg) were each fitted with a duodenal infusion catheter. Animals were offered ad libitum a mixed diet (75 : 25; alfalfa hay : concentrate), which was supplied in equal portions every 3 h. To label microbial PB, (15NH4)2SO4 was added to the concentrate. The lower enrichment of urinary PD (15N-allantoin) compared with duodenal PB enrichment confirmed the presence of an endogenous PD fraction (268.5 ± 21.98 μmol/kg weight0.75 or 0.386 of the total PD excretion). The recovery of PD in urine and milk increased linearly in response to increasing amounts of duodenally infused RNA (starting on day 21 after parturition). On average, 0.74 of infused PB from RNA was recovered in urine. Milk PD constituted a minor (<0.01) fraction of the total PD excretion and this fraction decreased as the amount of infused PB increased. Our findings indicate that lactation in goats did not affect the urinary recovery of duodenal PB but increased the endogenous contribution to urinary excretion of PD.

Keywords

dairy goatsmicrobial synthesismilkpurine derivativesurinary excretion
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 44 - 51
Copyright
Copyright © The Animal Consortium 2008

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