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Microsatellite mapping of quantitative trait loci affecting meat quality, stress hormones and production traits in Duroc × Large White F2 pigs

Published online by Cambridge University Press:  06 September 2010

M. P. Sanchez ,
N. Iannuccelli ,
B. Basso ,
A. Foury ,
Y. Billon ,
G. Gandemer ,
H. Gilbert ,
P. Mormède ,
J. P. Bidanel  and
C. Larzul
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M. P. Sanchez*
Affiliation:
INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
N. Iannuccelli
Affiliation:
INRA, UR444 Laboratoire de génétique cellulaire, F-31320 Castanet-Tolosan, France
B. Basso
Affiliation:
INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
A. Foury
Affiliation:
INRA, UMR1286; CNRS, UMR5226, Laboratoire PsyNuGen, F-33076 Bordeaux, France
Y. Billon
Affiliation:
INRA, UE967 Génétique expérimentale en productions animales, F-17700 Surgères, France
G. Gandemer
Affiliation:
INRA, UAR2 Services déconcentrés d’appui à la recherche – Poitou-Charentes, F-17700 Surgères, France
H. Gilbert
Affiliation:
INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
P. Mormède
Affiliation:
INRA, UMR1286; CNRS, UMR5226, Laboratoire PsyNuGen, F-33076 Bordeaux, France
J. P. Bidanel
Affiliation:
INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
C. Larzul
Affiliation:
INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
J. Riquet
Affiliation:
INRA, UR444 Laboratoire de génétique cellulaire, F-31320 Castanet-Tolosan, France
D. Milan
Affiliation:
INRA, UR444 Laboratoire de génétique cellulaire, F-31320 Castanet-Tolosan, France
P. Le Roy
Affiliation:
INRA-Agrocampus Rennes, UMR598 Génétique animale, F-35042 Rennes, France
*
†E-mail: marie-pierre.sanchez@jouy.inra.fr
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Abstract

An F2 cross between Duroc and Large White pigs was carried out in order to detect quantitative trait loci (QTL) for 11 meat quality traits (L*, a* and b* Minolta coordinates and water-holding capacity (WHC) of two ham muscles, ultimate pH of two ham and one loin muscles), 13 production traits (birth weight, average daily gain during post-weaning and fattening periods, carcass fat depths at three locations, estimated lean meat content, carcass length and weights of five carcass cuts) and three stress hormone-level traits (cortisol, adrenaline and noradrenaline). Animals from the three generations of the experimental design (including 456 F2 pigs) were genotyped for 91 microsatellite markers covering all the autosomes. A total of 56 QTL were detected: 49 reached the chromosome-wide level (suggestive QTL with a maximal probability of 0.05) and seven were significant at the genome-wide level (with a probability varying from 6 × 10−4 to 3 × 10−3). Twenty suggestive QTL were identified for ultimate pH, colour measurements and WHC on chromosome (SSC) 5, 6, 7, 8, 9, 11, 13, 14, 15 and 17. For production traits, 33 QTL were detected on all autosomes except SSC6, 8 and 9. Seven of these QTL, located on SSC2, 3, 10, 13, 16 and 17, exceeded the genome-wide significance threshold. Finally, three QTL were identified for levels of stress hormones: a QTL for cortisol level on SSC7 in the cortisol-binding globulin gene region, a QTL for adrenaline level on SSC10 and a QTL for noradrenaline level on SSC13. Among all the detected QTL, seven are described for the first time: a QTL for ultimate pH measurement on SSC5, two QTL affecting birth weight on SSC2 and 10, two QTL for growth rate on SSC15 (during fattening) and 17 (during post-weaning) and two QTL affecting the adrenaline and noradrenaline levels. For each QTL, only one to five of the six F1 sires were found to be heterozygous. It means that all QTL are segregating in at least one of the founder populations used in this study. These results suggest that both meat quality and production traits can be improved in purebred Duroc and Large White pigs through marker-assisted selection. It is of particular interest for meat quality traits, which are difficult to include in classical selection programmes.

Keywords

pigQTLmeat qualityproductionstress hormones
Type
Full Paper
Information
animal , Volume 5 , Issue 2 , February 2011 , pp. 167 - 174
Copyright
Copyright © The Animal Consortium 2010

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