Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-20T04:51:23.232Z Has data issue: false hasContentIssue false
  • English
  • Français

Metabolic and stress responses in dairy cows fed a concentrate-rich diet and submitted to intramammary lipopolysaccharide challenge

Published online by Cambridge University Press:  12 September 2017

S. Aditya ,
E. Humer ,
P. Pourazad ,
R. Khiaosa-ard  and
Q. Zebeli
S. Aditya
Affiliation:
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria Directorate General of Human Resource for Science, Technology, and Higher Education-Indonesia, 10270 Jakarta, Indonesia
E. Humer
Affiliation:
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria
P. Pourazad
Affiliation:
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria
R. Khiaosa-ard
Affiliation:
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria
Q. Zebeli*
Affiliation:
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria
*
†E-mail: Qendrim.Zebeli@vetmeduni.ac.at
Get access

Abstract

Feeding dairy cows diets rich in grain often leads to subacute rumen acidosis (SARA), which might affect their responsiveness to immunogenic stimuli such as exogenous lipopolysaccharide (LPS), and can lead to metabolic alterations. The main objective of this study was to investigate if SARA affects the stress and metabolic health responses resulting from an intramammary LPS challenge. Before the intramammary LPS challenge, the SARA cows showed higher blood glucose and a tendency for higher lactate and aspartate aminotransferase as well as a trend toward lower β-hydroxybutyrate (BHBA) and γ-glutamyltransferase compared with control cows. After the LPS challenge, the serum cortisol concentration markedly increased and the calcium concentration decreased both in SARA and control cows. In SARA-LPS cows, however, the lactate concentration increased due to the LPS infusion, whereas it remained unchanged in the control cows. A lower serum BHBA concentration was found in SARA-LPS compared with control-LPS cows. Higher non-esterified fatty acid concentrations were found in control-LPS cows shortly before the LPS challenge compared with SARA cows, challenged or not with LPS, whereas it did not differ from SARA-LPS cows thereafter. In conclusion, the results suggest that intramammary LPS challenge induced stress and lowered calcium concentration in all dairy cows, whereby this challenge showed lower BHBA and higher lactate levels in cows with SARA conditions.

Keywords

dairy cowlipopolysaccharidestressmetabolic responsesubacute rumen acidosis
Type
Research Article
Information
animal , Volume 12 , Issue 4 , April 2018 , pp. 741 - 749
Copyright
© The Animal Consortium 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aditya, S, Humer, E, Pourazad, P, Khiaosa-ard, R, Huber, J and Zebeli, Q 2017. Intramammary infusion of Escherichia coli lipopolysaccharide negatively affects feed intake, chewing behavior, milk and clinical parameters, but some effects are stronger in cows experiencing subacute rumen acidosis. Journal of Dairy Science 100, 1363–1377.CrossRefGoogle Scholar
Ametaj, BN, Emmanuel, DGV, Zebeli, Q and Dunn, SM 2009. Feeding high proportions of barley grain in a total mixed ration perturbs diurnal patterns of plasma metabolites in lactating dairy cows. Journal of Dairy Science 92, 1084–1091.CrossRefGoogle Scholar
Ametaj, BN, Zebeli, Q and Iqbal, S 2010. Nutrition, microbiota, and endotoxin-related diseases in dairy cows. Revista Brasileira de Zootecnia 39, 433–444.CrossRefGoogle Scholar
Andersen, PH 2003. Bovine endotoxicosis – some aspects of relevance to production diseases. A review. Acta Veterinaria Scandinavica 98, 141–155.Google ScholarPubMed
Aschenbach, JR, Kristensen, NB, Donkin, SS, Hammon, HM and Penner, GB 2010. Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough. IUBMB Life 62, 869–877.CrossRefGoogle ScholarPubMed
Beishuizen, A and Thijs, LG 2003. Endotoxin and the hypothalamo-pituitary adrenal (HPA) axis. Journal of Endotoxin Research 9, 3–24.Google ScholarPubMed
Bines, JA and Davey, AW 1978. Metabolic changes associated with intake by cows of complete diets containing straw and concentrates in different proportions. British Journal of Nutrition 39, 567–578.CrossRefGoogle ScholarPubMed
Bobe, G, Young, JW and Beitz, DC 2004. Invited review: pathology, etiology, prevention, and treatment of fatty liver in dairy cows. Journal of Dairy Science 87, 3105–3104.CrossRefGoogle ScholarPubMed
Caton, PW, Nayuni, NK, Murch, O and Corder, R 2009. Endotoxin induced hyperlactatemia is linked to decreased mitochondrial phosphoenol pyruvatecarboxykinase. Life Science 84, 738–744.CrossRefGoogle Scholar
Cozzi, G, Ravarotto, L, Gottardo, F, Stefani, AL, Contiero, B, Moro, L, Brscic, M and Dalvit, P 2011. Short communication: reference values for blood parameters in Holstein dairy cows: effects of parity, stage of lactation, and season of production. Journal of Dairy Science 94, 3895–3901.CrossRefGoogle ScholarPubMed
De Koster, JD and Opsomer, G 2013. Insulin resistance in dairy cows. Veterinary Clinics of North America: Food Animal Practice 29, 299–322.Google ScholarPubMed
Eckel, EF and Ametaj, BN 2016. Invited review: role of bacterial endotoxins in the etiopathogenesis of periparturient diseases of transition dairy cows. Journal of Dairy Science 99, 5967–5990.CrossRefGoogle ScholarPubMed
Eggesbø, JB, Lyberg, T, Aspelin, T, Hjermann, I and Kierulf, P 1996. Different binding of 125I-LPS to plasma proteins from persons with high or low HDL. Scandinavian Journal of Clinical and Laboratory Investigation 56, 533–543.CrossRefGoogle ScholarPubMed
German Society of Nutrition Physiology (GfE) 2001. Recommendations for the supply of energy and nutrients to dairy cows an heifers. Committee for requirement standards of the society of nutrition physiology (in German). DLG Verlag, Frankfurt am Main, Germany.Google Scholar
Kaneko, JJ, Harvey, JW and Bruss, ML 2008. Clinical biochemistry of domestic animals, 6th edition. Elsivier’s Inc, Oxford, UK.Google Scholar
Kaur, G, Tirkey, N, Bharrhan, S, Chanana, V, Rishi, P and Chopra, K 2006. Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents. Clinical and Experimental Immunology 145, 313–321.CrossRefGoogle ScholarPubMed
Khafipour, E, Li, S, Plaizier, JC and Krause, DO 2009. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Applied and Environmental Microbiology 75, 7115–7124.CrossRefGoogle ScholarPubMed
Kobayashi, K, Oyama, S, Numata, A, Rahman, MdM and Kumura, HK 2013. Lipopolysaccharide disrupts the milk-blood barrier by modulating claudins in mammary alveolar tight junctions. PLoS One 8, e62187.CrossRefGoogle ScholarPubMed
Kushibiki, S, Hodate, K, Shingu, H, Hayashi, T, Touno, E, Shinoda, M and Yokomizo, Y 2002. Alterations in lipid metabolism induced by recombinant bovine tumor necrosis factor-alpha administration to dairy heifers. Journal of Animal Science 80, 2151–2157.Google ScholarPubMed
Li, S, Gozho, G, Gakhar, N, Khafipour, E, Krause, D and Plaizier, J 2012. Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows. Canadian Journal of Animal Science 92, 353–364.CrossRefGoogle Scholar
Lüttgenau, J, Wellnitz, O, Kradolfer, D, Kalaitzakis, E, Ulbrich, SE, Bruckmaier, RM and Bollwein, H 2016. Intramammary lipopolysaccharide infusion alters gene expression but does not induce lysis of the bovine corpus luteum. Journal of Dairy Science 99, 1–14.Google Scholar
McDonald, P, Edwards, RA, Greenhalgh, JFD, Morgan, CA, Sinclair, LA and Wilkinson, RG 2011. Animal nutrition, 7th edition. Pearson Education Limited, Essex, UK.Google Scholar
Morris, M and Li, L 2012. Molecular mechanisms and pathological consequences of endotoxin tolerance and priming. Archivum Immunologiae et Therapiae Experimentalis 60, 13–18.CrossRefGoogle ScholarPubMed
Moyes, KM, Larsen, T, Sǿrensen, P and Ingvartsen, KL 2014. Changes in various metabolic parameters in blood and milk during experimental Escherichia coli mastitis for primiparous Holstein dairy cows during early lactation. Journal of Animal Science and Biotechnology 5, 47.CrossRefGoogle ScholarPubMed
Neubauer, V, Humer, E, Kröger, I, Braid, T, Wagner, M and Zebeli, Q 2017. Short communication: differences between pH of indwelling sensors and the pH of fluid and solid phase in the rumen of dairy cows fed varying concentrate levels. Journal of Animal Physiology and Animal Nutrition. https://doi.org/10.1111/jpn.12675.CrossRefGoogle Scholar
Ospina, PA, Nydam, DV, Stokol, T and Overton, TR 2010. Evaluation of nonesterified fatty acids and beta-hydroxybutyrate in transition dairy cattle in the northeastern United States: critical thresholds for prediction of clinical diseases. Journal of Dairy Science 93, 546–554.CrossRefGoogle ScholarPubMed
Plaizier, JC, Khafipour, E, Li, S, Gozho, GN and Krause, DO 2012. Subacute ruminal acidosis (SARA), endotoxins, and health consequences. Animal Feed Science and Technology 172, 9–21.CrossRefGoogle Scholar
Qumar, M, Khiaosa-Ard, R, Pourazad, P, Wetzels, S, Klevenhusen, F, Kandler, W, Aschenbach, J and Zebeli, Q 2016. Evidence of in vivo absorption of lactate and modulation of short chain fatty acid absorption from the reticulorumen of non-lactating cattle fed high concentrate diets. PLoS One 11, e0164192.CrossRefGoogle ScholarPubMed
Rodríguez-Lecompte, JC, Kroeker, AD, Ceballos-Márquez, A, Li, S, Plaizier, JC and Gomez, DE 2014. Evaluation of the systemic innate immune response and metabolic alterations of non lactating cows with diet-induced subacute ruminal acidosis. Journal of Dairy Science 97, 7777–7787.CrossRefGoogle Scholar
Steiger, M, Senn, M, Altreuther, G, Werling, D, Sutter, F, Kreuzer, M and Langhans, W 1999. Effect of prolonged low-dose lipopolysaccharide infusion on feed intake and metabolism in heifers. Journal of Animal Science 77, 2523–2532.CrossRefGoogle ScholarPubMed
Waldron, MR, Nishida, T, Nonnecke, BJ and Overton, TR 2003a. Effect of lipopolysaccharide on indices of peripheral and hepatic metabolism in lactating cows. Journal of Dairy Science 86, 3447–3459.CrossRefGoogle ScholarPubMed
Waldron, MR, Nonnecke, BJ, Nishida, T, Horst, RL and Overton, TR 2003b. Effect of lipopolysaccharide infusion on serum macromineral and vitamin D concentration in dairy cows. Journal of Dairy Science 86, 3440–3446.CrossRefGoogle ScholarPubMed
Wille, S, Simon, A, Platen, M and Oertel, C 2010. Factors influencing the activity of liver enzymes of clinically healthy dairy cows under field conditions. Züchtungskunde 82, 155–164.Google Scholar
Zebeli, Q and Metzler-Zebeli, BU 2012. Interplay between rumen digestive disorders and diet-induced inflammation in dairy cattle. Research in Veterinary Science 93, 1099–1108.CrossRefGoogle ScholarPubMed
Zuur, AF, Ieno, EN and Elphick, CS 2010. A protocol for data exploration to avoid common statistical problems. Methods in Ecology and Evolution 1, 3–14.CrossRefGoogle Scholar
Supplementary material: File

Aditya et al supplementary material

Table S1

Download Aditya et al supplementary material(File)
File 14.2 KB