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Effects of cloned-cattle meat on reproductive physiology in rats

Published online by Cambridge University Press:  19 October 2009

S. Hwang ,
N.-J. Lee ,
J.-S. Hwang ,
B.-C. Yang ,
G.-S. Im ,
Y.-G. Ko ,
E.-W. Park ,
S.-B. Park ,
J.-K. Kang  and
H.-H. Seong
S. Hwang
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
N.-J. Lee
Affiliation:
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, 12 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea
J.-S. Hwang
Affiliation:
Biotoxtech Co. Ltd, 58-1, Ochang Science Industrial Complex, Cheongwon, Chungbuk 363-883, Korea
B.-C. Yang
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
G.-S. Im
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
Y.-G. Ko
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
E.-W. Park
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
S.-B. Park
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
J.-K. Kang*
Affiliation:
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, 12 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea Biotoxtech Co. Ltd, 58-1, Ochang Science Industrial Complex, Cheongwon, Chungbuk 363-883, Korea
H.-H. Seong*
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, RDA, 77 Chuksan-gil, Suwon, Gyeonggi 441-706, Korea
*
†E-mail: jkkang@chungbuk.ac.kr
seonghh@rda.go.kr
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Abstract

The influence of the cloned-cattle meat diets upon reproduction in mammals was rarely studied. This study was performed to analyze the effects of the diets containing cloned-cattle (Korean native beef, Hanwoo) meat on the reproductive physiology in rats. The male and female rats were fed with the diets containing 5% or 10% of normal- (N-5 or N-10) or cloned- (C-5 or C-10) cattle meat during test periods. The rats fed with commercial pellets were used as control. Lower food consumption in normal- and cloned-cattle meat diet groups is detected in both male and female rats compared with that of control (P < 0.05, 0.01 and 0.001). No signs of cloned-cattle meat diets on male reproductive parameters are found in all groups, except for lower sperm deformity in C-5 group (P < 0.05) and higher testosterone concentration in C-10 group (P < 0.05), respectively. There are no significant test substance-related differences of Caesarean section and delivery in dams and external examination and physiological development test in neonate compared with control and normal meat groups. Based on these results, it can be postulated that there are no obvious negative effects on the reproductive physiology in rats fed with cloned-cattle meat diets compared to their comparators.

Keywords

animal cloningcloned-cattle meat dietratsreproductive parametersrisk assessment
Type
Full Paper
Information
animal , Volume 4 , Issue 2 , February 2010 , pp. 218 - 223
Copyright
Copyright © The Animal Consortium 2009

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References

Animal Biotechnology: Science-based Concerns. 2002. The National Research Council, The National Academy Press (http://www.nap.edu/catalog/10418.html), Washington DC, pp. 61–72.Google Scholar
Baguisi, A, Behboodi, E, Melican, DT, Pollock, JS, Destrempes, MM, Cammuso, C, Williams, JL, Nims, SD, Porter, CA, Midura, P, Palacios, MJ, Ayres, SL, Denniston, RS, Hayes, ML, Ziomek, CA, Meade, HM, Godke, RA, Gavin, WG, Overstrom, EW, Echelard, Y 1999. Production of goats by somatic cell nuclear transfer. Nature Biotechnology 17, 456–461.CrossRefGoogle ScholarPubMed
Dunnett, CW 1980. Pairwise multiple comparisons in the unequal variance case. Journal of the American Statistical Association 75, 796–800.CrossRefGoogle Scholar
Enright, BP, Taneja, M, Schreiber, D, Riesen, J, Tian, XC, Fortune, JE, Yang, X 2002. Reproductive characteristics of cloned heifers derived from adult somatic cells. Biology of Reproduction 66, 291–296.CrossRefGoogle ScholarPubMed
European Food Safety Society Scientific Committee 2008. Food Safety, Animal health and welfare and environmental impact of animals derived from cloning by somatic cell nucleus transfer (SCNT) and their offspring and products obtained from those animals. The EFSA Journal 767, 1–49.Google Scholar
Galli, C, Duchi, R, Lagutina, I, Lazzari, G 2004. A European perspective on animal cloning and government regulation. IEEE Engineering in Medicine and Biology Magazine 23, 52–54.CrossRefGoogle ScholarPubMed
Govoni, KE, Tian, XC, Kazmer, GW, Taneja, M, Enright, BP, Rivard, AL, Yang, X, Zinn, SA 2002. Age-related changes of the somatotropic axis in cloned Holstein calves. Biology of Reproduction 66, 1293–1298.CrossRefGoogle ScholarPubMed
Hwang, S, Chang, YM, Ko, YG, Yang, BC, Min, KS, Yoon, JT, Nho, WG, Kim, CK, Seong, HH 2009. Delayed parturition in cloned calves associated with persistently elevated placentomal TGF-beta(1) expression. Animal Reproduction Science 115, 290–295.CrossRefGoogle Scholar
Kang, YK, Koo, DB, Park, JS, Choi, YH, Chung, AS, Lee, KK, Han, YM 2001. Aberrant methylation of donor genome in cloned bovine embryos. Nature Genetics 28, 173–177.CrossRefGoogle ScholarPubMed
Kato, Y, Tani, T, Sotomaru, Y, Kurokawa, K, Kato, J, Doguchi, H, Yasue, H, Tsunoda, Y 1998. Eight calves cloned from somatic cells of a single adult. Science 282, 2095–2098.CrossRefGoogle ScholarPubMed
Kelly, L 2005. The safety assessment of foods from transgenic and cloned animals using the comparative approach. Revue Scientifique et Technique (International Office of Epizootics) 24, 61–74.Google ScholarPubMed
Lanza, RP, Cibelli, JB, Faber, D, Sweeney, RW, Henderson, B, Nevala, W, West, MD, Wettstein, PJ 2001. Cloned cattle can be healthy and normal. Science 294, 1893–1894.CrossRefGoogle ScholarPubMed
Mylchreest, E, Malley, LA, O’neill, AJ, Kegelman, TA, Sykes, GP, Valentine, R 2006. Reproductive and developmental toxicity of inhaled 2,3-dichloro-1,3-butadiene in rats. Reproductive Toxicology 22, 613–622.CrossRefGoogle ScholarPubMed
Polejaeva, IA, Chen, SH, Vaught, TD, Page, RL, Mullins, J, Ball, S, Dai, Y, Boone, J, Walker, S, Ayares, DL, Colman, A, Campbell, KH 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407, 86–90.CrossRefGoogle ScholarPubMed
Rudenko, L, Matheson, JC, Adams, AL, Dubbin, ES, Greenlees, KJ 2004. Food consumption risks associated with animal clones: what should be investigated? Cloning and Stem Cells 6, 79–93.CrossRefGoogle ScholarPubMed
Safety of Genetically Engineered Foods Approaches to Assessing Unidentified Health Effects 2004. The National Research Council, The National Academy Press (http://www.nap.edu/catalog/10977.html), Washington DC, pp. 217–236.Google Scholar
Savage, AF, Maull, J, Tian, XC, Taneja, M, Katz, L, Darre, M, Yang, X 2003. Behavioral observations of adolescent Holstein heifers cloned from adult somatic cells. Theriogenology 60, 1097–1110.CrossRefGoogle ScholarPubMed
Takahashi, S, Ito, Y 2004. Evaluation of meat products from cloned cattle: biological and biochemical properties. Cloning and Stem Cells 6, 165–171.CrossRefGoogle ScholarPubMed
Tian, XC, Kubota, C, Sakashita, K, Izaike, Y, Okano, R, Tabara, N, Curchoe, C, Jacob, L, Zhang, Y, Smith, S, Bormann, C, Xu, J, Sato, M, Andrew, S, Yang, X 2005. Meat and milk compositions of bovine clones. Proceedings of the National Academy of Sciences of the United States of America 102, 6261–6266.CrossRefGoogle ScholarPubMed
Tian, XC, Xu, J, Yang, X 2000. Normal telomere lengths found in cloned cattle. Nature Genetics 26, 272–273.CrossRefGoogle ScholarPubMed
Tome, D, Dubarrym, M, Fromentin, G 2004. Nutritional value of milk and meat products derived from cloning. Cloning and Stem Cells 6, 172–177.CrossRefGoogle ScholarPubMed
Walsh, MK, Lucey, JA, Govindasamy-Lucey, S, Pace, MM, Bishop, MD 2003. Comparison of milk produced by cows cloned by nuclear transfer with milk from non-cloned cows. Cloning and Stem Cells 5, 213–219.CrossRefGoogle ScholarPubMed
Wilmut, I, Schnieke, AE, McWhir, J, Kind, AJ, Campbell, KH 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813.CrossRefGoogle ScholarPubMed
Yamaguchi, M, Ito, Y, Takahashi, S 2007. Fourteen-week feeding test of meat and milk derived from cloned cattle in the rat. Theriogenology 67, 152–165.CrossRefGoogle ScholarPubMed
Yang, BC, Im, GS, Kim, DH, Yang, BS, Oh, HJ, Park, HS, Seong, HH, Kim, SW, Ka, HH, Lee, CK 2008. Development of vitrified-thawed bovine oocytes after in vitro fertilization and somatic cell nuclear transfer. Animal Reproduction Science 103, 25–37.CrossRefGoogle ScholarPubMed
Yang, X, Tian, XC, Kubota, C, Page, R, Xu, J, Cibelli, J, Seidel, G 2007. Risk assessment of meat and milk from cloned animals. Nature Biotechnology 25, 77–83.CrossRefGoogle ScholarPubMed
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