Hostname: page-component-84b7d79bbc-7nlkj Total loading time: 0 Render date: 2024-07-30T02:50:39.291Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Published online by Cambridge University Press:  17 December 2013

F. Turri ,
M. Madeddu ,
T. M. Gliozzi ,
G. Gandini  and
F. Pizzi
F. Turri
Affiliation:
Institute of Agricultural Biology and Biotechnology, Lodi Unit, National Research Council, c/o Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy
M. Madeddu
Affiliation:
Institute of Agricultural Biology and Biotechnology, Lodi Unit, National Research Council, c/o Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy
T. M. Gliozzi
Affiliation:
Institute of Agricultural Biology and Biotechnology, Lodi Unit, National Research Council, c/o Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy
G. Gandini
Affiliation:
Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy
F. Pizzi*
Affiliation:
Institute of Agricultural Biology and Biotechnology, Lodi Unit, National Research Council, c/o Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy
*
†E-mail: pizzi@ibba.cnr.it
Get access

Abstract

The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal’s death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n=50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n=50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

Keywords

goatepididymal spermcryopreservationgenebanking
Type
Full Paper
Information
animal , Volume 8 , Issue 3 , March 2014 , pp. 440 - 447
Copyright
© The Animal Consortium 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Amann, RP, Seidel, GE Jr and Mortimer, RG 2000. Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet. Theriogenology 54, 1499–1515.CrossRefGoogle ScholarPubMed
Bagirov, V, Nasibov, SH and Klenovitskii, P 2009. Animal gene pool preservation and conservation. Russian Agriculture Sciences 35, 112–115.CrossRefGoogle Scholar
Bakst, MR and Cecil, HC 1997. Sperm viability. I. Nigrosin/Eosin stain for determining live/dead and abnormal sperm counts. In Techniques for semen evaluation, semen storage and fertility determination (ed. MR Bakst and HC Cecil), pp 29–31. Poultry Science Association, Savoy, IL.Google Scholar
Bisset, C and Bernard, RTF 2005. The effect of prolonged cold storage of eland (Taurotragus oryx) cauda epididymides on the spermatozoa: possible implications for the conservation of biodiversity. Theriogenology 63, 1592–1604.CrossRefGoogle Scholar
Blash, S, Melican, D and Gavin, W 2000. Cryopreservation of epididymal sperm obtained at necroscopsy from goats. Theriogenology 54, 899–905.CrossRefGoogle ScholarPubMed
Chatizaa, FP, Bartelsb, P, Nedambalec, TL and Wagenaara, GM 2012. Computer assisted sperm analysis of motility patterns of postthawed epididymal spermatozoa of springbok (Antidorcas marsupialis), impala (Aepyceros melampus), and blesbok (Damaliscus dorcus phillipsi) incubated under conditions supporting domestic cattle in vitro fertilization. Theriogenology 78, 402–414.Google Scholar
Ehling, C, Rath, D, Struckmann, C, Frenzel, A, Schindler, L and Niemann, H 2006. Utilization of frozen–thawed epididymal ram semen to preserve genetic diversity in Scrapie susceptible sheep breeds. Theriogenology 66, 2160–2164.Google Scholar
Farrell, PB, Presicce, GA, Brockett, CC and Foote, RH 2000. Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology 49, 871–879.Google Scholar
Fawcett, DW and Phillips, DM 1969. Observations on the release of spermatozoa and on changes in the head during passage through the epididymis. Journal of Reproduction and Fertility 17 (suppl. 6), 405–418.Google Scholar
Fernández-Santos, MR, Soler, AJ, Ramón, M, Ros-Santaella, JL and Maroto-Morales, A 2011. Effect of post mortem time on post-thaw characteristics of Spanish ibex (Capra pyrenaica) spermatozoa. Animal Reproduction Science 129, 56–66.Google Scholar
Garner, DL and Johnson, LA 1995. Viability assessment of mammalian sperm using SYBR-14 and Propidium Iodide. Biology of Reproduction 53, 276–284.CrossRefGoogle ScholarPubMed
James, AN, Green, H, Hoffman, S, Landry, AM, Paccamonti, D and Godke, RA 2002. Preservation of equine sperm stored in the epididymis at 4°C for 24, 48, 72 and 96 hours. Theriogenology 58, 401–404.Google Scholar
Kaabi, M, Paz, P, Alvarez, M, Anel, E, Boixo, JC, Rouissi, H, Herraez, P and Anel, L 2003. Effect of epididymis handling conditions on the quality of ram spermatozoa recovered post mortem. Theriogenology 60, 1249–1259.CrossRefGoogle ScholarPubMed
Kathiravan, P, Kalatharan, J, Edwin, MJ and Veerapandian, C 2008. Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes. Animal Reproduction Science 104, 9–17.CrossRefGoogle ScholarPubMed
Kikuchi, K, Nagai, J, Kashiwazaki, N, Ikeda, H, Noguchi, J, Shimada, A, Soloy, E and Kaneko, H 1998. Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4°C. Theriogenology 50, 615–623.CrossRefGoogle Scholar
Killian, I, Lubbe, K, Bartels, P, Friedmann, Y and Denniston, RS 2000. Evaluating epididymal sperm of African wild ruminants: longevity when stored at 4°C and viability following cryopreservation. Theriogenology 53, 336.Google Scholar
León-Quinto, T, Simón, MA, Cadenas, R, Jones, J, Martínez-Hernández, F, Moreno, JM, Vargas, A, Martínez, F and Soria, B 2009. Developing biological resource banks as a supporting tool for wildlife reproduction and conservation. Animal Reproduction Science 112, 347–361.Google Scholar
Lone, FA, Islam, R, Khan, MZ and Sofi, KA 2011. Effect of transportation temperature on the quality of cauda epididymal spermatozoa of ram. Animal Reproduction Science 123, 54–59.Google Scholar
Martins, CF, Driessen, K, Melo Costa, P, Carvalho-Neto, JO, de Sousa, RV, Rumpf, R and Dode, MN 2009. Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5°C by different periods of time. Animal Reproduction Science 116, 50–57.CrossRefGoogle Scholar
Nichi, M, Goovaerts, IGF, Cortada, CNM, Barnabe, VH, de Clercq, JBP and Bols, PEJ 2007. Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34°C. Theriogenology 67, 334–340.Google Scholar
Robaire, B and Hermo, L 1994. Efferent ducts, epididymis and vas deferens: structure, function and regulation. In The physiology of reproduction (ed. E Knobil and JD Neill), pp 999–1080. Raven Press, New York.Google Scholar
Salamon, S and Maxweel, WM 2000. Storage of ram semen. Animal Reproduction Science 62, 77–111.Google Scholar
Santiago-Moreno, J, Toledano-Díaz, A, Pulido-Pastor, A, Gómez-Brunet, A and López-Sebastián, A 2006. Birth of live Spanish ibex (Capra pyrenaica hispanica) derived from artificial insemination with epididymal spermatozoa retrieved after death. Theriogenology 66, 283–291.Google Scholar
Santiago-Moreno, J, Astorga, RJ, Luque, I, Coloma, AM, Toledano-Dıaz, A, Pulido-Pastor, A, Gomez-Guillamon, F, Salas-Vega, R and Lopez-Sebastian, A 2009. Influence of recovery method and microbial contamination on the response to freezing–thawing in ibex (Capra pyrenaica) epididymal spermatozoa. Cryobiology 59, 357–362.Google Scholar
Soler, AJ, Perez-Guzman, MD and Garde, JJ 2003. Storage of red deer epididymides for four days at 5°C: effects on sperm motility, viability, and morphological integrity. Journal of Experimental Zoology Part A: Comparative Experimental Biology 295, 188–199.Google Scholar
Songsasen, N, Tong, J and Leibo, S 1998. Birth of live mice derived by in vitro fertilization with spermatozoa retrieved up to twenty-four hours after death. Journal of Experimental Zoology 280, 189–196.Google Scholar
Turri, F, Madeddu, M, Gliozzi, TM, Gandini, G and Pizzi, F 2012. Influence of recovery methods and extenders on bull epididymal spermatozoa quality. Reproduction in Domestic Animals 47, 712–717.Google Scholar