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Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Published online by Cambridge University Press:  15 March 2010

A. Herdmann ,
K. Nuernberg ,
J. Martin ,
G. Nuernberg  and
O. Doran
A. Herdmann
Affiliation:
Unit Muscle Biology and Growth, FBN Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
K. Nuernberg
Affiliation:
Unit Muscle Biology and Growth, FBN Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
J. Martin
Affiliation:
State Institute for Agriculture and Fishing Research, Institute for Animal Production, 18196 Dummerstorf, Germany
G. Nuernberg
Affiliation:
Unit Muscle Biology and Growth, FBN Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
O. Doran*
Affiliation:
School of Life Sciences, University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol BS16 1 QY, UK
*
†E-mail: Olena.Doran@uwe.ac.uk
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Abstract

This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 desaturase (Δ6d) in longissimus muscle and subcutaneous adipose tissue of bulls. Supplementation of the diet with C18:3n-3 was accompanied by an increased level of n-3 fatty acids in muscle which resulted in decrease of n-6/n-3 ratio. The diet enriched with n-3 polyunsaturated fatty acids (PUFAs) significantly inhibited SCD protein expression in muscle and subcutaneous adipose tissue, and reduced the Δ6d expression in muscle. There was no significant effect of the diet on ACC protein expression. Inhibition of the Δ6d expression was associated with a decrease in n-6 PUFA level in muscles, whereas repression of SCD protein was related to a lower oleic acid (C18:1 cis-9) content in the adipose tissue. Expression of ACC, SCD and Δ6d proteins was found to be relatively higher in subcutaneous adipose tissue when compared with longissimus muscle. It is suggested that dietary manipulation of fatty acid composition in ruminants is mediated, at least partially, through the regulation of lipogenic enzymes expression and that regulation of the bovine lipogenic enzymes expression is tissue specific.

Keywords

cattledietenzyme expressionfatty acidlipogenic enzyme
Type
Full Paper
Information
animal , Volume 4 , Issue 5 , May 2010 , pp. 755 - 762
Copyright
Copyright © The Animal Consortium 2010

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