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Effect of detoxified karanja (Pongamia spp.) cake on testicular architecture and semen production in ram lambs

Published online by Cambridge University Press:  19 July 2013

D. Dineshkumar ,
S. Selvaraju ,
S. Parthipan ,
Allen Thayakumar ,
D. Rajendran ,
J. P. Ravindra ,
P. Krishnamoorthy ,
I. J. Reddy  and
S. B. N. Rao
D. Dineshkumar
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. Selvaraju*
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. Parthipan
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
Allen Thayakumar
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
D. Rajendran
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
J. P. Ravindra
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
P. Krishnamoorthy
Affiliation:
Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560024, India
I. J. Reddy
Affiliation:
Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
S. B. N. Rao
Affiliation:
Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
*
†E-mail: selvarajuars@redifmail.com
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Abstract

The protein-rich non-conventional detoxified karanja cake (dKC) can be used in place of conventional protein supplements like soybean meal (SBM), groundnut meal, etc. in livestock feed. The present study was conducted to assess the effect of two levels of dKC by replacing SBM on testicular architecture, semen quality and expressions of mRNAs encoding luteinizing hormone receptor (LHR) and insulin-like growth factor (IGF-I) in testes of ram lambs. Eighteen ram lambs were randomly divided into three groups (n = 6) and fed different levels (%) of karanja cake (0% replacement – control; 50% replacement – dKC-50 and 75% replacement – dKC-75) for 140 days. After 120 days of feeding, the semen from the animals was collected and analysed. The testes samples were collected on day 140 of feeding for transcripts expression studies. The dKC-50 group had no change in BW, whereas dKC-75 group showed decreased (P < 0.05) BW as compared with control. The number of animals ejaculated semen in dKC-75 group was lower (P < 0.05) than the control group. A reduction (P < 0.05) in LHR expression in dKC-75 was observed, whereas a reduction in IGF-I expression (P < 0.05) was observed in dKC-50 and dKC-75 as compared with control group. The study reveals that in ram lambs, long-term feeding of dKC at 50% replacement of SBM may not affect BW. However, long-term feeding of dKC as a replacement of SBM may affect testicular function.

Keywords

karanja cakeram lambssemen qualityIGF-ILHR
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 10 , October 2013 , pp. 1697 - 1703
Copyright
Copyright © The Animal Consortium 2013 

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