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A protocol for the cryoconservation of breeds by low-cost emergency cell banks – a pilot study

Published online by Cambridge University Press:  01 January 2008

E. Groeneveld*
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Huu Tinh
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
W. Kues
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Thi Vien
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
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Abstract

A protocol was developed for collection and storage of somatic cell samples under adverse conditions with little infrastructure, for somatic-cell banks as a backup for endangered livestock breeds. The procedure, which is uniform across species, includes sample collection with ear taggers with an integrated tag/vial system, and recording of global positioning system (GPS) coordinates, digital images and breed information. In a pilot study, the procedures were tested on six local Vietnamese populations of pigs, sheep and goats. Initial investment was around €3000, while the total variable cost for sampling one breed with 25 females and 25 males was less than €1000. With support from local organisations, the sampling of six breeds with 300 animals was carried out in 2 months. The protocol and the complete workflow for setting up a somatic-cell bank, together with data collection, are described. The procedure has proved practicable and exceedingly cheap relative to the cryopreservation of semen or embryos.

Type
Full Paper
Copyright
Copyright © The Animal Consortium 2008

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