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Effect of type of diet (casein-based or protein-free) and caecotrophy on ileal endogenous nitrogen and amino acid flow in rabbits

Published online by Cambridge University Press:  18 August 2016

A. I. García
Affiliation:
Departamento de Producciόn Animal, ETS Ingenieros Agronomos, Universidad Politécnica 28040, Madrid, Spain
J. C. de Bias
Affiliation:
Departamento de Producciόn Animal, ETS Ingenieros Agronomos, Universidad Politécnica 28040, Madrid, Spain
R. Carabaño*
Affiliation:
Departamento de Producciόn Animal, ETS Ingenieros Agronomos, Universidad Politécnica 28040, Madrid, Spain
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Abstract

Twenty-eight New Zealand White X Californian doe rabbits weighing 3791±141 g were surgically fitted with a glass T-cannula at ileum level. Animals were given ad libitum access to either a casein-based diet (C) or a protein-free diet (PF). The only difference in the ingredient composition of the diets was the substitution of 160g/kg of maize starch by casein in the C diet. Animals were randomly allotted to each experimental diet. Seven rabbits per diet were not allowed to practice caecotrophy. The C diet resulted in higher food dry matter (DM) intake (DMI) (111-5 v. 51-5 g/day), food nitrogen intake (3-12 v. 0-20 g/day), ileal flow of DM (56-0 v. 23-8 g/day) and ileal flow of nitrogen (0-72 v. 0-24 g/day) but lower soft faeces DMI (21-7 v. 41-4 g/day) than the PF diet. A linear relationship between ileal endogenous nitrogen flow and total DMI was found for animals given the C but not for the PF diet. The ingestion ofC diet led to a higher (proportionately 0-41, on average), endogenous ileal amino acid flow than PF diet for all amino acids except for glycine. The ileal flow of glycine was proportionately 0-25 higher in animals given the PF diet than in animals given the C diet. The C diet resulted in higher content of nitrogen, threonine and proline in soft faeces than the PF diet. In contrast, the ingestion of the PF diet led to a higher content of lysine and methionine in soft faeces than the C diet. Within the C diet, animals practising caecotrophy showed higher ileal flow of DM (73-4 v. 56-0 g/day) and phenylalanine (0-834 v. 0-677 g/kg DMI) than animals not practising it. Caecotrophy led to a higher endogenous proportion of some of the most important limiting essential amino acids in rabbits, such as arginine, lysine, phenylalanine and threonine. In rabbits, the use of PF diets has limited practical application in the estimation of nitrogen endogenous losses as the low food DMI and the high soft faecesDMI leads to abnormal food: soft faeces intake ratio. Moreover, due to the important effect of caecotrophy on the amino acid composition of endogenous losses, it is important to correct the total amino acid content of the ileal chyme by the undigested amino acid residue coming from the soft faeces intake. This correction could be made, without the methodological complexity implied, by fitting animals with a wooden collar and thereby avoiding caecotrophy as in the present trial.

Type
Non-ruminant nutrition, behaviour and production
Copyright
Copyright © British Society of Animal Science 2004

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References

Association of Official Analytical Chemists.1995. Official methods of analysis, 16th edition. Association of Official Analytical Chemists, Arlington, VA.Google Scholar
Bias, E., Falcao, L., Gidenne, T., Pinheiro, V., Garcia, A. I. and Carabafio, R. 2000. Measurement of ileal digestibility in rabbits: an inter-laboratory study to compare two markers and two frequencies of digesta collections. World Rabbit Science 8: (suppl.) 131137.Google Scholar
Bias, J. C. de and Mateos, G. G. 1998. Feed formulation. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 241253. Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Boisen, S. and Moughan, P. J. 1996. Dietary influences on endogenous ileal protein and amino acid loss in the pig — a review. Acta Agricultural Scandinavica 46:154164.Google Scholar
Butts, C. A., Moughan, P. J., Smith, W. C. and Carr, D. H. 1993b. Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): the effect of protein-free, synthetic amino acid, peptide and protein alimentation Journal of the Science of Food and Agriculture 61: 3140.CrossRefGoogle Scholar
Butts, C. A., Moughan, P. J., Smith, W. C., Reynolds, G. W. and Garrick, D. J. 1993a. The effect of feed dry matter intake on endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig. journal of the Science of Food and Agriculture 62: 235243.Google Scholar
Carabafio, R., Fraga, M. J., Santoma, G. and Bias, J. C. de. 1988. Effect of diet on composition of cecal contents and on excretion and composition of soft faeces and hard faeces of rabbits. Journal of Animal Science 66: 901910.CrossRefGoogle Scholar
Carabafio, R. and Piquer, J. 1998. The digestive system of the rabbit. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 116. Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Donkoh, A. and Moughan, P. J. 1999. Endogenous ileal nitrogen and amino acid flows in the growing pig receiving a protein-free diet and diets containing enzymically hydrolysed casein or graded levels of meat and bone meal. Animal Science 68:511518.Google Scholar
Donkoh, A., Moughan, P. J. and Morel, P. C. H. 1995. Comparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat. Journal of the Science of Food and Agriculture 67: 359366.Google Scholar
Fraga, M. J. 1998. Protein digestion. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 3953. Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Fraga, M. J., Peréz de Ayala, P., Carabafio, R. and Bias, J. C. de. 1991. Effect of type of fibre on the rate of passage and on the contribution of soft faeces to nutrient intake of finishing rabbits. Journal of Animal Science 69:15661574.Google Scholar
Garcίa, J., Carabaño, R., Péréz-Alba, L. and Bias, J. C. de. 2000. Effect of fibre source on cecal fermentation and nitrogen recycled through caecotrophy in rabbits. Journal of Animal Science 78: 638646.CrossRefGoogle Scholar
Gidenne, T., Bouyssou, T. and Ruckebusch, Y. 1988. Sampling of digestive contents by ileal cannulation in the rabbit. Animal Production 46:147151.Google Scholar
Gidenne, T., Carabafio, R., Garίia, J. and Bias, J. C. de. 1998. Fibre digestion. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 6988, Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Hagemeister, H. and Erbersdobler, H. 1985. Chemical labelling of dietary protein by transformation of lysine to homoarginine: a new technique to follow intestinal digestion and absorption. Proceedings of the Nutrition Society 44:133A.Google Scholar
Hess, V. and Seve, B. 1999. Effects of body weight and feed intake level on basal ileal endogenous losses in growing pigs. Journal of Animal Science 77: 32813288.Google Scholar
Hodgkinson, S. M., Moughan, P. J. and Reynolds, G. W. 2000a. Effect of duration of feeding a protein-free diet on endogenous ileal nitrogen and amino acid loss in the growing pig. Journal of the Science of Food and Agriculture 80: 14071412.Google Scholar
Hodgkinson, S. M., Moughan, P. J., Reynolds, G. W. and James, K. A. C. 2000b. The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig. British Journal of Nutrition 83: 421430.Google Scholar
Hornicke, H. and Bjornhag, G. 1980. Caecotrophy and related strategies for digesta utilization. In Digestive physiology and metabolism in ruminant (ed. Ruckebusch, Y. and Thivend, P.), pp. 707730. MTP Press, Lancaster.Google Scholar
Jansman, A. J. M., Smink, W., Leeuwen, P. and Rademacher, M. 2002. Evaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Animal Feed Science and Technology 98:4960.Google Scholar
Lange, C. F. M. de, Sauer, W. C., Moshenthin, R. and Souffrant, W. B. 1989. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and faeces in pigs. Journal of Animal Science 67: 746754.Google Scholar
Lebas, F., Gidenne, T., Peréz, J. M. and Licois, D. 1998. Nutrition and pathology. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 197214, Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Licitra, G., Hernandez, T. M. and Van Soest, P. J. 1996. Standardization of procedures for nitrogen fractionation of ruminant feed. Animal Feed Science and Technology 57: 347358.Google Scholar
Nicodemus, N., Mateos, J., Bias, J. C. de, Carabafio, R. and Fraga, M. J. 1999. Effect of diet on amino acid composition of soft faeces and the contribution of soft faeces to total amino acid intake, through caecotrophy in lactating doe rabbits. Animal Science 69:167170.Google Scholar
Parigi Bini, R. and Xiccato, G. 1998. Energy metabolism and requirements. In The nutrition of the rabbit (ed. de Bias, J. C. and Wiseman, J.), pp. 103131, Commonwealth Agricultural Bureaux, Wallingford, UK.Google Scholar
Sauer, W. C. and Ozimek, L. 1986. Digestibility of amino acids in swine: results and their practical application. A review. Livestock Production Science 15:367388.Google Scholar
Schneeman, B. O. 1982. Digestive enzyme activities from the pancreas in response to diet. In Digestive physiology in the pig (ed. Laplace, J. P., Corring, T. and Rerat, A.), second international seminar Jouy-en-Josas-Versailles, France, 1982. Les colloques I'INRA no. 12, pp. 125131. Institute National de la Recherche Agronomique, Paris.Google Scholar
Schulze, H., Leeuwen, P.van, Verstegen, M. W. A.., Huisman, J., Souffrant, W. B. and Ahrens, F. 1994. Effect of level of dietary neutral detergent fibre on ileal apparent digestibility and ileal nitrogen losses in pigs. Journal of Animal Science 72:23622368.Google Scholar
Snedecor, G. W. and Cochran, W. G. 1989. Statistical methods, 8th edition. Iowa State University Press, Ames, I A.Google Scholar
Souffrant, W. B., Fevrier, C., Laplace, J. P. and Hennig, U. 1997. Comparison of methods to estimate ileal endogenous nitrogen and amino acids in piglets. Proceedings of the Vllth international symposium on digestive physiology in pigs (ed. Laplace, J. P., Fevrier, C. and Barbeau, A.), EAAP publication no. 88, pp. 591595. Institute National de la Recherche Agronomique, Saint Malo, France.Google Scholar
Spanish Royal Decree 223/88. 1988. Sobre protectiόn de los animals utilizados para experimentatiόn y otros fines cientificos. Boletin Oficial del Estado no. 67, pp. 85098511.Google Scholar
Spreadbury, D. 1978. A study on the protein and amino acid requirements of growing New Zealand White rabbit with emphasis on lysine and sulfur-containing amino acids. British Journal of Nutrition 39:601613.Google Scholar
Statistical Analysis Systems Institute. 1990. User's guide, version 6, fourth edition. SAS Institute Inc., Cary, NC. Google Scholar
Tanksley, F. D. and Knabe, D. A. 1984. Ileal digestibilities of amino acids in pig feeds and their use in formulating diets. In Recent advances in animal nutrition (ed. Haresign, W. and Cole, D. J. A.), pp. 7595. Butterworths, London.Google Scholar
Uden, P., Colucci, E. P. and Van Soest, J. P. 1980. Investigation of ytterbium, cerium and cobalt as markers in digesta. Rate of passage studies. Journal of the Science of Food and Agriculture 31:625632.Google Scholar
Van Soest, J. P., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fibre, neutral detergent fibre and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 35833597.Google Scholar
Zebrowska, T. 1973. Digestion and absorption of nitrogenous compounds in the large intestine of pigs. Roczniki Nauk Rolniczych B95: 8590.Google Scholar