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Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows

Published online by Cambridge University Press:  18 August 2016

K. J. Shingfield ,
S. Ahvenjärvi ,
V. Toivonen ,
A. Ärölä ,
K. V. V. Nurmela ,
P. Huhtanen  and
J. M. Griinari
K. J. Shingfield*
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, FIN 31600, Jokioinen, Finland
S. Ahvenjärvi
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, FIN 31600, Jokioinen, Finland
V. Toivonen
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, FIN 31600, Jokioinen, Finland
A. Ärölä
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, FIN 31600, Jokioinen, Finland
K. V. V. Nurmela
Affiliation:
Valio Limited, FIN-00039, Helsinki, Finland
P. Huhtanen
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, FIN 31600, Jokioinen, Finland
J. M. Griinari
Affiliation:
Department of Animal Science, University of Helsinki, Helsinki, Finland
*
†Present address: School of Food Biosciences, University of Reading, PO Box 226, Reading RG6 6AP, UK. E-mail:K.J.Shingfield@reading.ac.uk
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Abstract

Mechanisms underlying milk fat conjugated linoleic acid (CLA) responses to supplements of fish oil were investigated using five lactating cows each fitted with a rumen cannula in a simple experiment consisting of two consecutive 14-day experimental periods. During the first period cows were offered 18 kg dry matter (DM) per day of a basal (B) diet formulated from grass silage and a cereal based-concentrate (0·6 : 0·4; forage : concentrate ratio, on a DM basis) followed by the same diet supplemented with 250 g fish oil per day (FO) in the second period. The flow of non-esterified fatty acids leaving the rumen was measured using the omasal sampling technique in combination with a triple indigestible marker method based on Li-Co-EDTA, Yb-acetate and Cr-mordanted straw. Fish oil decreased DM intake and milk yield, but had no effect on milk constituent content. Milk fat trans-11 C18:1, total trans-C18 : 1, cis-9 trans-11 CLA, total CLA, C18 : 2 (n-6) and total C18 : 2 content were increased in response to fish oil from 1·80, 4·51, 0·39, 0·56, 0·90 and 1·41 to 9·39, 14·39, 1·66, 1·85, 1·25 and 4·00 g/100 g total fatty acids, respectively. Increases in the cis-9, trans-11 isomer accounted for proportionately 0·89 of the CLA response to fish oil. Furthermore, fish oil decreased the flow of C18 : 0 (283 and 47 g/day for B and FO, respectively) and increased that of trans-C18 : 1 fatty acids entering the omasal canal (38 and 182 g/day). Omasal flows of trans-C18 : 1 acids with double bonds in positions from delta-4 to -15 inclusive were enhanced, but the effects were isomer dependent and primarily associated with an increase in trans-11 C18 : 1 leaving the rumen (17·1 and 121·1 g/day for B and FO, respectively). Fish oil had no effect on total (4·36 and 3·50 g/day) or cis-9, trans-11 CLA (2·86 and 2·08 g/day) entering the omasal canal. Flows of cis-9, trans-11 CLA were lower than the secretion of this isomer in milk. Comparison with the transfer of the trans-9, trans-11 isomer synthesized in the rumen suggested that proportionately 0·66 and 0·97 of cis-9, trans-11 CLA was derived from endogenous conversion of trans-11 C18 : 1 in the mammary gland for B and FO, respectively. It is concluded that fish oil enhances milk fat cis-9, trans-11 CLA content in response to increased supply of trans-11 C18:1 that arises from an inhibition of trans-C18 : 1 reduction in the rumen.

Keywords

biohydrogenationconjugated linoleic acidmilk fatpolyenoic fatty acidstrans fatty acids
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 77 , Issue 1 , August 2003 , pp. 165 - 179
Copyright
Copyright © British Society of Animal Science 2003

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References

Ahvenjärvi, S., Vanhatalo, A., Huhtanen, P. and Varvikko, T. 2000. Determination of reticulo-rumen and whole-stomach digestion in lactating cows by omasal canal or duodenal sampling. British Journal of Nutrition 83: 67–77.CrossRefGoogle ScholarPubMed
Baumgard, L. H., Corl, B.A., Dwyer, D.A. and Bauman, D.E. 2000. Identification of the conjugated linoleic isomer that inhibits milk fat synthesis. American Journal of Physiology 278: R179–R184.Google ScholarPubMed
Beam, T. M., Jenkins, T.C., Moate, P.J., Kohn, R.A. and Palmquist, D.L. 2000. Effects of amount and source of fat on the rates of lipolysis and biohydrogenation of fatty acids in ruminal contents. Journal of Dairy Science 83: 2564–2573.Google Scholar
Cant, J. P., Fredeen, A. H., MacIntyre, T., Gunn, J. and Crowe, N. 1997. Effect of fish oil and monesin on milk composition in dairy cows. Canadian Journal of Animal Science 77: 125–131.Google Scholar
Chilliard, Y., Ferlay, A. and Doreau, M. 2001. Effect of different types of forages, animal fat or marine oils in cow’s diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livestock Production Science 70: 31–48.CrossRefGoogle Scholar
Chin, S. F. W., Liu, J.M., Storkson, Y., Ha, L. and Pariza, M.W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. Journal of Food Composition and Analysis 5: 185–197.Google Scholar
Christie, W. W. 1982. A simple procedure for rapid trans-methylation of glycerolipids and cholesteryl esters. Journal of Lipid Research 23: 1072–1075.Google Scholar
Christie, W. W., Sébédio, J. L. and Juanéda, P. 2001. A practical guide to the analysis of conjugated linoleic acid (CLA). Inform 12: 147–152.Google Scholar
Corl, B. A., Baumgard, L.H., Dwyer, D.A., Griinari, J.M., Phillipis, B.S. and Bauman, D.E. 2001. The role of delta(9)-desaturase in the production of cis-9, trans-11 CLA. Journal of Nutritional Biochemistry 12: 622–630.Google Scholar
Corl, B. A., Baumgard, L.H., Griinari, J.M., Demonte, P., Morehouse, K. M., Yurawecz, M. P. and Bauman, D.E. 2002. Trans-7, cis-9 CLA is synthesized endogenously bydelta9 desaturase in dairy cows. Lipids 37: 681–688.Google Scholar
Doreau, M. and Chilliard, Y. 1997. Effects of ruminal or post-ruminal fish oil supplementation on intake and digestion in dairy cows. Reproduction, Nutrition, Development 37: 113–124.CrossRefGoogle ScholarPubMed
Doreau, M. and Ferlay, A. 1994. Digestion and utilisation of fatty acids by ruminants. Animal Feed Science and Technology 45: 379–396.Google Scholar
France, J. and Siddons, R.C. 1986. Determination of digesta flow by continuous marker infusion. Journal of Theoretical Biology 121: 105–119.CrossRefGoogle Scholar
Fritsche, J., Yurawecz, M.P., Pawlosky, R., Flanagan, V. P., Steinhart, H. and Ku, Y. 2001. Spectroscopic characterization of unusual conjugated linoleic acid (CLA) isomers. Journal of Separation Science 24: 559–618.Google Scholar
Griinari, J. M. and Bauman, D.E. 1999. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In Advances in conjugated linoleic acid research, volume 1 (ed. Yurawecz, M. P., Mossoba, M. M., Kramer, J.K.G., Pariza, M. W. and Nelson, G. J.), pp. 180–200. AOCS Press, Champaign, IL.Google Scholar
Griinari, J. M., Corl, B.A., Lacy, S.H., Chouinard, P.Y., K.V.V., Nurmela and Bauman, D.E. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta-9-desaturase. Journal of Nutrition 130: 2285–2291.CrossRefGoogle ScholarPubMed
Griinari, J. M., Dwyer, D.A., McGuire, M.A., Bauman, D.E., Palmquist, D.L. and K.V.V., Nurmela 1998. Trans-octadecenoic acids and milk fat depression in lactating dairy cows. Journal of Dairy Science 81: 1251–1261.Google Scholar
Griinari, M. and Shingfield, K.J. 2002. Effect of diet on trans fatty acids and conjugated dienes of linoleic acid in bovine milk fat. Proceedings of the 93rd annual meeting of the American Oil Chemists Society, Montreal, Canada, S2 (abstr. ). AOCS Press, Champaign, IL.Google Scholar
Gulati, S. K., Ashes, J.R. and Scott, T.W. 1999. Hydrogenation of eicosapentaenoic and docosahexaenoic acids and their incorporation into milk fat. Animal Feed Science and Technology 79: 57–64.Google Scholar
Gulati, S. K., McHrath, M., P. C., Wynn and Scott, T.W. 2003. Preliminary results on the relative incorporation ofdocosahexaenoic and eicosapentaenoic acids into cows milkfrom two types of rumen protected fish oil. InternationalDairy Journal 13: 339–343.Google Scholar
Harfoot, C. G. and Hazelwood, G.P. 1988. Lipid metabolism in the rumen. In The rumen microbial ecosystem (ed. Hobson, P. N.), pp. 285–322. Elsevier Science, London.Google Scholar
Hashimoto, N., T., Aoyama and T., Shioiri 1981. New methods and reagents in organic synthesis. 14. A simple efficient preparation of methyl esters with trimethylsilyldiazomethane and its application to Gas Chromatographic analysis of fatty acids. Chemical and Pharmaceutical Bulletin 29: 1475–1478.Google Scholar
Huhtanen, P., Brotz, P.G. and Satter, L.D. 1997. Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cows. Journal of Animal Science 75: 1380–1392.CrossRefGoogle ScholarPubMed
Huhtanen, P. and Kukkonen, U. 1995. Comparison of methods, markers, sampling sites and models for estimating digesta passage kinetics in cattle fed at two levels of intake. Animal Feed Science and Technology 52: 141–158.Google Scholar
Jiang, J., A., Wolk and B., Vessby 1999. Relation between the intake of milk fat and the occurence of conjugated linoleic acid in human adipose tissue. American Journal of Clinical Nutrition 70: 21–27.CrossRefGoogle ScholarPubMed
Keady, T. W. J. and Mayne, C.S. 1999. The effects of level of fish oil inclusion in the diet on rumen digestion and fermentation parameters in cattle offered grass silage based diets. Animal Feed Science and Technology 81: 57–68.Google Scholar
Keady, T. W. J., Mayne, C.S. and Fitzpatrick, D.A. 2000. Effects of supplementation of dairy cattle with fish oil on silage intake, milk yield and milk composition. Journal of Dairy Research 67: 137–153.CrossRefGoogle ScholarPubMed
Kitessa, S. M., Gulati, S.K., Ashes, J.R., Fleck, E., Scott, T. W. and Nichols, P.D. 2001. Utilisation of fish oil in ruminants. II. Transfer of fish into goats’ milk. Animal Feed Scence and Technology 89: 201–208.Google Scholar
Kramer, J. K. G., Fellner, V., M.E.R., Dugan, Sauer, F. D., M. M., Mossoba and Yurawecz, M.P. 1997. Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32: 1219–1228.CrossRefGoogle ScholarPubMed
Kritchevsky, D. 2000. Antimutagenic and some other effects of conjugated linoleic acid. British Journal of Nutrition 83: 459–465.CrossRefGoogle ScholarPubMed
Mosley, E., Powell, G.L., Riley, M.B. and Jenkins, T.C. 2002. Microbial biohydrogenation of oleic acid to trans isomers in vitro . Journal of Lipid Research 43: 290–296.CrossRefGoogle ScholarPubMed
Noble, R. C. 1981. Digestion, transport and absorption of lipids. In Lipid metabolism in ruminant animals (ed. Christie, W. W.), pp. 57–93. Pergamon Press Ltd, Oxford.CrossRefGoogle Scholar
Offer, N. W., Marsden, M., Dixon, J., B. K., Speake and Thacker, F.E. 1999. Effect of dietary fat supplements on levels of n-3 poly-unsaturated fatty acids, trans acids and conjugated linoleic acid in bovine milk. Animal Science 69: 613–625.Google Scholar
Offer, N. W., Speake, B.K., Dixon, J. and M., Marsden 2001. Effect of fish-oil supplementation on levels of (n-3) poly-unsaturated fatty acids in the lipoprotein fractions of bovine plasma. Animal Science 73: 523–531.Google Scholar
Ogawa, J., Matsumaura, K., Kishino, S., Omura, Y. and S., Shimizu 2001. Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Applied and Environmental Microbiology 67: 1246–1252.CrossRefGoogle ScholarPubMed
Park, Y., Albright, K.J., Cai, Z.Y. and Pariza, M.W. 2001. Comparison of methylation procedures for conjugated linoleic acid and artifact formation by commercial (trimethylsilyl)diazomethane. Journal of Agricultural and Food Chemistry 49: 1158–1164.Google Scholar
Parodi, P. W. 1999. Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. Journal of Dairy Science 82: 1339–1349.CrossRefGoogle ScholarPubMed
Parodi, P. W. 1977. Conjugated octadeceadienoic acids of milk fat. Journal of Dairy Science 60: 1550–1553.CrossRefGoogle Scholar
Pennington, J. A. and Davis, C.L. 1975 Effects of intraruminal and intra-abomasal additions of cod liver oil on milk fat production in the cow. Journal of Dairy Science 58: 49–55.CrossRefGoogle ScholarPubMed
Piperova, L. S., Sampugna, L., Teter, B. B., Kalscheur, K. F., Yurawecz, M. P., Ku, Y., Morehouse, K. M. and Erdman, R.A. 2002. Duodenal and milk trans octadecanoic acid and conjugated linoleic acid (CLA) isomers indicate that postabsorptive synthesis is the predominant source of cis-9-containing CLA in lactating dairy cows. Journal of Nutrition 132: 1235–1241.CrossRefGoogle Scholar
Polan, C. E., McNeill, J.J. and Tove, S.B. 1964. Biohydrogenation of unsaturated fatty acids by rumen bacteria. Journal of Bacteriology 88: 1056–1064.Google Scholar
Precht, D. and Molkentin, J. 1997. Trans-geometrical and positional isomers of linoleic acid including conjugated linoleic acid (CLA) in German milk and vegetable fats. Lipid 99: 319–326.Google Scholar
Precht, D., Molkentin, J., McGuire, M. A., McGuire, M. K. and Jensen, R.G. 2001. Overestimates of oleic and linoleic acid contents in materials containing trans fatty acids and analyzed with short packed gas chromatographic columns. Lipids 36: 213–216.Google Scholar
Radin, N. S. 1981. Extraction of tissue lipids with a solvent of low toxicity. Methods in Enzymology 72: 5–8.Google Scholar
Roche, H. M., Noone, E., Nugent, A. and Gibney, M.J. 2001. Conjugated linoleic acid: a novel therapeutic nutrient? Nutrition Research Reviews 14: 173–187.Google Scholar
Scollan, N. D., Dhanoa, M.S., Choi, N.J., Maeng, W.J., Enser, M. and Wood, J.D. 2001. Biohydrogenation and digestion of long chain fatty acids in steers fed on different sources of lipid. Journal of Agricultural Science, Cambridge 136: 345–355.Google Scholar
Sehat, N., Yurawecz, M.P., J.A.G., Roach, Mossoba, M. M., J.K.G., Kramer and Ku, Y. 1998. Silver-ion high performance liquid chromatographic separation and identification of conjugated linoleic acid isomers. Lipids 33: 217–221.Google Scholar
Shingfield, K. J., Jaakkola, S. and Huhtanen, P. 2001. Effects of level of nitrogen fertilizer application and various nitrogenous supplements on milk production and nitrogen utilization of dairy cows given grass silage-based diets. Animal Science 73: 541–554.CrossRefGoogle Scholar
Shingfield, K. J., Jaakkola, S. and Huhtanen, P. 2002. Effect of forage conservation method, concentrate level and propylene glycol on diet digestibility, rumen fermentation, blood metabolite concentrations and nutrient utilization of dairy cows. Animal Feed Science and Technology 97: 1–21.Google Scholar
Sjaunja, L. O., Baeve, L., Junkkarinen, L., Pedersen, J. and Setälä, J. 1990. A Nordic proposal for an energy corrected milk (ECM) formula. Twenty-seventh session of the International Committee of Recording and Productivity of Milk Animals, Paris, pp. 156–157.Google Scholar
Statistical Analysis Systems Institute. 1989. SAS/STAT®user´s guide, version 6, fourth edition. SAS Institute Inc., Cary, NC. Google Scholar
Sukhija, P. S. and Palmquist, D.L. 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36: 1202–1206.Google Scholar
Varman, P. N., Schultz, L.H. and Nichols, R.E. 1968. Effect of unsaturated oils on rumen fermentation, blood components and milk composition. Journal of Dairy Science 51: 1956–1963.Google Scholar
Wachira, A. M., Sinclair, L.A., Wilkinson, R.G., Hallett, K., M., Enser and Wood, J.D. 2000. Rumen biohydrogenation of n-3 polyunsaturated fatty acids and their effects on microbial efficiency and nutrient digestibility in sheep. Journal of Agricultural Science, Cambridge 135: 419–428.Google Scholar
Williams, C. M. 2000. Dietary fatty acids and human health. Annales de Zootechnie 49: 165–180.Google Scholar
Wonsil, B. J., Herbein, J.H. and Watkins, B.A. 1994. Dietary and ruminally derived trans-18: 1 fatty acids alter bovine milk lipids. Journal of Nutrition 124: 556–565.Google Scholar
Yurawecz, M. P., J.K.G., Kramer and Ku, Y. 1999. Methylation procedures for conjugated linoleic acid. In Advances in conjugated linoleic acid research, volume 1 (ed. Yurawecz, M. P., Mossoba,, M. M. Kramer, J.K.G., Pariza, M. W. and Nelson, G. J.), pp. 64–82. AOCS Press, Champaign, IL.Google Scholar