Hostname: page-component-848d4c4894-x5gtn Total loading time: 0 Render date: 2024-05-18T22:23:48.563Z Has data issue: false hasContentIssue false
  • English
  • Français

Association between gene polymorphism of growth hormone and carcass traits in dairy bulls

Published online by Cambridge University Press:  18 August 2016

R. Grochowska ,
A. Lundén ,
L. Zwierzchowski ,
M. Snochowski  and
J. Oprządek
Get access

Abstract

The contribution of the leucine/valine substitution at amino acid position 127 in the bovine growth hormone (GH) protein to variation in carcass traits was studied. The data included 109 Polish Friesian bulls slaughtered at 15 months of age. The traits measured were carcass gain, weights of meat, bones, intermuscular and subcutaneous fat in the carcass and meat, bones and fat in valuable cuts (fore and best ribs, sirloin, round of beef and shoulder). The bulls’ GH genotype was determined using the PCR-RFLP technique. The frequencies of leucine (Leu) and valine (Val) alleles were 0·64 and 0·36, respectively. The GH concentration was determined in serial blood plasma samples collected every 15 min starting from 15 min before to 135 min after intravenous administration of 0·15 µg thyrotropin releasing hormone (TRH) per kg live weight. Response GH variables were: baseline (the mean of samples collected at –15 and 0 min), peak (the sample taken at 15 min post injection of TRH) and disappearance rate (calculated as peak minus the sample at 60 min, divided by time interval 45 min). Mixed animal models were used for the statistical analysis. Differences were found between the Leu/Leu and the Val/Val genotypes for carcass gain and weight of meat in the carcass (P ≤ 0·05). Moreover, differences in the size of the GH peak between the two homozygotes approached significance (P ≤ 0·10). The effect of GH genotype accounted for a moderate part of the phenotypic variance in the carcass traits, corresponding to a reduction in the residual variance of ≤ 5·25% when included in the model, whereas the corresponding value for the effect of GH genotype on the variation in GH release was lower, ≤ 1·77%. In conclusion, the Leu/Val polymorphism seems to be associated with carcass traits in dairy bulls, although the effect was relatively small when compared with the effects of season and background genome.

Keywords

carcass compositiondairy bullsgene polymorphismsomatotropin
Type
Breeding and genetics
Information
Animal Science , Volume 72 , Issue 3 , June 2001 , pp. 441 - 447
Copyright
Copyright © British Society of Animal Science 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdel-Meguid, S.S, Shieh, H. S., Smith, W. W., Dayringer, H. E., Violand, B. N. and Bentle, L. A. 1987. Three-dimensional structure of a genetically engineered variant of porcine growth hormone. Proceedings of the National Academy of Sciences of the United States of America 84: 6434–6437.Google Scholar
Chen, W. Y., Wight, D. C., Chen, N. Y., Coleman, T. A., Wagner, T. E. and Kopchick, J. J. 1991. Mutations in the third α-helix of bovine growth hormone dramatically affect its intracellular distibution in vitro and growth enhacement in transgenic mice. Journal of Biological Chemistry 266: 2252–2258.Google Scholar
Chrząszcz, T. and Janicki, M. A. 1962. [Methods for carcass estimation in cattle.] Manuscript. ZD Koluda Wielka.Google Scholar
De Vos, A. M., Ultsch, M. and Kossiakoff, A. A. 1992. Human growth hormone and extracellular domain of its receptor: crystal structure of the complex. Science 225: 306–312.CrossRefGoogle Scholar
Dvorak, P., Becka, S., Krejci, P. and Chrpova, M. 1978. Radioimmunoassay of bovine growth hormone. Biochemical Radioanalitical Letters 34: 155–160.Google Scholar
Falconer, D. S. and Mackay, T. F. C. 1996. Introduction to quantitative genetics, fourth edition. Longman Group Ltd, Essex, England.Google Scholar
Gluckman, P. D., Breier, B. H. and Davis, S. R. 1987. Physiology of the somatotropic axis with particular reference to the ruminant. Journal of Dairy Science 70: 442–466.Google Scholar
Grochowska, R., Zwierzchowski, L., Snochowski, M. and Reklewski, Z. 1999. Stimulated growth hormone (GH) release in Friesian cattle with respect to GH genotypes. Reproduction, Nutrition, Development 39: 171–180.Google Scholar
Groeneveld, E. M., Kovac, M. and Wang, T. 1990. PEST. A general purpose BLUP package for multivariate prediction and estimation. Proceedings of the fourth world congress on genetics applied to livestock production, Edinburgh, Scotland, vol. 12, p. 488.Google Scholar
Kawasaki, E. S. 1990. Sample preparation from blood, cells and others fluids. In PCR protocols: a guide to methods and application (ed. Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J.), pp. 3-12, Academic Press, New York.Google Scholar
Lee, B. K., Lin, G. F., Crooker, B. A., Murtaugh, M. P., Hansen, L. B. and Chester-Jones, H. 1996. Association of somatotropin (bST) gene polymorphism at the 5th exon with selection for milk yield in Holstein cows. Domestic Animal Endocrinology 13: 373–381.Google Scholar
Løvendahl, P. and Sejrsen, K. 1993. Endogenous growth hormone release in calves selected for milk fat yield. Animal Production 56: 285–291.Google Scholar
Lucy, M. C., Hauser, S.D., Eppard, P. J., Krivi, G. G., Clark, J. H., Bauman, D. E. and Collier, R. J. 1993. Variants of somatotropin in cattle: gene frequencies in major dairy breeds and associated milk production. Domestic Animal Endocrinology 10: 325–333.Google Scholar
Lucy, M. C., Hauser, S.D., Eppard, P. J., Krivi, G. G. and Collier, R. J. 1991. Genetic polymorhism within the bovine somatotropin (bST) gene detected by polymerase chain reaction and endonuclease digestion. Journal of Dairy Science 74: (suppl. 1) 284 (abstr. ).Google Scholar
Neathery, M. W., Crowe, C. T., Hartnell, G. F., Veenhuizen, J. J., Reagan, J. O. and Blackmon, D. M. 1991. Effects of sometribove on performance, carcass composition and chemical blood characteristics of dairy calves. Journal of Dairy Science 74: 3933–3939.Google Scholar
Oprządek, J., Dymnicki, E., Zwierzchowski, L. and Łukaszewicz, M. 1999. The effect of growth hormone (GH), κ-kasein (CASK) and β-lactoglobulin (BLG) genotypes on carcass traits in Friesian bulls. Animal Science Papers and Reports — Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzebiec 17: 85–92.Google Scholar
Polish Norms. 1993. Nutrient requirements of ruminants. Institute of Zootechnics, Omnitech Press, Warsaw.Google Scholar
Schlee, P., Graml, R., Rottmann, O. and Pirchner, F. 1994a. Influence of growth hormone genotypes on breeding values of Simmental bulls. Journal of Animal Breeding and Genetics 111: 253–256.Google Scholar
Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottmann, O., Olbrich-Bludau, A. and Pirchner, F. 1994b. Growth hormone and insulin-like growth factor I concentrations in bulls of various growth hormone genotypes. Theoretical and Applied Genetics 88: 497–500.Google Scholar
Statistical Analysis Systems Institute. 1989. SAS/STAT user’s guide, fourth edition, version 6, vol. 2. SAS Institute Inc., Cary, NC, USA.Google Scholar
Werf, J. H. J. van der, Verburg, F. and Garssen, G. J. 1996. Evidence for a strong effect of the AluI polymorphism in the growth hormone gene on yield characteristics in dairy cattle. Proceedings of the 47th meeting of the European Association for Animal Production, 25-29 August, Lillehammer, Norway, abstract no 1, p. 310.Google Scholar
Zwierzchowski, L., Żelazowska, B. and Grochowska, R. 1995. Genotyping of kappa-casein and growth hormone alleles in Polish Black and White and Piemontese cattle using PCR-RFLP technique. Animal Science Papers and Reports — Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzebiec 13: 13–20.Google Scholar