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  • Print publication year: 2021
  • Online publication date: March 2021

Chapter 12 - Cryopreservation

from Section 2 - Assisted Reproductive Procedures


Cryopreservation involves the cooling of cells and tissues to subzero temperatures to stop all biological activity and preserve them for future use. Initial cryopreservation methods were ineffective as simple cooling techniques led to cellular damage due to the altered concentration of solutes within the cells, ice formation, and excessive dehydration.

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