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Chapter 14 - Technical Considerations in Perinatal CNS Tissue Processing

from Tissue Selection

Published online by Cambridge University Press:  07 August 2021

Mirna Lechpammer ,
Marc Del Bigio  and
Rebecca Folkerth
By
Mirna Lechpammer
Mirna Lechpammer
Affiliation:
New York University School of Medicine
Marc Del Bigio
Affiliation:
University of Manitoba, Canada
Rebecca Folkerth
Affiliation:
New York University School of Medicine
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Summary

Traditionally, autopsy comprises of gross and microscopic examination, which routinely involves tissue fixation, processing, and paraffin embedding. Perinatal autopsies present with a variety of challenges, not the least of which involves the removal and examination of the immature brain. Even if great care is taken during brain removal, one may be left with an amorphous, semifluid mass of softened tissue by the time the brain is ready for fixation and further processing [1]. Therefore, in Appendix 1, we summarize the preparation of recommended fixative, manual, and automated procedures for the processing of perinatal brain tissue, which in our experience help to preserve CNS morphology and allow further downstream tissue studies.

Type
Chapter
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Perinatal Neuropathology , pp. 62 - 64
Publisher: Cambridge University Press
Print publication year: 2021

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References

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