Skip to main content Accessibility help
×
Hostname: page-component-7c8c6479df-hgkh8 Total loading time: 0 Render date: 2024-03-28T00:37:17.199Z Has data issue: false hasContentIssue false

Chapter 5 - Morphological Sperm Selection Before ICSI

Published online by Cambridge University Press:  02 December 2021

Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
Get access

Summary

Application of intracytoplasmic morphologically selected sperm injection (IMSI) is a matter of debate; however, it is evident that deselection of spermatozoa presenting respectively spermatozoa with vacuole-like structures and asymmetrical insertion of the neck has multiple benefits. Defective sperm function and molecular defects contribute to early embryonic development disturbance, failure of blastocyst formation, miscarriages, and congenital birth defects. Introduction of IMSI has made embryologists aware that for ICSI the selection of sperm should be given proper attention. We have to consider IMSI has improved the ICSI technique. Compared to “blind” spermatozoa aspiration at low magnification and/or reduced optics contrast, observation under optimal contrast optics increases the probability of selecting a normal spermatozoa. This is particularly evident in cases of severe teratozoospermia, with low responder and advanced age women. Practicable and technical aspects of morphologically normal sperm selection must be considered to facilitate the workflow while performing IMSI and avoiding damage to the oocytes. A proper set up of the dish for selecting and capturing sperm facilitates routine application of IMSI.

Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
, pp. 49 - 58
Publisher: Cambridge University Press
Print publication year: 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Nikolettos, N, Kiipker, W, Demirel, C, Schopper, B, Blasig, C, Sturm, R, et al. Fertilization potential of spermatozoa with abnormal morphology. Hum Reprod. 1999; 14 (Suppl. 1), 4770.Google Scholar
Palermo, G, Joris, H, Devroey, P, Van Steirteghem, A. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992; 340, 1718.Google Scholar
Schagdarsurengin, U, Paradowska, A, Steger, K. Analysing the sperm epigenome: roles in early embryogenesis and assisted reproduction. Nat Rev Urol. 2012; 9, 609–19.CrossRefGoogle ScholarPubMed
Cassuto, NG, Hazout, A, Bouret, D, Balet, R, Larue, L, Benifla, JL, et al. Low birth defects by deselecting abnormal spermatozoa before ICSI. Reprod Biomed Online. 2014; 28, 4753.CrossRefGoogle ScholarPubMed
Bartoov, B, Berkovitz, A, Eltes, F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001; 345, 1067–8.Google Scholar
Boitrelle, F, Ferfouri, F, Petit, JM, Segretain, D, Tourain, C, Bergere, M, et al. Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod. 2011; 26, 1650–8.Google Scholar
Cassuto, NG, Hazout, A, Hammoud, I, Balet, R, Bouret, D, Barak, Y, et al. Correlation between DNA defect and sperm-head morphology. Reprod Biomed Online. 2012; 24, 211–18.Google Scholar
Vanderzwalmen, P, Imbert, R, Jareno-Martinez, D, Stecher, A, Vansteenbrugge, A, Vanderzwalmen, S, et al. Intracytoplasmic morphologically selected sperm injection. Nagy, ZP et al., (eds), In Vitro Fertilization: A Textbook of Current and Emerging Methods and Devices (Second ed.) Springer Nature, Switzerland 2019; 415428.CrossRefGoogle Scholar
Zheng, H, Stratton, C, Morozumi, K, Jin, J, Yanagimachi, R, Yan, W. Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility. PNAS. 2007; 104:6852–7.CrossRefGoogle ScholarPubMed
Gaspard, O, Vanderzwalmen, P, Wirleitner, B, Ravet, S, Wenders, F, Eichel, V, et al. Impact of high magnification sperm selection on neonatal outcomes: a retrospective study. J Assist Reprod Genet. 2018; 35: 1113–21.Google Scholar
Tavalaee, M, Razavi, S, Nasr-Esfahani, MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril. 2009; 91, 1119–26.Google Scholar
Oliva, R, Ballesca, JL. Altered histone retention and epigenetic modifications in the sperm of infertile men. Asian J Androl. 2012; 14, 239240.Google Scholar
Franco, JG Jr, Mauri, AL, Petersen, CG, Massaro, FC, Silva, LF, Felipe, V, et al. Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl. 2012; 35, 4651.Google Scholar
Boitrelle, F, Albert, M, Petit, J-M, Ferfouri, F, Wainer, R, Bergere, M, et al. Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure. Reprod Biomed Online. 2013; 27, 201–11.CrossRefGoogle ScholarPubMed
Shaoqin, G, Zhenghui, Z, Xueqian, Z, Yuan, H. [Epigenetic modifications in human spermatozoon and its potential role in embryonic development.] Yi Chuan. 2014; 36, 439–46.Google Scholar
Schagdarsurengin, U., Paradowska, A., Steger, K. Analysing the sperm epigenome: roles in early embryogenesis and assisted reproduction. Nat Rev Urol. 2012; 9, 609–19.Google Scholar
Marques, CJ, Carvalho, F, Sousa, M, Barros, A. Genomic imprinting in disruptive spermatogenesis. Lancet. 2004; 363, 1700–2.Google Scholar
Cassuto, G, Montjean, D, Siffroi, JP, Bouret, D, Marzouk, F, Copin, H, Benkhalifa, M. Different levels of DNA methylation detected in human sperms after morphological selection using high magnification microscopy. BioMed Res Int. 2016; 17 http://dx.doi.org/10.1155/2016/6372171.CrossRefGoogle Scholar
Van Blerkom, J, Davis, P. Evolution of the sperm aster after microinjection of isolated human sperm centrosomes into meiotically mature human oocytes. Hum Reprod. 1995; 10, 2179–82.CrossRefGoogle ScholarPubMed
Ugajin, T, Terada, Y, Hasgawa, H, Nabeshima, H, Suzuki, K, Yaegashi, N. The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function. J Assist Reprod Genet. 2010; 27, 7581.CrossRefGoogle ScholarPubMed
Cassuto, NG, Bouret, D, Plouchart, JM, Jellad, S, Vanderzwalmen, P, Balet, R, et al. A new real-time morphology classification for human spermatozoa : a link for fertilization and improved embryo quality. Fertil Steril. 2009; 92, 1616–25.CrossRefGoogle ScholarPubMed
Leandri, RD, Gachet, A, Pfeffer, J, Celebi, C, Rives, N, Carre–Pigeon, F, et al. Is intracytoplasmic morphologically selected sperm injection (IMSI) beneficial in the first ART cycle? A multicentric randomized controlled trial. Andrology. 2013; 1, 692–7.Google Scholar
Knez, K, Zorn, B, Tomazevic, T, Vrtacnik–Bokal, E, Virant–Klun, I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: a comparative prospective randomized study. Reprod Biol Endocrinol. 2011; 9, 123.CrossRefGoogle ScholarPubMed
Balaban, B, Yakin, K, Alatas, C, Oktem, O, Isiklar, A, Urman, B. Clinical outcome of intracytoplasmic injection of spermatozoa morphologically selected under high magnification: a prospective randomized study. Reprod Biomed Online. 2011; 22, 472–6.Google Scholar
Setti, AS, Braga, DP, Figueira, RC, Iaconelli, A, Jr., Borges, E. Intracytoplasmic morphologically selected sperm injection results in improved clinical outcomes in couples with previous ICSI failures or male factor infertility: a meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2014; 183, 96103.Google Scholar
Shalom-Paz, E, Anabusi, S, Michaeli, M, Karchovsky-Shoshan, E, Rothfarb, N, Shavit, T, Ellenbogen, A. Can intra cytoplasmatic morphologically selected sperm injection (IMSI) technique improve outcome in patients with repeated IVF-ICSI failure? A comparative study. Gynecol Endocrinol. 2015; 31, 247–51.Google Scholar
Vanderzwalmen, P, Hiemer, A, Rubner, P, Bach, M, Neyer, A, Astrid Stecher, A, et al. Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online. 2008; 17, 617–27.CrossRefGoogle ScholarPubMed
Knez, K, Zorn, B, Tomazevic, T, Vrtacnik–Bokal, E, Virant-Klun, I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: A comparative prospective randomized study Reprod Biol Endocrinol. 2011; 9, 123–30.Google Scholar
Cassuto, NG, Hazout, A, Bouret, D, Balet, R, Larue, L, Benifla, JL, Viot, G. Low birth defects by deselecting abnormal spermatozoa before ICSI. Reprod Biomed Online. 2014; 28, 4753.Google Scholar
Vanderzwalmen, S, Jareno, D, Vanderzwalmen, P, Imber, R, Murtinger, M, Wirleitner, B. Sperm selection for ICSI by morphology. Montag, M. Morbeck, D (Eds.) Principles of IVF Laboratory Practice: Optimizing Performance and Outcomes. Cambridge University Press 2017, 163171.Google Scholar
Van der Zwalmen, P, Bertin-Segal, G, Geerts, L, Debauche, C, Schoysman, R. Sperm morphology and IVF pregnancy rate: comparison between Percoll gradient centrifugation and swim-up procedures. Hum Reprod. 1991; 4, 581–8.Google Scholar
Berkovitz, A, Dekel, Y, Goldstein, R, Bsoul, S, Machluf, Y, Bercovich, D. The significance of human spermatozoa vacuoles can be elucidated by a novel procedure of array comparative genomic hybridization. Hum Reprod. 2018; 33, 563–71.Google Scholar
Peer, S, Eltes, F, Berkovitz, A, Yehuda, R, Itsykson, P, Bartoov, B. Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 C? Fertil Steril. 2007; 88, 1589–94.Google Scholar
Neyer, A, Vanderzwalmen, P, Bach, M, Stecher, A, Spitzer, D, Zech, N. Sperm head vacuoles are not affected by in-vitro conditions, as analysed by a system of sperm-microcapture channels. Reprod Biomed Online. 2013; 26, 368–77.Google Scholar
Denomme, MM, McCallie, BR, Parks, JC, Schoolcraft, WB, Katz–Jaffe, MG. Alterations in the sperm histone-retained epigenome are associated with unexplained male factor infertility and poor blastocyst development in donor oocyte IVF cycles. Hum Reprod. 2017; 32, 2443–55.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×