Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-25T15:14:45.782Z Has data issue: false hasContentIssue false

25 - Omics as tools for oocyte selection

from Section 5 - Pathology

Published online by Cambridge University Press:  05 October 2013

By
Marc-André Sirard  and
Isabelle Gilbert
Edited by
Alan Trounson ,
Roger Gosden  and
Ursula Eichenlaub-Ritter
Marc-André Sirard
Affiliation:
Université Laval, Quebec City, Quebec, Canada
Isabelle Gilbert
Affiliation:
Université Laval, Quebec City, Quebec, Canada
Alan Trounson
Affiliation:
California Institute for Regenerative Medicine
Roger Gosden
Affiliation:
Center for Reproductive Medicine and Infertility, Cornell University, New York
Ursula Eichenlaub-Ritter
Affiliation:
Universität Bielefeld, Germany
Get access

Summary

Introduction

The neologism “omics” encompasses several new disciplines, such as genomics, transcriptomics, proteomics, metabolomics, and epigenomics, associated with the molecular analysis of biological events (Figure 25.1). Recently, a combination of whole genome sequencing; to RNA sequencing; and to proteomic, metabolomic, and autoantibody profiling has been realized from an individual to obtain an “integrative personal omics profile” (iPOP) [1]. This individual profile offers the perspective of a personal medicine and predicts future health and disease possibilities. For fertility comprehension, omics offers all the tools required for an in-depth study of the different steps required for a successful reproductive process. Since about 15% of couples have fertility problems, omics offers also diagnostic possibilities for assisted reproduction. For example, follicular cells (as well as cumulus cells) transcriptomes can help to predict which oocyte has the best potential to develop into an embryo or to determine the embryo(s) most likely to result in a pregnancy.

Omics include all high-throughput techniques of every cellular metabolic component. At the DNA level, genomics refers to whole genome sequencing, DNA polymorphisms (such as single-nucleotide polymorphism [SNP]) or sequence variation (such as insertion, deletion, duplication, and copy number variants), etc. For RNA, transcriptomics studies the transcribed genome including mainly messenger RNA (mRNA) but also the ribosomal RNA (rRNA), transfer RNA (tRNA), and other non-coding RNA. When mRNAs have been translated, proteomics studies proteins, especially their structures and functions. Metabolomics involves the study of cellular activities with small-molecule metabolites profiles. Finally, epigenetics refers to the comprehension of different events (such as DNA methylation and histone modification) which influence gene expression without altering the DNA sequence. Taken together, integration of all these omics fields in a systems biology perspective is the ultimate research goal in order to give the complete picture of a cell. However, achieving this concept remains a big challenge.

Type
Chapter
Information
Biology and Pathology of the Oocyte
Role in Fertility, Medicine and Nuclear Reprograming
 , pp. 297 - 305
Publisher: Cambridge University Press
Print publication year: 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chen, R, Mias, GI, Li-Pook-Than, J, et al. Personal omics profiling reveals dynamic molecular and medical phenotypes. Cell 2012; 148(6): 1293–307.CrossRefGoogle ScholarPubMed
Gilbert, I, Scantland, S, Sylvestre, EL, et al. Providing a stable methodological basis for comparing transcript abundance of developing embryos using microarrays. Mol Hum Reprod 2010; 16(8): 601–16.CrossRefGoogle ScholarPubMed
Lalioti, MD.Can preimplantation genetic diagnosis overcome recurrent pregnancy failure?Curr Opin Obstet Gynecol 2008; 20(3): 199–204.CrossRefGoogle ScholarPubMed
Harper, JC, Sengupta, SB.Preimplantation genetic diagnosis: state of the art 2011. Hum Genet 2012; 131(2): 175–86.CrossRefGoogle ScholarPubMed
Ly, KD, Agarwal, A, Nagy, ZP.Preimplantation genetic screening: does it help or hinder IVF treatment and what is the role of the embryo?J Assist Reprod Genet 2011; 28(9): 833–49.CrossRefGoogle ScholarPubMed
Basille, C, Frydman, R, El Aly, A, et al. Preimplantation genetic diagnosis: state of the art. Eur J Obstet Gynecol Reprod Biol 2009; 145(1): 9–13.CrossRefGoogle ScholarPubMed
Checa, MA, Alonso-Coello, P, Sola, I, et al. IVF/ICSI with or without preimplantation genetic screening for aneuploidy in couples without genetic disorders: a systematic review and meta-analysis. J Assist Reprod Genet 2009; 26(5): 273–83.CrossRefGoogle ScholarPubMed
Wells, D, Escudero, T, Levy, B, et al. First clinical application of comparative genomic hybridization and polar body testing for preimplantation genetic diagnosis of aneuploidy. Fertil Steril 2002; 78(3): 543–9.CrossRefGoogle ScholarPubMed
Dubey, AK, Wang, HA, Duffy, P, Penzias, AS.The correlation between follicular measurements, oocyte morphology, and fertilization rates in an in vitro fertilization program. Fertil Steril 1995; 64(4): 787–90.CrossRefGoogle Scholar
Nataprawira, DS, Harada, T, Sekijima, A, Mio, Y, Terakawa, N.Assessment of follicular maturity by follicular diameter and fluid volume in a program of in vitro fertilization and embryo transfer. Asia Oceania J Obstet Gynaecol 1992; 18(3): 225–30.Google Scholar
Wittmaack, FM, Kreger, DO, Blasco, L, et al. Effect of follicular size on oocyte retrieval, fertilization, cleavage, and embryo quality in in vitro fertilization cycles: a 6-year data collection. Fertil Steril 1994; 62(6): 1205–10.CrossRefGoogle ScholarPubMed
Ectors, FJ, Vanderzwalmen, P, Van Hoeck, J, et al. Relationship of human follicular diameter with oocyte fertilization and development after in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 1997; 12(9): 2002–5.CrossRefGoogle ScholarPubMed
Arnot, AM, Vandekerckhove, P, DeBono, MA, Rutherford, AJ.Follicular volume and number during in-vitro fertilization: association with oocyte developmental capacity and pregnancy rate. Hum Reprod 1995; 10(2): 256–61.CrossRefGoogle ScholarPubMed
Bergh, C, Broden, H, Lundin, K, Hamberger, L.Comparison of fertilization, cleavage and pregnancy rates of oocytes from large and small follicles. Hum Reprod 1998; 13(7): 1912–15.CrossRefGoogle ScholarPubMed
Teissier, MP, Chable, H, Paulhac, S, Aubard, Y.Comparison of follicle steroidogenesis from normal and polycystic ovaries in women undergoing IVF: relationship between steroid concentrations, follicle size, oocyte quality and fecundability. Hum Reprod 2000; 15(12): 2471–7.CrossRefGoogle ScholarPubMed
Salha, O, Nugent, D, Dada, T, et al. The relationship between follicular fluid aspirate volume and oocyte maturity in in-vitro fertilization cycles. Hum Reprod 1998; 13(7): 1901–6.CrossRefGoogle ScholarPubMed
Lee, TF, Lee, RK, Hwu, YM, et al. Relationship of follicular size to the development of intracytoplasmic sperm injection-derived human embryos. Taiwan J Obstet Gynecol 2010; 49(3): 302–5.CrossRefGoogle ScholarPubMed
Shima, K, Kitayama, S, Nakano, R.Gonadotropin binding sites in human ovarian follicles and corpora lutea during the menstrual cycle. Obstet Gynecol 1987; 69(5): 800–6.Google ScholarPubMed
Farhi, J, Orvieto, R, Homburg, R.Administration of clomiphene citrate in patients with polycystic ovary syndrome, without inducing withdrawal bleeding, achieves comparable treatment characteristics and outcome. Fertil Steril 2010; 93(6): 2077–9.CrossRefGoogle ScholarPubMed
Guzman, L, Ortega-Hrepich, C, Albuz, FK, et al. Developmental capacity of in vitro-matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm. Fertil Steril 2012; 98(2): 503-7.e1–2.CrossRefGoogle ScholarPubMed
Trounson, A, Anderiesz, C, Jones, G.Maturation of human oocytes in vitro and their developmental competence. Reproduction 2001; 121(1): 51–75.CrossRefGoogle ScholarPubMed
Andersen, CY.Characteristics of human follicular fluid associated with successful conception after in vitro fertilization. J Clin Endocrinol Metab 1993; 77(5): 1227–34.Google ScholarPubMed
Artini, PG, Battaglia, C, D’Ambrogio, G, et al. Relationship between human oocyte maturity, fertilization and follicular fluid growth factors. Hum Reprod 1994; 9(5): 902–6.CrossRefGoogle ScholarPubMed
Greenseid, K, Jindal, S, Hurwitz, J, Santoro, N, Pal, L.Differential granulosa cell gene expression in young women with diminished ovarian reserve. Reprod Sci 2011; 18(9): 892–9.CrossRefGoogle ScholarPubMed
Chian, RC, Buckett, WM, Too, LL, Tan, SL.Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin. Fertil Steril 1999; 72(4): 639–42.CrossRefGoogle ScholarPubMed
Sirard, MA.Follicle environment and quality of in vitro matured oocytes. J Assist Reprod Genet 2011; 28(6): 483–8.CrossRefGoogle ScholarPubMed
Chian, RC, Buckett, WM, Tulandi, T, Tan, SL.Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000; 15(1): 165–70.CrossRefGoogle ScholarPubMed
Gelbaya, TA, Tsoumpou, I, Nardo, LG.The likelihood of live birth and multiple birth after single versus double embryo transfer at the cleavage stage: a systematic review and meta-analysis. Fertil Steril 2009; 94(3): 936–45.CrossRefGoogle ScholarPubMed
Felberbaum, RE.Multiple pregnancies after assisted reproduction – international comparison. Reprod Biomed Online 2007; 15 Suppl 3: 53–60.CrossRefGoogle ScholarPubMed
Bedard, J, Brule, S, Price, CA, Silversides, DW, Lussier, JG.Serine protease inhibitor-E2 (SERPINE2) is differentially expressed in granulosa cells of dominant follicle in cattle. Mol Reprod Dev 2003; 64(2): 152–65.CrossRefGoogle ScholarPubMed
Evans, AC, Ireland, JL, Winn, ME, et al. Identification of genes involved in apoptosis and dominant follicle development during follicular waves in cattle. Biol Reprod 2004; 70(5): 1475–84.CrossRefGoogle ScholarPubMed
Mihm, M, Baker, PJ, Ireland, JL, et al. Molecular evidence that growth of dominant follicles involves a reduction in follicle-stimulating hormone dependence and an increase in luteinizing hormone dependence in cattle. Biol Reprod 2006; 74(6): 1051–9.CrossRefGoogle ScholarPubMed
Wells, D, Patrizio, P.Gene expression profiling of human oocytes at different maturational stages and after in vitro maturation. Am J Obstet Gynecol 2008; 198(4): 455.e1–9; discussion 455.e9–11.CrossRefGoogle ScholarPubMed
Gilbert, I, Robert, C, Dieleman, S, Blondin, P, Sirard, MA.Transcriptional effect of the LH surge in bovine granulosa cells during the peri-ovulation period. Reproduction 2011; 141(2): 193–205.CrossRefGoogle ScholarPubMed
Grondahl, ML, Borup, R, Lee, YB, et al. Differences in gene expression of granulosa cells from women undergoing controlled ovarian hyperstimulation with either recombinant follicle-stimulating hormone or highly purified human menopausal gonadotropin. Fertil Steril 2009; 91(5): 1820–30.CrossRefGoogle ScholarPubMed
Bettegowda, A, Patel, OV, Lee, KB, et al. Identification of novel bovine cumulus cell molecular markers predictive of oocyte competence: functional and diagnostic implications. Biol Reprod 2008; 79(2): 301–9.CrossRefGoogle ScholarPubMed
Burns, KH, Owens, GE, Ogbonna, SC, Nilson, JH, Matzuk, MM.Expression profiling analyses of gonadotropin responses and tumor development in the absence of inhibins. Endocrinology 2003; 144(10): 4492–507.CrossRefGoogle ScholarPubMed
Fayad, T, Levesque, V, Sirois, J, Silversides, DW, Lussier, JG.Gene expression profiling of differentially expressed genes in granulosa cells of bovine dominant follicles using suppression subtractive hybridization. Biol Reprod 2004; 70(2): 523–33.CrossRefGoogle ScholarPubMed
Ferrari, S, Lattuada, D, Paffoni, A, et al. Procedure for rapid oocyte selection based on quantitative analysis of cumulus cell gene expression. J Assist Reprod Genet 2010; 27(7): 429–34.CrossRefGoogle ScholarPubMed
Feuerstein, P, Cadoret, V, Dalbies-Tran, R, et al. Gene expression in human cumulus cells: one approach to oocyte competence. Hum Reprod 2007; 22(12): 3069–77.CrossRefGoogle ScholarPubMed
Koks, S, Velthut, A, Sarapik, A, et al. The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles. Mol Hum Reprod 2010; 16(4): 229–40.CrossRefGoogle ScholarPubMed
Hamel, M, Dufort, I, Robert, C, et al. Identification of differentially expressed markers in human follicular cells associated with competent oocytes. Hum Reprod 2008; 23(5): 1118–27.CrossRefGoogle ScholarPubMed
Hamel, M, Dufort, I, Robert, C, et al. Genomic assessment of follicular marker genes as pregnancy predictors for human IVF. Mol Hum Reprod 2010; 16(2): 87–96.CrossRefGoogle ScholarPubMed
Hamel, M, Dufort, I, Robert, C, et al. Identification of follicular marker genes as pregnancy predictors for human IVF: new evidence for the involvement of luteinization process. Mol Hum Reprod 2010; 16(8): 548–56.CrossRefGoogle ScholarPubMed
Nivet AL, BunelA Labrecque, R, et al. FSH withdrawal improves developmental competence of oocytes in the bovine model. Reproduction 2012; 143(2): 165–71.CrossRefGoogle ScholarPubMed
Tatemoto, H, Terada, T.Time-dependent effects of cycloheximide and alpha-amanitin on meiotic resumption and progression in bovine follicular oocytes. Theriogenology 1995; 43(6): 1107–13.CrossRefGoogle ScholarPubMed
Tanghe, S, Van Soom, A, Nauwynck, H, Coryn, M, de Kruif, A.Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization. Mol Reprod Dev 2002; 61(3): 414–24.CrossRefGoogle ScholarPubMed
Tanghe, S, Van Soom, A, Mehrzad, J, et al. Cumulus contributions during bovine fertilization in vitro. Theriogenology 2003; 60(1): 135–49.CrossRefGoogle ScholarPubMed
Sutton, ML, Gilchrist, RB, Thompson, JG.Effects of in-vivo and in-vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum Reprod Update 2003; 9(1): 35–48.CrossRefGoogle Scholar
Matzuk, MM, Burns, KH, Viveiros, MM, Eppig, JJ.Intercellular communication in the mammalian ovary: oocytes carry the conversation. Science 2002; 296(5576): 2178–80.CrossRefGoogle ScholarPubMed
Atef, A, Francois, P, Christian, V, Marc-Andre, S.The potential role of gap junction communication between cumulus cells and bovine oocytes during in vitro maturation. Mol Reprod Dev 2005; 71(3): 358–67.CrossRefGoogle ScholarPubMed
Peddinti, D, Memili, E, Burgess, SC.Proteomics-based systems biology modeling of bovine germinal vesicle stage oocyte and cumulus cell interaction. PLoS One 2010; 5(6): e11240.CrossRefGoogle ScholarPubMed
Assidi, M, Dufort, I, Ali, A, et al. Identification of potential markers of oocyte competence expressed in bovine cumulus cells matured with follicle-stimulating hormone and/or phorbol myristate acetate in vitro. Biol Reprod 2008; 79(2): 209–22.CrossRefGoogle ScholarPubMed
Assou, S, Haouzi, D, Mahmoud, K, et al. A non-invasive test for assessing embryo potential by gene expression profiles of human cumulus cells: a proof of concept study. Mol Hum Reprod 2008; 14(12): 711–19.CrossRefGoogle ScholarPubMed
Assou, S, Haouzi, D, De Vos, J, Hamamah, S.Human cumulus cells as biomarkers for embryo and pregnancy outcomes. Mol Hum Reprod 2010; 16(8): 531–8.CrossRefGoogle ScholarPubMed
Assou, S, Anahory, T, Pantesco, V, et al. The human cumulus–oocyte complex gene-expression profile. Hum Reprod 2006; 21(7): 1705–19.CrossRefGoogle ScholarPubMed
Assidi, M, Montag, M, Van Der Ven, K, Sirard, MA.Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study. J Assist Reprod Genet 2011; 28(2): 173–81.CrossRefGoogle ScholarPubMed
Huang, Z, Wells, D.The human oocyte and cumulus cells relationship: new insights from the cumulus cell transcriptome. Mol Hum Reprod 2010; 16(10): 715–25.CrossRefGoogle ScholarPubMed
Reich, A, Klatsky, P, Carson, S, Wessel, G. The transcriptome of a human polar body accurately reflects its sibling oocyte. J Biol Chem 2011; 286(47):40743–9.CrossRefGoogle ScholarPubMed
Spitzer, D, Murach, KF, Lottspeich, F, Staudach, A, Illmensee, K.Different protein patterns derived from follicular fluid of mature and immature human follicles. Hum Reprod 1996;11(4): 798–807.CrossRefGoogle ScholarPubMed
Angelucci, S, Ciavardelli, D, Di Giuseppe, F, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta 2006; 1764(11): 1775–85.CrossRefGoogle ScholarPubMed
Jarkovska, K, Martinkova, J, Liskova, L, et al. Proteome mining of human follicular fluid reveals a crucial role of complement cascade and key biological pathways in women undergoing in vitro fertilization. J Proteome Res 2010; 9(3): 1289–301.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×