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Chapter 9 - In Vitro Fertilization (IVF)

from Section 2 - Assisted Reproductive Procedures

Published online by Cambridge University Press:  05 March 2021

Eliezer Girsh
Affiliation:
Barzilai Medical Center, Ashkelon
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Summary

In vitro fertilization (IVF) is a complex series of techniques used to help with fertility or prevent genetic problems and assist with the conception. During IVF, oocytes are collected from ovaries and fertilized by spermatozoa in a laboratory. The fertilization can be done using the patient’s oocytes and the partner’s sperm or donor oocytes and partner’s/donor sperm. All gametes (spermatozoa and oocytes) should be correctly prepared and selected before the initiation of the fertilization process.

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Publisher: Cambridge University Press
Print publication year: 2021

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References

Bjorndahl, L, Mortimer, D, Barratt, CLR, et al. Sperm preparation. In: A Practical Guide to Basic Laboratory Andrology, 1st ed. New York: Cambridge University Press. 2010; 167187.CrossRefGoogle Scholar
Levitas, E, Lunenfeld, E, Weiss, N, et al. Relationship between the duration of sexual abstinence and semen quality: analysis of 9,489 semen samples. Fertil. Steril. 2005; 83:16801683.CrossRefGoogle ScholarPubMed
Pons, I, Cercas, R, Villas, C, Braña, C, Fernández-Shaw, S. One abstinence day decreases sperm DNA fragmentation in 90 % of selected patients J. Assist. Reprod. Genet. 2013; 30:12111218.CrossRefGoogle ScholarPubMed
Bahadur, G, Almossawia, O, Zeirideen Zaid, R, et al. Semen characteristics in consecutive ejaculates with short abstinence in subfertile males. RBM Online 2016; 32:323328.Google ScholarPubMed
Henkel, RR, Schill, WB. Sperm preparation for ART. Reprod. Biol. Endocrinol. 2003; 108:122.Google Scholar
Makker, K, Agarwal, A, Sharma, R. Oxidative stress and male infertility. Indian J. Med. Res. 2009; 129:357367.Google ScholarPubMed
Oshio, S, Kaneko, S, Iizuka, R, Mohri, H. Effects of gradient centrifugation on human sperm. Arch. Androl. 1987; 19:8593.CrossRefGoogle ScholarPubMed
Bourne, H, Edgar, DH, Baker, HWG. Sperm preparation techniques. In: Gardner, DK, Weissman, A, Howles, CM, Shoham, Z, eds., Textbook of Assisted Reproductive Techniques: Laboratory and Clinical Perspectives, 2nd ed. USA: Informa Healthcare. 2004; 7991.Google Scholar
Sakkas, D, Manicardi, GC, Tomlinson, M, et al. The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum. Reprod. 2000; 15:11121116.CrossRefGoogle ScholarPubMed
Allamaneni, SS, Agarwal, A, Rama, S, Ranganathan, P, Sharma, RK. Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa. Asian J. Androl. 2005; 7:8692.CrossRefGoogle ScholarPubMed
May-Panloup, P, Chrétien, MF, Savagner, F, et al. Increased sperm mitochondrial DNA content in male infertility. Hum. Reprod. 2003; 18:550556.CrossRefGoogle ScholarPubMed
Jackson, RE, Bormann, CL, Hassun, PA, et al. Effects of semen storage and separation techniques on sperm DNA fragmentation. Fertil. Steril. 2010; 94:26262630.CrossRefGoogle ScholarPubMed
Menkveld, R, Stander, FS, Kotze, TJ, Kruger, TF, van Zyl, JA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum. Reprod. 1990; 5:586592.CrossRefGoogle ScholarPubMed
Bonde, JP, Ernst, E, Jensen, TK, et al. Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners. Lancet 1998; 352:11721177.CrossRefGoogle ScholarPubMed
Kruger, TF, Menkveld, R, Stander, FS, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil. Steril. 1986; 46:11181123.CrossRefGoogle ScholarPubMed
Jacobs, M, Stolwijk, AM, Wetzels, AM. The effect of insemination/injection time on the results of IVF and ICSI. Hum. Reprod. 2001; 16:17081713.CrossRefGoogle ScholarPubMed
Dozortsev, D, Nagy, P, Abdelmassih, S, et al. The optimal time for intracytoplasmic sperm injection in the human is from 37 to 41 hours after administration of human chorionic gonadotropin. Fertil. Steril. 2004; 82:14921496.CrossRefGoogle Scholar
Isiklar, A, Mercan, R, Balaban, B, et al. Impact of oocyte pre-incubation time on fertilization, embryo quality and pregnancy rate after intracytoplasmic sperm injection. RBM Online 2004; 8:682686.Google ScholarPubMed
Garor, R, Shufaro, Y, Kotler, N, et al. Prolonging oocyte in vitro culture and handling time does not compensate for a shorter interval from human chorionic gonadotropin administration to oocyte pickup. Fertil. Steril. 2015; 103:7275.CrossRefGoogle Scholar
Naji, O, Moska, N, Dajani, Y, et al. Early oocyte denudation does not compromise ICSI cycle outcome: a large retrospective cohort study. RBM Online 2018; 37:1824.Google Scholar
de Moura, BR, Gurgel, MC, Machado, SP, et al. Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality. JBRA Assist. Reprod. 2017; 21: 2730.CrossRefGoogle Scholar
Rienzi, L, Ubaldi, F. Oocyte retrieval and selection. In: Gardner, DK, Weissman, A, Howles, CM, Shoham, Z, eds., Textbook of Assisted Reproductive Technologies: Laboratory and Clinical Perspectives, 3rd ed. London: Informa Healthcare, 2009; 5101.Google Scholar
Rienzi, L, Vajta, G, Ubaldi, F. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum. Reprod. Update 2011; 17:3445.CrossRefGoogle ScholarPubMed
Lazzaroni-Tealdi, E, Barad, DH, Yu, Y, et al. Oocyte scoring system with better predictability of clinical IVF pregnancies than currently practiced embryo quality assessment. PLoS One 2015; 10: e0143632.CrossRefGoogle Scholar
Kilani, S, Cooke, S, Chapman, M. Time course of meiotic spindle development in MII oocytes. Zygote 2011; 19:5562.CrossRefGoogle ScholarPubMed
Alvarez, C, García-Garrido, C, Taronger, R, González de Merlo, G. In vitro maturation, fertilization, embryo development & clinical outcome of human metaphase-I oocytes retrieved from stimulated intracytoplasmic sperm injection cycles. Indian J. Med. Res. 2013; 137:331338.Google ScholarPubMed
Fauque, P, Guibert, J, Jouannet, P, Patrat, C. Successful delivery after the transfer of embryos obtained from a cohort of incompletely in vivo matured oocytes at retrieval time. Fertil. Steril. 2008; 89:991.e1–991.e4.CrossRefGoogle ScholarPubMed
Hyun, C-S, Cha, J-H, Son, W-Y, et al. Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum. Reprod. 2007; 22:19911995.CrossRefGoogle ScholarPubMed
Ebner, T, Moser, M, Sommergruber, M, Shebl, O, Tews, G. Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development. Hum. Reprod. 2006; 21:29722977.CrossRefGoogle ScholarPubMed
Parikh, FR, Nadkarni, SG, Naik, NJ, Naik, DJ, Uttamchandani, SA. Cumulus coculture and cumulus-aided embryo transfer increases pregnancy rates in patients undergoing in vitro fertilization. Fertil. Steril. 2006; 86:839847.CrossRefGoogle ScholarPubMed
Cohen, MR. Intrauterine insemination. Int. J. Fertil. 1962; 7:235240.Google ScholarPubMed
Sakhel, K, Schwarck, S, Ashraf, M, Abuzeid, M. Semen parameters as determinants of success in 1662 cycles of intrauterine insemination after controlled ovarian hyperstimulation. Fertil. Steril. 2005; 84:248249.CrossRefGoogle Scholar
Papillon-Smith, J, Baker, SE, Agbo, C, Dahan, MH. Pregnancy rates with intrauterine insemination: comparing 1999 and 2010 World Health Organization semen analysis norms. RBM Online 2015; 30:392400.Google ScholarPubMed
Marshburn, PB, Alanis, M, Matthews, ML, et al. A short period of ejaculatory abstinence before intrauterine insemination is associated with higher pregnancy rates. Fertil. Steril. 2010; 93:286288.CrossRefGoogle Scholar
Merviel, P, Heraud, MH, Grenier, N, et al. Predictive factors for pregnancy after intrauterine insemination (IUI): an analysis of 1038 cycles and a review of the literature. Fertil. Steril. 2010; 93:7988.CrossRefGoogle Scholar
Zhang, E, Tao, X, Xing, W, Cai, L, Zhang, B. Effect of sperm count on success of intrauterine insemination in couples diagnosed with male factor infertility. Mater Sociomed 2014; 26:321323.CrossRefGoogle ScholarPubMed
Jurema, MW, Vieira, AD, Bankowski, B, et al. Effect of ejaculatory abstinence period on the pregnancy rate after intrauterine insemination. Fertil. Steril. 2005; 84:678681.CrossRefGoogle ScholarPubMed
Speyer, BE, Abramov, B, Saab, W, et al. Factors influencing the outcome of intrauterine insemination (IUI): age, clinical variables and significant thresholds. J. Obstet. Gynaecol. 2013; 33:697700.CrossRefGoogle ScholarPubMed
van Noord-Zaadstra, BM, Looman, CW, Alsbach, H, et al. Delaying childbearing: effect of age on fecundity and outcome of pregnancy. BMJ 1991; 302:13611365.CrossRefGoogle ScholarPubMed
Goldman, MB, Thornton, KL, Ryley, D, et al. A randomized clinical trial to determine optimal infertility treatment in older couples: the Forty and Over Treatment Trial (FORT-T). Fertil. Steril. 2014; 101:15741581.CrossRefGoogle Scholar
Tomlinson, MJ, Amissah-Arthur, JB, Thompson, KA, Kasraie, JL, Bentick, B. Prognostic indicators for intrauterine insemination (IUI): statistical model for IUI success. Hum. Reprod. 1996; 11:18921896.CrossRefGoogle ScholarPubMed
Honda, T, Tsutsumi, M, Komoda, F, Tatsumi, K. Acceptable pregnancy rate of unstimulated intrauterine insemination: a retrospective analysis of 17,830 cycles. Reprod. Med. Biol. 2015; 14: 2732.CrossRefGoogle ScholarPubMed
Tonguc, E, Var, T, Onalan, G, et al. Comparison of the effectiveness of single versus double intrauterine insemination with three different timing regimens. Fertil. Steril. 2010; 94:12671270.CrossRefGoogle ScholarPubMed
Khalifa, Y, Redgment, CJ, Tsirigotis, M, Grudzinskas, JG, Craft, IL. The value of single versus repeated insemination in intra-uterine donor insemination cycles. Hum. Reprod. 1995; 10:153154.CrossRefGoogle ScholarPubMed
Trounson, AO. The choice of the most appropriate fertilization technique for human male factor infertility. Reprod. Fertil. Dev. 1994; 6:3743.CrossRefGoogle ScholarPubMed
Fiorentino, A, Magli, MC, Fortini, D, et al. Sperm:oocyte ratios in an in vitro fertilization (IVF) program. J. Assist. Reprod. Genet. 1994; 2:97103.CrossRefGoogle Scholar
Ron-el, R, Nachum, H, Herman, A, et al. Delayed fertilization and poor embryonic development associated with impaired semen quality. Fertil. Steril. 1991; 55:338344.CrossRefGoogle ScholarPubMed
Gianaroli, L, Tosti, E, Magli, MC, et al. Fertilization current in the human oocyte. Mol. Reprod. Dev. 1994; 3:209214.CrossRefGoogle Scholar
Bedford, JM, Kim, HH. Cumulus oophorus as a sperm sequestering device in vivo. J. Exp. Zool. 1993; 265:321328.CrossRefGoogle ScholarPubMed
Gianaroli, L, Fiorentino, A, Magli, MC, Ferraretti, AP, Montanaro, N. Prolonged sperm-oocyte exposure and high sperm concentration affect human embryo viability and pregnancy rate. Hum. Reprod. 1996; 11:25072511.CrossRefGoogle ScholarPubMed
Malter, HE, Cohen, J. Partial zona dissection of the human oocyte: a nontraumatic method using micromanipulation to assist zona pellucida penetration. Fertil. Steril. 1989; 51:139148.CrossRefGoogle ScholarPubMed
Laws-King, A, Trounson, A, Sathananthan, AH, Kola, I. Fertilisation of human oocytes by micro-injection of a single spermatozoon under the zona pellucida. Fertil. Steril. 1987; 48:637642.CrossRefGoogle Scholar
Ng, SC, Bongso, A, Ratnam, SS. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia. Fertil. Steril. 1991; 56:11171123.CrossRefGoogle ScholarPubMed
Sakkas, D, Gianaroli, L, Diotallevi, L, et al. IVF treatment of moderate male factor infertility: a comparison of mini-Percoll, partial zona dissection and sub-zonal sperm insertion techniques. Hum. Reprod. 1993; 8:587591.CrossRefGoogle ScholarPubMed
Levran, D, Bider, D, Yonesh, M, et al. A randomized study of intracytoplasmic sperm injection (ICSI) versus subzonal insemination (SUZI) for the management of severe male factor infertility. J. Assist. Reprod. Genet. 1995; 12:319321.CrossRefGoogle ScholarPubMed
Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Pregnancies after intra-cytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340:1718.CrossRefGoogle Scholar
Boulet, SL, Mehta, A, Kissin, DM, et al. Trends in use of and reproductive outcomes associated with intracytoplasmic sperm injection. JAMA 2015; 313:255263.CrossRefGoogle ScholarPubMed
Wang, J, Sauer, MV. In vitro fertilization (IVF): a review of 3 decades of clinical innovation and technological advancement. Ther. Clin. Risk Manag. 2006; 2:355364.CrossRefGoogle ScholarPubMed
Tsai, MY, Huang, FJ, Kung, FT, et al. Influence of polyvinylpyrrolidone on the outcome of intracytoplasmic sperm injection. J. Reprod. Med. 2000; 45:115120.Google ScholarPubMed
Dozortsev, D, Rybouchkin, A, De Sutter, P, Dhont, M. Sperm plasma membrane damage prior to intracytoplasmic sperm injection: a necessary condition for sperm nucleus decondensation. Hum. Reprod. 1995; 10:29602964.CrossRefGoogle ScholarPubMed
Rienzi, L, Ubaldi, F, Martinez, F, et al. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum. Reprod. 2003; 18:12891293.CrossRefGoogle ScholarPubMed
Palermo, GD. ICSI: technical aspects. In: Gardner, DK, Weissman, A, Howles, CM, Shoham, Z, eds., Textbook of Assisted Reproductive Techniques: Laboratory and Clinical Perspectives. Boca Raton: CRC Press. 2001; 147157.Google Scholar
Sousa, M, Tesarik, J. Ultrastructural analysis of fertilization failure after intracytoplasmic sperm injection. Hum. Reprod. 1994; 9:23742380.CrossRefGoogle ScholarPubMed
Flaherty, SP, Payne, D, Swann, NJ, Mattews, CD. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum. Reprod. 1995; 10:26232629.CrossRefGoogle ScholarPubMed
Ebner, T, Moser, M, Sommergruber, M. Jesacher, K, Tews, G. Complete oocyte activation failure after ICSI can be overcome by a modified injection technique. Hum. Reprod. 2004; 19:18371841.CrossRefGoogle ScholarPubMed
Bosco, L, Ruvolo, G, Morici, G, et al. Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection. Fertil. Steril. 2005; 84:14171423.CrossRefGoogle ScholarPubMed
Bárcena, P, Obradors, A, Vernaeve, V, Vassena, R. Should we worry about the clock? Relationship between time to ICSI and reproductive outcomes in cycles with fresh and vitrified oocytes. Hum. Reprod. 2016; 31:11821191.CrossRefGoogle ScholarPubMed
Van de Velde, H, De Vos, A, Joris, H, Nagy, ZP, Van Steirteghem, AC. Effect of timing of oocyte denudation and micro-injection on survival, fertilization and embryo quality after intracytoplasmic sperm injection. Hum. Reprod. 1998; 13:31603164.CrossRefGoogle ScholarPubMed
Reynier, P, May-Panloup, P, Chretien, MF, et al. Mitochondrial DNA content affects the fertilizability of human oocytes. Mol. Hum. Reprod. 2001; 7:425429.CrossRefGoogle ScholarPubMed
Celik-Ozenci, C, Jakab, A, Kovacs, T, et al. Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. Hum. Reprod. 2004; 19:20522059.CrossRefGoogle ScholarPubMed
Viville, S, Mollard, R, Bach, ML, et al. Do morphological anomalies reflect chromosomal aneuploidies?: case report. Hum. Reprod. 2000; 15:25632566.CrossRefGoogle ScholarPubMed
Zeyneloglu, HB, Baltaci, V, Duran, HE, Erdemli, E, Batioglu, S. Achievement of pregnancy in globozoospermia with Y chromosome microdeletion after ICSI. Hum. Reprod. 2002; 17:18331836.CrossRefGoogle ScholarPubMed
Stalf, T, Sánchez, R, Köhn, FM, et al. Pregnancy and birth after intracytoplasmic sperm injection with spermatozoa from a patient with tail stump syndrome. Hum. Reprod. 1995; 10:21122114.CrossRefGoogle ScholarPubMed
Okada, H, Fujioka, H, Tatsumi, N, et al. Assisted reproduction for infertile patients with 9 + 0 immotile spermatozoa associated with autosomal dominant polycystic kidney disease. Hum. Reprod. 1999; 14:110113.CrossRefGoogle ScholarPubMed
Tesarik, J, Rolet, F, Brami, C, et al. Spermatid injection into human oocytes. II. Clinical application in the treatment of infertility due to non-obstructive azoospermia. Hum. Reprod. 1996; 11:780783.CrossRefGoogle ScholarPubMed
Terriou, P, Hans, E, Giorgetti, C, et al. Pentoxifylline initiates motility in spontaneously immotile epididymal and testicular spermatozoa and allows normal fertilization, pregnancy, and birth after intracytoplasmic sperm injection. J. Assist. Reprod. Genet. 2000; 17:194199.CrossRefGoogle ScholarPubMed
Aktan, TM, Montag, M, Duman, S, et al. Use of a laser to detect viable but immotile spermatozoa. Andrologia 2004; 36:366369.CrossRefGoogle ScholarPubMed
Said, TM, Agarwal, A, Grunewald, S, et al. Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation. RBM Online 2006;13:336339.Google ScholarPubMed
Bartoov, B, Berkovitz, A, Eltes, F, et al. Real-time fine morphology of motile human sperm cells is associated with IVF–ICSI outcome. J. Androl. 2002; 23:18.CrossRefGoogle ScholarPubMed
Parmegiani, L, Cognigni, GE, Bernardi, S, et al. “Physiologic ICSI”: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil. Steril. 2010; 93:598604.CrossRefGoogle ScholarPubMed
Huszar, G, Ozkavukcu, S, Jakab, A, et al. Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr. Opin. Obstet. Gynecol. 2003; 18:260267.CrossRefGoogle Scholar
Yagci, A, Murk, W, Stronk, J, Huszar, G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. J. Androl. 2010; 31:566572.CrossRefGoogle ScholarPubMed
Chiou Fen, C, Ni Lee, S, Nee Lim, M, Ling Yu, S. Relationship between sperm hyaluronan-binding assay (HBA) scores on embryo development, fertilisation, and pregnancy rate in patients undergoing intra-cytoplasmic sperm injection (ICSI). Proc. Singapore Healthc. 2013; 22:120124.Google Scholar
Morozumi, K, Shikano, T, Miyazaki, S, Yanagimachi, R. Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development. Proc. Natl. Acad. Sci. U. S. A. 2006; 103:1766117666.CrossRefGoogle ScholarPubMed
Ramalho-Santos, J, Sutovsky, P, Simerly, C, et al. ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications. Hum. Reprod. 2000; 15:26102620.CrossRefGoogle ScholarPubMed
Rawe, VY, Olmedo, SB, Nodar, FN, et al. Cytoskeletal organization defects and abortive activation in human oocytes after IVF and ICSI failure. Mol. Hum. Reprod. 2000; 6:510516.CrossRefGoogle ScholarPubMed
Murase, Y, Araki, Y, Mizuno, S, et al. Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. Hum. Reprod. 2004; 19:16041607.CrossRefGoogle Scholar
Borges, E Jr, de Almeida Ferreira Braga, DP, de Sousa Bonetti, TC, Iaconelli, A Jr, Franco, JG Jr. Artificial oocyte activation using calcium ionophore in ICSI cycles with spermatozoa from different sources. RBM Online 2009; 18:4552.Google ScholarPubMed
Schmiady, H, Schulze, W, Scheiber, I, Pfüller, B. High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report. Hum. Reprod. 2005; 20:13191323.CrossRefGoogle ScholarPubMed
Wald, M, Ross, LS, Prins, GS, et al. Analysis of outcomes of cryopreserved surgically retrieved sperm for IVF/ICSI. J. Androl. 2006; 27:6065.CrossRefGoogle ScholarPubMed
Chapter 5: Sperm Preparation Techniques. In: Cooper, TG, Aitken, J, Auger, J, et al., eds., World Health Organization Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Switzerland: WHO Press. 2010; 161168.Google Scholar
Brackett, NL, Kafetsoulis, A, Ibrahim, E, Aballa, TC, Lynne, CM. Application of 2 vibrators salvages ejaculatory failures to 1 vibrator during penile vibratory stimulation in men with spinal cord injuries. J. Urol. 2007; 177:660663.CrossRefGoogle ScholarPubMed
Saito, K, Kinoshita, Y, Hosaka, M. Direct and indirect effects of electrical stimulation on the motility of human sperm. Int. J. Urol. 1999; 6:196199.CrossRefGoogle ScholarPubMed
Brackett, NL, Padron, OF, Lynne, CM. Semen quality of spinal cord injured men is better when obtained by vibratory stimulation versus electroejaculation. J. Urol. 1997; 157:151157.CrossRefGoogle ScholarPubMed
Hewitson, L, Simerly, C, Schatten, G. Cytoskeletal aspects of assisted fertilization. Semin. Reprod. Med. 2000;18:151159.CrossRefGoogle ScholarPubMed
Hewitson, L, Dominko, T, Takahashi, D, et al. Unique checkpoints during the first cell cycle of fertilization after intracytoplasmic sperm injection in rhesus monkeys. Nat. Med. 1999; 5:431433.CrossRefGoogle ScholarPubMed
Silber, SJ, Alagappan, R, Brown, LG, Page, DC. Y chromosome deletions in azoospermic and severely oligozoospermic men undergoing intracytoplasmic sperm injection after testicular sperm extraction. Hum. Reprod. 1998; 13:33323337.CrossRefGoogle ScholarPubMed
Page, DC, Silber, S, Brown, LG. Men with infertility caused by AZFc deletion can produce sons by intracytoplasmic sperm injection, but are likely to transmit the deletion and infertility. Hum. Reprod. 1999; 14:17221726.CrossRefGoogle ScholarPubMed
Tournaye, H, Devroey, P, Camus, M, et al. Comparision of in-vitro fertilization in male and tubal infertility: a 3 year survey. Hum. Reprod. 1992; 7:218222.CrossRefGoogle Scholar
Devroey, P. Clinical application of new micromanipulative technologies to treat the male. Hum. Reprod. 1998; 13:112122.CrossRefGoogle ScholarPubMed
Afzelius, BA, Eliasson, R. Male and female infertility problems in the immotile-cilia syndrome. Eur. J. Respir. Dis. Suppl. 1983; 127:144147.Google ScholarPubMed
Liu, J, Nagy, Z, Joris, H, et al. Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles. Hum. Reprod. 1995; 10:26302636.Google ScholarPubMed
Gul, U, Turunc, T, Haydardedeoglu, B, et al. Sperm retrieval and live birth rates in presumed Sertoli-cell-only syndrome in testis biopsy: a single centre experience. Andrology 2013; 1:4751.CrossRefGoogle ScholarPubMed
Oates, RD, Amos, JA. The genetic basis of congenital bilateral absence of the vas deferens and cystic fibrosis. J. Androl. 1994; 15:18.Google ScholarPubMed
Jarvi, K, Zielenski, J, Wilschanski, M, et al. Cystic fibrosis transmembrane conductance regulator and obstructive azoospermia. Lancet 1995; 345:1578.CrossRefGoogle ScholarPubMed
Antinori, S, Versaci, C, Dani, G, et al. Fertilization with human testicular spermatids: four successful pregnancies. Hum. Reprod. 1997; 12:286291.CrossRefGoogle ScholarPubMed
Araki, Y, Motoyama, M, Yoshida, A, et al. Intracytoplasmic injection with late spermatids: a successful procedure in achieving childbirth for couples in which the male partner suffers from azoospermia due to deficient spermatogenesis. Fertil. Steril. 1997; 67:559561.CrossRefGoogle ScholarPubMed
Hansen, M, Kurinczuk, JJ, Bower, C, Webb, S. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N. Engl. J. Med. 2002; 10:725730.CrossRefGoogle Scholar
Silber, S, Escudero, T, Lenahan, K, et al. Chromosomal abnormalities in embryos derived from testicular sperm extraction. Fertil. Steril. 2003; 79:3038.CrossRefGoogle ScholarPubMed
Doornbos, ME, Maas, SM, McDonnell, J, Vermeiden, JP, Hennekam, RC. Infertility, assisted reproduction technologies and imprinting disturbances. Hum. Reprod. 2007; 22:24762480.CrossRefGoogle ScholarPubMed
Van Steirteghem, A, Bonduelle, M, Devroey, P, Liebaers, I. Follow-up of children born after ICSI. Hum. Reprod. Update 2002; 8:111116.CrossRefGoogle ScholarPubMed
Palermo, GD, Colombero, LT, Schattman, GL, Davis, OK, Rosenwaks, Z. Evolution of pregnancies and initial follow-up of newborns delivered after intracytoplasmic sperm injection. JAMA 1996; 276:19831987.CrossRefGoogle ScholarPubMed
Bowen, JR, Gibson, FL, Leslie, GI, Saunders, DM. Medical and developmental outcome at 1 year for children conceived by intracytoplasmic sperm injection. Lancet 1998; 351:15291534.CrossRefGoogle ScholarPubMed
Bondulle, M, Joris, H, Hofmans, K, Liebaers, I, Van Steirteghem, A. Mental development of 201 ICSI children at 2 years of age. Lancet 1998; 351:1553.CrossRefGoogle Scholar
Sutcliffe, AG, Taylor, B, Li, J, et al. Children born after intracytoplasmic sperm injection: population control study. BMJ 1999; 318:704705.CrossRefGoogle ScholarPubMed
Winter, C, Van Acker, F, Bonduelle, M, Desmyttere, S, Nekkebroeck, J. Psychosocial development of full term singletons, born after preimplantation genetic diagnosis (PGD) at preschool age and family functioning: a prospective case-controlled study and multi-informant approach. Hum. Reprod. 2015; 3:11221136.CrossRefGoogle Scholar
DeBaun, MR, Niemitz, EL, Feinberg, AP. Association of in vitro fertilization with Beckwith–Wiedemann syndrome and epigenetic alterations of LIT1 and H19. Am. J. Hum. Genet. 2003; 72:156160.CrossRefGoogle ScholarPubMed

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