Published online by Cambridge University Press: 11 March 2010
These sperm processing procedures can generally be considered common to both intrauterine insemination (IUI) and assisted reproductive technology (ART) procedures.
Sperm washing is the primary method for seminal plasma removal from the sperm. Should difficulties arise, or sperm quality pose a further need for enhancement, then additional sperm treatment methods should be implemented.
In situations where sperm must be preserved for future use, semen cryopreservation is employed to freeze sperm for longterm storage:
Sperm washing procedure
Sperm washing, employing dilution and then centrifugation, is the primary procedure for separating sperm from seminal plasma.
The rationale for removing sperm from the seminal plasma is twofold:
Removal of decapacitation factor(s) and other detrimental elements; and
Concentration of all sperm within the ejaculate
Sperm washing for most semen samples begins with an initial four- to fivefold media dilution (see below) of the original seminal plasma, especially for those samples containing high sperm concentration or extremely high viscosity.
If a semen sample contains low sperm concentration in relatively high volume, then a relatively low dilution rate is necessary. Note that significantly high dilution procedures might require protein supplementation.
Media and protein supplementation
Recommendations for media and protein supplementation are somewhat arbitrary and subject to laboratory personnel opinion.
To save this book to your Kindle, first ensure email@example.com is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.
Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
Find out more about the Kindle Personal Document Service.
To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.
To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.