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Chapter 4 - Extended Semen Analysis

Published online by Cambridge University Press:  16 February 2022

David Mortimer
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
University of Dundee
José Antonio Castilla
HU Virgen de las Nieves, Granada
Roelof Menkveld
Stellenbosch University, South Africa
Ulrik Kvist
Karolinska Institutet, Stockholm
Juan G. Alvarez
Centro ANDROGEN, La Coruña
Trine B. Haugen
Oslo Metropolitan University
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Provides methods for a range of common additional assessments on semen that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes anti-sperm antobodies (direct and indirect MAR test), immunocytochemical staining of leukocytes, meaaurement of reactive oxygen species and seminal plasma redox state, and biochemical tests on the seminal plasma to evaluate acessory gland function (zinc for the prostate, frutose for the seminal vesicles and alpha-glusidase for the epidiymis) and investigate abnormalities of the sequence of ejaculation. There is also a discussion on the microbiological examination of semen,.

Publisher: Cambridge University Press
Print publication year: 2022

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