Published online by Cambridge University Press: 16 February 2022
Provides methods for a range of common additional assessments on semen that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes anti-sperm antobodies (direct and indirect MAR test), immunocytochemical staining of leukocytes, meaaurement of reactive oxygen species and seminal plasma redox state, and biochemical tests on the seminal plasma to evaluate acessory gland function (zinc for the prostate, frutose for the seminal vesicles and alpha-glusidase for the epidiymis) and investigate abnormalities of the sequence of ejaculation. There is also a discussion on the microbiological examination of semen,.