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Chapter 6 - Computer-Aided Sperm Analysis

Published online by Cambridge University Press:  16 February 2022

David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

Discusses the development, application and limitations of computer-aided sperm analysis (CASA) methods, including the deriation of kinematic measures of human sperm motility. Explains the technical and biological factors that limit CASA's functionality for human semen analysis and summarizes expert recommendations on the use of CASA for human semen analysis and sperm kinematics analysis (including sperm-mucus penetration and sperm hyperactivation). Issues related to the non-comparability of different CASA systems are considered, along with quality control for CASA. A strategy for validating a CASA system for human semen analysis, based on expectations of accuracy and precision, is also provided. Finally the use of CASA for analyzing sperm function tests, and new and future CASA technology (including the application of artificial intelligence technqiues) are surveyed.

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Publisher: Cambridge University Press
Print publication year: 2022

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