Published online by Cambridge University Press: 02 December 2021
Since its inception, ICSI has become the most widely used ART technique, and the ultimate treatment for severe male factor infertility. In this chapter, we provide fertilization and clinical pregnancy outcomes with ICSI utilizing oligo-, crypto- and astheno- zoospermic samples. We also describe the negative impact of ooplasmic dysmaturity on fertilization and propose methods to correct it. We also report the selection of spermatozoa with higher genomic integrity using a microfluidic chip in couples with high chromatin fragmentation and complete embryo aneuploidy. This will be followed by a discussion on the safety of ICSI in which we enlist various follow-up studies on the development and health of ICSI offspring through adulthood. To provide an overview on the widespread utilization of this procedure, we comment on the ICSI results reported worldwide.