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Cryobiology

Published online by Cambridge University Press:  06 July 2010

Ri-Cheng Chian
Affiliation:
McGill University, Montréal
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Summary

Cryobiology is the core of fertility cryopreservation. The principal application for human fertility cryopreservation began with sperm freezing, and then developed to include embryo and oocyte as well as gonadal cryopreservation. This chapter briefly discusses the scientific background and the current basic knowledge of cryobiology. Aqueous solutions are important for cryobiology since the freezing of biological systems always involves solutions containing substances such as electrolytes, non-electrolytes, polymers, and so on. Some of the classic papers in the field of cryobiology describe the theories and the mechanisms of cryoinjury during cell freezing and thawing. Some cryoprotectants reduce the injury of cells during freezing and thawing. Today, the most commonly used cryoprotectants in the field are glycerol, dimethyl sulfoxide, ethylene glycol, and propylene glycol. Cryoprotectants can interact with each other in a mixture, or with crucial cell molecules, thereby producing effects other than those that would occur with an individual cryoprotectant.
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Publisher: Cambridge University Press
Print publication year: 2010

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  • Cryobiology
  • Edited by Ri-Cheng Chian, McGill University, Montréal, Patrick Quinn
  • Book: Fertility Cryopreservation
  • Online publication: 06 July 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511730207.002
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  • Cryobiology
  • Edited by Ri-Cheng Chian, McGill University, Montréal, Patrick Quinn
  • Book: Fertility Cryopreservation
  • Online publication: 06 July 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511730207.002
Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

  • Cryobiology
  • Edited by Ri-Cheng Chian, McGill University, Montréal, Patrick Quinn
  • Book: Fertility Cryopreservation
  • Online publication: 06 July 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511730207.002
Available formats
×